Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACHFB9_RS03525 Genome accession   NZ_CP172404
Coordinates   746249..747964 (-) Length   571 a.a.
NCBI ID   WP_417069600.1    Uniprot ID   -
Organism   Niveibacterium terrae strain 24SJ04S-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 741249..752964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHFB9_RS03505 (ACHFB9_03505) - 743103..743714 (-) 612 WP_417069596.1 DUF502 domain-containing protein -
  ACHFB9_RS03510 (ACHFB9_03510) - 743729..743968 (-) 240 WP_417069597.1 FmdB family zinc ribbon protein -
  ACHFB9_RS03515 (ACHFB9_03515) pilD 744070..744921 (-) 852 WP_417069598.1 prepilin peptidase Machinery gene
  ACHFB9_RS03520 (ACHFB9_03520) pilC 744976..746208 (-) 1233 WP_417069599.1 type II secretion system F family protein Machinery gene
  ACHFB9_RS03525 (ACHFB9_03525) pilB 746249..747964 (-) 1716 WP_417069600.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACHFB9_RS03530 (ACHFB9_03530) - 748063..748869 (-) 807 WP_417069601.1 cytochrome C assembly family protein -
  ACHFB9_RS03535 (ACHFB9_03535) ffh 748917..750275 (+) 1359 WP_417069602.1 signal recognition particle protein -
  ACHFB9_RS03540 (ACHFB9_03540) - 750371..751627 (-) 1257 WP_417069603.1 D-amino acid dehydrogenase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62090.38 Da        Isoelectric Point: 5.1484

>NTDB_id=1067412 ACHFB9_RS03525 WP_417069600.1 746249..747964(-) (pilB) [Niveibacterium terrae strain 24SJ04S-2]
MVANPQQIPLSGLARALIQNQRISEQQALELTEKAKSADLPFAAALTQNGAMKARDLARFCAEMFGFPMLDLAAFDESQI
PRDAIDKKVVSSHLIVPLARKEKKITVGLADPTNLRALDEIRFQTGLAVEPVIVEIDKLLSLTKKLGESTSDALNNLTGN
IDEELGEVAGVETASEDENTSDVDDAPVVRFIQKMLVDAINEGASDIHFEPYEKYYRIRFRTDGILREISQPPLALREKI
AARIKVISRLDISEKRVPQDGRMKLKLSATKAIDFRVSTLPTLHGEKIVMRILDPSSATLGIEALGYEPEQQAALLEAVE
RPYGMVLVTGPTGSGKTVSLYTCLNILNKAGVNISTAEDPAEINLPGINQVNVNEKAGLTFSAALKSFLRQDPDVIMVGE
IRDLETAEIAIKAAQTGHMVLSTLHTNDAPTTLERLKNMGVAPFNIASSVIMITAQRLARRLCTCKKPVDIPIEALISAG
FTDEDLDGSWQPYGPVGCDRCKGSGYKGRVGIYQVMPIGEEIAHIIMTNGNSLDIAAEAQRQGIKDLRQSGLRKVKQGVT
SLAEVLACTND

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1067412 ACHFB9_RS03525 WP_417069600.1 746249..747964(-) (pilB) [Niveibacterium terrae strain 24SJ04S-2]
ATGGTCGCCAATCCTCAACAGATTCCGCTCAGTGGCCTCGCCCGCGCGCTGATCCAGAATCAACGCATCAGCGAACAGCA
AGCCCTCGAACTGACCGAAAAGGCCAAGTCCGCCGACCTGCCGTTCGCCGCCGCGCTGACTCAAAACGGCGCGATGAAGG
CACGGGATCTGGCGCGCTTCTGCGCCGAGATGTTCGGCTTTCCGATGCTGGACCTGGCCGCCTTCGACGAGAGCCAGATT
CCCCGCGATGCGATCGACAAGAAGGTGGTCAGCTCCCACCTGATCGTGCCGCTGGCCCGCAAAGAGAAAAAGATCACGGT
CGGACTGGCTGATCCGACCAATCTGCGTGCCCTCGACGAAATCCGCTTTCAAACCGGGCTGGCGGTCGAACCGGTAATCG
TCGAAATCGACAAGCTGCTGTCCCTGACCAAAAAGCTCGGCGAAAGCACCAGTGATGCGCTGAACAATCTGACCGGAAAC
ATCGACGAGGAGCTCGGCGAAGTCGCGGGCGTCGAAACAGCCAGCGAGGACGAGAACACCAGCGACGTCGATGACGCGCC
GGTCGTCCGCTTCATCCAGAAGATGCTCGTCGACGCGATCAACGAAGGCGCTTCCGACATCCACTTCGAGCCCTACGAGA
AGTACTACCGGATCCGCTTCCGCACCGACGGCATCCTGCGCGAGATTTCCCAGCCACCTCTGGCCCTGAGGGAGAAGATC
GCGGCGCGGATCAAGGTGATCTCCCGGCTGGACATCTCGGAGAAGCGGGTTCCTCAGGATGGCCGGATGAAGCTGAAGCT
GTCGGCGACCAAGGCGATCGATTTCCGGGTCAGCACGCTGCCAACCCTGCACGGCGAGAAGATCGTGATGCGTATCCTGG
ACCCGAGCTCGGCAACGCTGGGCATCGAGGCACTGGGCTATGAGCCGGAACAGCAGGCGGCACTGCTTGAAGCGGTCGAA
CGCCCTTACGGCATGGTTCTGGTCACCGGCCCGACCGGCTCGGGCAAGACCGTCTCGCTCTACACCTGCCTGAACATCCT
GAACAAGGCCGGGGTCAATATTTCGACCGCCGAGGATCCGGCCGAAATCAACCTGCCCGGCATCAACCAGGTCAACGTCA
ATGAAAAGGCGGGCCTGACCTTTTCGGCCGCACTCAAATCCTTCCTCCGCCAAGATCCGGATGTGATCATGGTCGGCGAA
ATCCGCGACCTGGAGACCGCCGAAATCGCGATCAAAGCGGCCCAGACCGGTCACATGGTGCTCTCGACGCTGCACACGAA
CGATGCACCGACGACGCTGGAGCGGCTCAAGAACATGGGGGTCGCGCCCTTCAACATCGCCTCCAGCGTCATCATGATCA
CCGCCCAGCGCCTCGCCCGACGCCTGTGCACCTGCAAAAAACCGGTCGATATCCCGATCGAGGCGCTGATCTCGGCGGGC
TTCACCGACGAGGATCTCGACGGCAGCTGGCAGCCTTACGGCCCGGTCGGCTGCGACCGCTGCAAGGGTTCAGGCTACAA
GGGGCGGGTCGGCATTTATCAAGTAATGCCTATCGGCGAGGAAATTGCTCATATAATAATGACAAACGGGAATTCCCTCG
ATATCGCGGCGGAAGCTCAGCGCCAGGGCATCAAGGATCTGCGGCAGTCCGGTCTGCGGAAAGTCAAACAGGGAGTCACG
TCGCTAGCCGAAGTGCTGGCATGCACCAACGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.36

99.124

0.559
  pilB Acinetobacter baylyi ADP1

56.106

98.949

0.555
  pilF Neisseria gonorrhoeae MS11

55.221

98.949

0.546
  pilB Legionella pneumophila strain ERS1305867

51.641

100

0.524
  pilB Vibrio cholerae strain A1552

48.577

98.424

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

48.479

97.898

0.475
  pilB Vibrio campbellii strain DS40M4

47.865

98.424

0.471
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.414

93.17

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

41.406

89.667

0.371

Multiple sequence alignment