Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   ACAM42_RS33140 Genome accession   NZ_AP028666
Coordinates   7157105..7157617 (-) Length   170 a.a.
NCBI ID   WP_369630677.1    Uniprot ID   -
Organism   Variovorax sp. V35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7152105..7162617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM42_RS33125 (GmRootV35_65540) - 7155475..7155837 (-) 363 WP_369630674.1 hypothetical protein -
  ACAM42_RS33130 (GmRootV35_65550) - 7155765..7156175 (-) 411 WP_369630675.1 hypothetical protein -
  ACAM42_RS33135 (GmRootV35_65560) - 7156193..7156453 (-) 261 WP_369630676.1 hypothetical protein -
  ACAM42_RS33140 (GmRootV35_65570) pilA2 7157105..7157617 (-) 513 WP_369630677.1 pilin Machinery gene
  ACAM42_RS33145 (GmRootV35_65580) - 7157820..7158623 (-) 804 WP_369632832.1 3',5'-cyclic-nucleotide phosphodiesterase -
  ACAM42_RS33150 (GmRootV35_65590) - 7158653..7160911 (-) 2259 WP_369630678.1 CHASE2 domain-containing protein -
  ACAM42_RS33155 (GmRootV35_65600) - 7160984..7161634 (-) 651 WP_095747310.1 FHA domain-containing protein -
  ACAM42_RS33160 (GmRootV35_65610) - 7161708..7162517 (-) 810 WP_070063289.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -

Sequence

Protein


Download         Length: 170 a.a.        Molecular weight: 16795.32 Da        Isoelectric Point: 9.6030

>NTDB_id=106797 ACAM42_RS33140 WP_369630677.1 7157105..7157617(-) (pilA2) [Variovorax sp. V35]
MNRRSIARNVQKGFTLIELMIVVAIIGILAAVALPAYQDYVVRSRVTEGLALASGAKTAISENAAQGAGFANGYVLPAAT
PNVSSIAIADLTGVITITYTAAAGNGTVTLTPTSGGTALAGTALTTAPTATVAGAGGSSTVPTGGSIEWLCRPGTTGGMA
VKFLPASCRP

Nucleotide


Download         Length: 513 bp        

>NTDB_id=106797 ACAM42_RS33140 WP_369630677.1 7157105..7157617(-) (pilA2) [Variovorax sp. V35]
ATGAACCGTCGTTCTATCGCACGCAATGTGCAAAAGGGTTTTACCCTGATCGAATTGATGATCGTTGTGGCGATCATTGG
TATCTTGGCCGCAGTTGCGCTGCCTGCGTACCAGGACTACGTTGTCCGCTCGCGCGTTACCGAAGGTCTGGCACTTGCCA
GCGGCGCCAAGACGGCAATCTCTGAAAACGCCGCCCAAGGCGCCGGCTTCGCGAATGGTTATGTGCTTCCGGCCGCAACG
CCAAATGTTTCGAGCATCGCCATCGCCGATCTGACTGGCGTGATCACGATTACCTACACGGCGGCAGCTGGCAACGGCAC
CGTCACTCTCACGCCGACGTCGGGCGGTACTGCTCTGGCTGGTACGGCGCTGACTACCGCGCCCACTGCCACGGTCGCCG
GCGCAGGCGGCAGCTCGACGGTTCCCACTGGTGGCTCCATTGAATGGCTGTGCCGTCCTGGCACGACCGGCGGCATGGCT
GTTAAGTTCCTGCCCGCTTCGTGCCGCCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-34)

Pilin (bacterial filament) (Pilin)

(42-169)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

53.333

97.059

0.518
  pilA2 Legionella pneumophila strain ERS1305867

53.333

97.059

0.518
  pilA Ralstonia pseudosolanacearum GMI1000

46.154

100

0.494
  comP Acinetobacter baylyi ADP1

44.172

95.882

0.424
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36

100

0.424
  pilA/pilA1 Eikenella corrodens VA1

40.828

99.412

0.406
  pilE Neisseria gonorrhoeae MS11

35.754

100

0.376

Multiple sequence alignment