Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACF0H7_RS29390 Genome accession   NZ_CP172305
Coordinates   6314746..6316833 (-) Length   695 a.a.
NCBI ID   WP_397450300.1    Uniprot ID   -
Organism   Pseudomonas sp. NA-150     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6309746..6321833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF0H7_RS29375 (ACF0H7_29375) - 6311312..6312946 (-) 1635 WP_397450297.1 AAA family ATPase -
  ACF0H7_RS29380 (ACF0H7_29380) aroB 6312956..6314059 (-) 1104 WP_397450298.1 3-dehydroquinate synthase -
  ACF0H7_RS29385 (ACF0H7_29385) aroK 6314223..6314741 (-) 519 WP_397450299.1 shikimate kinase AroK -
  ACF0H7_RS29390 (ACF0H7_29390) pilQ 6314746..6316833 (-) 2088 WP_397450300.1 type IV pilus secretin PilQ Machinery gene
  ACF0H7_RS29395 (ACF0H7_29395) pilP 6316890..6317417 (-) 528 WP_397450301.1 type 4a pilus biogenesis lipoprotein PilP -
  ACF0H7_RS29400 (ACF0H7_29400) pilO 6317414..6318037 (-) 624 WP_397450302.1 type 4a pilus biogenesis protein PilO -
  ACF0H7_RS29405 (ACF0H7_29405) - 6318034..6318600 (-) 567 WP_397450303.1 PilN domain-containing protein -
  ACF0H7_RS29410 (ACF0H7_29410) comM 6318601..6319665 (-) 1065 WP_397450304.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74844.30 Da        Isoelectric Point: 5.2904

