Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACAM57_RS05875 Genome accession   NZ_AP028659
Coordinates   1204999..1206732 (-) Length   577 a.a.
NCBI ID   WP_126024679.1    Uniprot ID   -
Organism   Variovorax sp. V15     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1199999..1211732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM57_RS05855 (GmRootV15_11460) - 1200596..1202089 (-) 1494 WP_126024676.1 MBOAT family protein -
  ACAM57_RS05860 (GmRootV15_11470) coaE 1202159..1202770 (-) 612 WP_369660999.1 dephospho-CoA kinase -
  ACAM57_RS05865 (GmRootV15_11480) - 1202778..1203743 (-) 966 WP_184642162.1 A24 family peptidase -
  ACAM57_RS05870 (GmRootV15_11490) pilC 1203743..1204972 (-) 1230 WP_369661000.1 type II secretion system F family protein Machinery gene
  ACAM57_RS05875 (GmRootV15_11500) pilB 1204999..1206732 (-) 1734 WP_126024679.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAM57_RS05885 (GmRootV15_11510) - 1206977..1207969 (-) 993 WP_126024680.1 polyprenyl synthetase family protein -
  ACAM57_RS05890 (GmRootV15_11520) rplU 1208166..1208477 (+) 312 WP_019656543.1 50S ribosomal protein L21 -
  ACAM57_RS05895 (GmRootV15_11530) rpmA 1208499..1208759 (+) 261 WP_093057448.1 50S ribosomal protein L27 -
  ACAM57_RS05900 (GmRootV15_11540) cgtA 1208831..1209907 (+) 1077 WP_126024681.1 Obg family GTPase CgtA -
  ACAM57_RS05905 (GmRootV15_11550) proB 1209997..1211139 (+) 1143 WP_126024682.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62511.70 Da        Isoelectric Point: 5.4476

>NTDB_id=106722 ACAM57_RS05875 WP_126024679.1 1204999..1206732(-) (pilB) [Variovorax sp. V15]
MAAAELPVKEPSQIALPGLARALVSAGKLPARAAEDIYQKSISARTSFIAELTGSGAVSAADLAHTLSTAFGAPLLDLDA
IDPQRLPKDLLDQKLCHAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEAAAVSAAETI
NSIVGSEFEFDDVAAETANEGNDQAIAEVEDAPVVRFLHKMLLDAVSMRASDIHFEPYEHNYRVRFRVDGELREVASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARLGIDALGYDADEKAR
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCTVCKLPADIPR
ETLLEAGFKEEELDGSWKPYRPVGCSACSSGYKGRVGIYQVMPISEAIQEIILRDGSALDIAAQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVACTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=106722 ACAM57_RS05875 WP_126024679.1 1204999..1206732(-) (pilB) [Variovorax sp. V15]
ATGGCTGCAGCCGAACTTCCTGTTAAAGAACCTTCGCAAATTGCCCTACCCGGGCTTGCGCGCGCCCTGGTTTCGGCCGG
CAAGCTGCCTGCCAGGGCAGCCGAGGACATCTATCAGAAGTCCATCAGCGCTCGCACGAGCTTCATTGCTGAATTGACCG
GCTCCGGCGCCGTTTCCGCCGCAGACCTCGCCCACACCCTTTCCACCGCGTTCGGCGCACCGCTGCTCGATCTCGACGCC
ATCGATCCCCAGCGCCTGCCCAAGGACCTGCTCGACCAGAAGCTGTGCCATGCGTACCGCATCGTCGTGCTCAGCAAGCG
CAACAACCGTCTGATTGTTGCAACAGCCGATCCTTCCGATCAGCAGGCCGCGGAAAAGATCAAGTTCGCCTCCCAGATGG
GCGTCGACTGGGTCATCGCCGAATACGACAAGCTGTCGCGGATGATCGAAGCCGCCGCCGTCAGCGCGGCCGAGACCATC
AACAGCATCGTCGGCAGCGAGTTCGAGTTCGACGACGTCGCGGCCGAGACCGCGAACGAGGGCAATGACCAGGCCATCGC
CGAAGTCGAAGATGCGCCGGTCGTGCGCTTCCTGCACAAGATGCTGCTCGACGCCGTCAGCATGCGGGCATCCGACATCC
ACTTCGAGCCCTACGAGCACAACTACCGCGTCCGCTTCCGGGTCGACGGCGAGTTGCGCGAAGTCGCCAGCCCGCCCACG
GTCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTCATTTCCCGGCTCGACATCTCCGAGAAGCGCGTGCCGCAGGACGG
CCGCATGAAGCTCAAGATCGGTCCCGACCGCGTGATCGACTTCCGCGTGAGCACGCTGCCCACGCTCTTCGGCGAGAAGA
TCGTCGTGCGTATCCTCGACCCGAGCAGCGCGCGCCTGGGCATCGACGCCCTGGGTTACGACGCTGACGAGAAGGCGCGG
CTGCTGCATGCCATCGGCCGCCCGTACGGGATGGTGCTCGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTCTA
CACCTGCCTGAACCTCCTGAACCAGCCGGGCGTGAACATCGCGACGGCGGAAGACCCGTCGGAAATCAACCTGCCCGGCG
TCAACCAGGTCAACGTCAACGAACGCGCGGGCCTGACCTTCGCAGCCGCGCTGCGCGCCTTCCTGCGCCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCTCGATCAAGGCAGCCCAGACCGGCCACCTGGTGCT
CTCCACGCTGCACACCAACGATGCGCCGACCACGCTCACGCGGATGCGCAACATGGGCATCGCGCCGTTCAACATCGCGT
CGAGCGTGATCCTGATCACTGCCCAGCGCCTCGCGCGGCGCCTGTGCACCGTCTGCAAGCTGCCGGCCGACATCCCGCGC
GAAACGCTGCTCGAAGCAGGTTTCAAGGAAGAAGAACTGGACGGCTCCTGGAAGCCCTACCGCCCCGTCGGCTGCTCCGC
CTGCAGCAGCGGCTACAAGGGCCGCGTGGGCATCTACCAGGTGATGCCGATCTCGGAAGCCATCCAGGAAATCATCCTGC
GCGACGGCAGTGCGCTCGACATCGCCGCGCAGTCGGAAGCCGAGGGCGTGCGTTCGCTGCGCCAGTCGGGCCTGCGCAAG
GTCATGCAGGGCCTCACTTCGCTCGAAGAAGTGGTGGCCTGCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-153)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.516

97.4

0.541
  pilB Acinetobacter baylyi ADP1

54.545

97.227

0.53
  pilF Neisseria gonorrhoeae MS11

52.931

97.574

0.516
  pilB Legionella pneumophila strain ERS1305867

49.107

97.054

0.477
  pilB Vibrio campbellii strain DS40M4

45.633

97.227

0.444
  pilB Vibrio parahaemolyticus RIMD 2210633

45.422

96.534

0.438
  pilB Vibrio cholerae strain A1552

47.876

89.775

0.43
  pilF Thermus thermophilus HB27

38.529

98.96

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.037

93.068

0.373

Multiple sequence alignment