Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACH3PB_RS01720 Genome accession   NZ_CP172312
Coordinates   366942..368642 (-) Length   566 a.a.
NCBI ID   WP_416397751.1    Uniprot ID   -
Organism   Allohahella sp. A8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 361942..373642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH3PB_RS01690 (ACH3PB_01690) - 362290..363402 (-) 1113 WP_416397745.1 hypothetical protein -
  ACH3PB_RS01695 (ACH3PB_01695) - 363377..363838 (-) 462 WP_416397746.1 HIT family protein -
  ACH3PB_RS01700 (ACH3PB_01700) yacG 363835..364080 (-) 246 WP_416397747.1 DNA gyrase inhibitor YacG -
  ACH3PB_RS01705 (ACH3PB_01705) coaE 364077..364667 (-) 591 WP_416397748.1 dephospho-CoA kinase -
  ACH3PB_RS01710 (ACH3PB_01710) pilD 364698..365654 (-) 957 WP_416397749.1 prepilin peptidase Machinery gene
  ACH3PB_RS01715 (ACH3PB_01715) pilC 365658..366878 (-) 1221 WP_416397750.1 type II secretion system F family protein Machinery gene
  ACH3PB_RS01720 (ACH3PB_01720) pilB 366942..368642 (-) 1701 WP_416397751.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACH3PB_RS01725 (ACH3PB_01725) - 369063..369518 (+) 456 WP_416397752.1 pilin -
  ACH3PB_RS01730 (ACH3PB_01730) - 370057..371517 (+) 1461 WP_416397753.1 hypothetical protein -
  ACH3PB_RS01735 (ACH3PB_01735) - 371510..372367 (+) 858 WP_416397754.1 glycosyltransferase family 2 protein -
  ACH3PB_RS01740 (ACH3PB_01740) - 372381..373568 (-) 1188 WP_416397755.1 glycosyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62107.18 Da        Isoelectric Point: 5.6213