>NTDB_id=1066144 ACF0H7_RS29390 WP_397450300.1 6314746..6316833(-) (pilQ) [Pseudomonas sp. NA-150]
MTRIFSVIGISLWVAMVSPMLQAANLKTLDVAALPGDRVELKLTFDGPIPAPHGYTTEQPARIALDLPGVTSQLATKTRD
LSVGNARSLVVVEAKDRTRVIINMTALAPYSTRVEGNNLFVVVGQGASTAQRSQPTTAAGAARVSVPAVAPAKPRVAVGK
AIKNIDFQRGELGEGNVVIELSDSGIAPDIQEQGGKIRVDFAKTVLPDPLRVRLDVKDFATPVQFVTASAAGDKASINIE
PSGAFDYSAYQTDNKLTISVRPLTSEDVERRNADKLIYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLR
LQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQSVTS
AEAKTEERGSITVDERTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKALGVQWGGSTNRDGSGKWT
VAGNSQVTTPSGVTPNTNSPYVDLGAANATSGIGVSFLTDNILLDLQLSAMEKTGNGEVVSQPKVVTSDKETAKILKGTE
VPYQEASSSGATSVSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDYVNTVLGVPPIKKNEVNAKVLVSDGETIVIGGV
FSNTQSKTVEKVPFLGDVPYVGRLFRRDVVSEAKSELLVFLTPRIMNNQAVAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=1066144 ACF0H7_RS29390 WP_397450300.1 6314746..6316833(-) (pilQ) [Pseudomonas sp. NA-150]
ATGACTAGGATTTTCTCGGTTATCGGCATTTCGCTCTGGGTAGCGATGGTTTCGCCGATGCTCCAGGCGGCCAATCTTAA
GACGCTGGATGTCGCGGCGTTGCCTGGAGATCGCGTCGAGTTAAAGCTGACGTTTGACGGTCCGATTCCGGCACCGCATG
GCTATACCACCGAGCAGCCGGCGCGAATCGCCCTGGACCTGCCGGGTGTCACCAGCCAGTTGGCAACCAAGACCCGCGAC
CTGAGTGTGGGCAACGCCCGTAGCCTGGTGGTGGTCGAAGCCAAGGACCGCACGCGAGTCATCATCAACATGACAGCGCT
GGCCCCGTATAGCACGCGGGTCGAGGGTAATAACCTGTTTGTGGTGGTCGGGCAGGGCGCTTCAACCGCCCAGCGCTCGC
AACCAACCACCGCTGCCGGTGCCGCGCGCGTCTCGGTTCCGGCCGTGGCGCCAGCCAAGCCTCGCGTGGCGGTGGGCAAG
GCGATCAAGAATATCGACTTCCAGCGCGGTGAATTGGGCGAGGGTAACGTCGTCATCGAGCTGAGCGATTCGGGCATCGC
GCCCGACATCCAGGAGCAAGGCGGCAAGATTCGTGTCGACTTCGCCAAAACCGTGCTGCCAGACCCTCTGCGCGTGCGCC
TGGACGTCAAGGACTTCGCCACTCCGGTGCAGTTCGTCACCGCCAGCGCTGCGGGTGACAAGGCCAGCATCAACATTGAA
CCCAGCGGCGCCTTCGATTATTCGGCCTACCAGACCGACAACAAGCTGACCATCAGTGTGCGGCCGTTGACCTCGGAAGA
CGTCGAGCGGCGTAACGCCGACAAGCTGATCTACACCGGGGAAAAGCTGTCGCTGAACTTCCAGGACATCGACGTCCGCT
CGGTGCTGCAACTGATTGCGGACTTCACCAACCTCAACCTGGTCGCCAGTGACACCGTTCAAGGCGGGATCACCCTGCGC
CTGCAAAACGTGCCATGGGATCAAGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGACAAGCGCAAGATCGGCAACGT
GTTGCTGGTGGCGCCGGCTGACGAGATCGCTGCCCGTGAACGTCAGGAACTGGAGTCGTTGAAGCAGATTGCCGAATTGG
CGCCACTGCGTCGCGAGTTGTTGCAAGTGAACTACGCCAAGGCCGCCGACATCGCCAAACTGTTCCAGTCGGTGACCAGT
GCTGAAGCGAAAACCGAGGAACGTGGGTCGATCACCGTCGATGAGCGGACCAATAACATCATTGCCTACCAGACGCAGGA
TCGTCTCGACGAGTTGCGTCGTATCGTCTCGCAGCTGGACATCCCGGTACGTCAGGTGATGATCGAAGCCCGTATCGTCG
AAGCCAACGTCGGCTACGACAAGGCGCTCGGCGTGCAGTGGGGCGGTTCGACCAACCGTGACGGCAGCGGCAAGTGGACC
GTTGCCGGTAACAGCCAGGTGACTACGCCTAGCGGGGTCACGCCGAACACCAACTCTCCCTACGTCGACTTGGGCGCCGC
CAACGCTACCTCCGGGATCGGTGTCTCGTTCCTGACCGACAACATCCTGCTGGATCTGCAGCTCAGTGCGATGGAGAAGA
CCGGCAACGGTGAAGTCGTCTCGCAACCGAAAGTGGTCACCTCCGACAAGGAAACCGCGAAAATCCTCAAAGGCACCGAG
GTTCCGTATCAGGAAGCCAGCTCCAGCGGTGCGACGTCAGTGTCCTTCAAAGAGGCGGCGTTATCGCTGGAGGTGACGCC
GCAAATCACCCCGGACAACCGGATCATCATGGAAGTGAAAGTCACCAAGGATGAGCCGGATTATGTGAACACGGTGCTAG
GTGTGCCTCCGATCAAGAAAAACGAAGTGAACGCCAAGGTGCTGGTATCGGACGGCGAGACCATCGTAATTGGTGGTGTG
TTCTCCAATACGCAAAGTAAAACCGTTGAAAAGGTGCCATTCTTGGGCGATGTGCCGTATGTTGGCCGCCTTTTCCGGCG
CGACGTAGTTTCGGAAGCTAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGGTTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(380-445)

Secretin and TonB N terminus short domain (STN)

(306-352)

Bacterial type II and III secretion system protein (Secretin)

(530-686)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.713

100

0.763
  comQ Acinetobacter baylyi ADP1

42.672

100

0.427
  pilQ Acinetobacter baumannii strain A118

38.844

100

0.416
  pilQ Acinetobacter baumannii D1279779

38.844

100

0.416
  pilQ Legionella pneumophila strain Lp02

39.941

97.266

0.388
  pilQ Legionella pneumophila strain ERS1305867

39.941

97.266

0.388

Multiple sequence alignment