>NTDB_id=1066243 ACH3PB_RS01720 WP_416397751.1 366942..368642(-) (pilB) [Allohahella sp. A8]
MSAPLTGLARRFVFDGIIDEETARNASNEAKRANIPLVAYIVNNGLAKAPQIAQASSLEFGVPLFDLDALNLAVMPEKAI
GEKLVRKHQVLPLFKRGNRLFLAVADPTNIAAMDEIKFATGLNAEPVLVEQDKLTRAIDVYLDSQEQPLGGMDLDGDLED
IDIDTGDNKPEANQDSGEDDAPIVKYVNKMLLDAIKGGSSDVHFEPYEHAYRIRFRTDGILHEVSRPSVKLAAKFSARLK
VMAQLDISERRVPQDGRIKMKLSKTKSIDFRVNTLPTLWGEKIVLRILDASSAKMGIDALGYDPDQKQMYMDALAQPQGM
ILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGVNQVNVSTRVGLDFSAALKAFLRQDPDVVMVGEIRDLD
TASIAVKAAQTGHLVLSTLHTNSAPETLTRLRNMGVPSFNIATSVSLIIAQRLARRLCSECKEELKVPPEVLKEEGFTDE
QIATGFTLYHPKGCPKCKGGYRGRVGVYEVVRVTPELQELIMNDASSLEIARTAQKLGFRNLRQSALRKVIEGMTSLEDA
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1066243 ACH3PB_RS01720 WP_416397751.1 366942..368642(-) (pilB) [Allohahella sp. A8]
ATGAGCGCACCCCTTACAGGCCTTGCCCGTCGCTTTGTGTTCGACGGGATAATCGATGAAGAAACCGCTCGGAATGCCAG
CAATGAGGCCAAGCGCGCGAATATCCCGCTTGTTGCTTATATAGTAAACAATGGTCTCGCCAAGGCGCCCCAGATTGCCC
AAGCATCGTCACTCGAGTTTGGCGTACCACTTTTCGATCTTGACGCACTCAATCTTGCGGTCATGCCTGAGAAAGCAATC
GGCGAAAAGCTGGTCCGCAAGCACCAGGTTTTGCCTCTCTTTAAACGGGGAAACAGGCTATTTCTAGCAGTGGCGGATCC
CACCAATATTGCTGCGATGGACGAAATAAAGTTCGCGACAGGACTCAATGCAGAACCGGTGTTGGTCGAACAGGATAAGC
TCACTCGAGCAATCGATGTATATCTCGATAGCCAAGAACAACCTCTCGGCGGAATGGATCTCGACGGCGACCTTGAAGAT
ATCGACATCGATACTGGCGACAACAAGCCGGAGGCCAATCAGGATTCAGGTGAAGACGACGCGCCAATCGTCAAATATGT
GAACAAGATGCTATTGGACGCTATCAAGGGGGGCTCTTCCGACGTTCATTTCGAGCCCTACGAGCATGCCTATCGCATCC
GCTTCAGAACTGACGGTATTCTTCACGAGGTCTCTCGACCATCGGTCAAATTGGCTGCGAAATTCTCGGCCCGCTTGAAA
GTTATGGCCCAGCTCGACATCTCCGAACGTCGTGTCCCCCAAGACGGCCGGATCAAGATGAAACTGTCCAAGACCAAGTC
GATCGACTTCCGAGTCAACACGCTACCCACACTGTGGGGCGAAAAAATCGTTCTTCGAATTCTGGACGCCAGTAGCGCCA
AAATGGGCATCGATGCGCTGGGTTACGACCCGGACCAGAAGCAGATGTATATGGATGCGCTGGCGCAGCCACAAGGCATG
ATACTTGTCACAGGTCCTACTGGTAGTGGCAAAACGGTTTCGCTCTATACCGGCTTGAATATCCTTAACACTCCAGAGCG
GAACATTTCGACCGCCGAAGACCCTGTTGAGATTAACCTCGAAGGCGTCAACCAGGTGAATGTCAGTACTCGAGTCGGCC
TCGACTTCTCTGCTGCCCTCAAAGCCTTCCTTCGTCAGGATCCAGATGTGGTGATGGTGGGCGAGATTCGAGACCTTGAC
ACCGCCTCTATCGCAGTTAAGGCCGCTCAGACCGGACACCTCGTTTTATCTACTCTTCACACAAATAGCGCCCCAGAAAC
GCTCACCCGGCTGCGCAATATGGGTGTCCCTTCTTTCAATATCGCGACCTCGGTAAGCCTGATTATTGCGCAGCGTCTTG
CTCGGCGGCTCTGTAGCGAATGCAAGGAGGAATTGAAAGTCCCGCCAGAGGTATTAAAAGAGGAAGGCTTCACAGACGAA
CAAATTGCTACAGGGTTTACCTTGTATCATCCCAAGGGCTGTCCAAAATGTAAGGGCGGATATCGCGGTCGTGTGGGCGT
TTATGAAGTAGTTAGGGTCACGCCCGAGCTTCAGGAACTGATCATGAATGACGCCAGCTCGCTTGAGATTGCCAGGACGG
CGCAGAAACTGGGATTTCGTAACCTACGACAGTCCGCTCTGCGCAAGGTGATTGAAGGCATGACCAGCCTTGAAGATGCG
AACCGCGTGACGAAAGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.018

100

0.574
  pilB Acinetobacter baylyi ADP1

57.016

99.47

0.567
  pilB Legionella pneumophila strain ERS1305867

52.962

100

0.537
  pilB Vibrio cholerae strain A1552

52.987

91.696

0.486
  pilB Vibrio campbellii strain DS40M4

48.932

99.293

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

50

95.053

0.475
  pilF Neisseria gonorrhoeae MS11

47.359

100

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

40.927

91.519

0.375

Multiple sequence alignment