Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   ACF0H7_RS29410 Genome accession   NZ_CP172305
Coordinates   6318601..6319665 (-) Length   354 a.a.
NCBI ID   WP_397450304.1    Uniprot ID   -
Organism   Pseudomonas sp. NA-150     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6313601..6324665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF0H7_RS29385 (ACF0H7_29385) aroK 6314223..6314741 (-) 519 WP_397450299.1 shikimate kinase AroK -
  ACF0H7_RS29390 (ACF0H7_29390) pilQ 6314746..6316833 (-) 2088 WP_397450300.1 type IV pilus secretin PilQ Machinery gene
  ACF0H7_RS29395 (ACF0H7_29395) pilP 6316890..6317417 (-) 528 WP_397450301.1 type 4a pilus biogenesis lipoprotein PilP -
  ACF0H7_RS29400 (ACF0H7_29400) pilO 6317414..6318037 (-) 624 WP_397450302.1 type 4a pilus biogenesis protein PilO -
  ACF0H7_RS29405 (ACF0H7_29405) - 6318034..6318600 (-) 567 WP_397450303.1 PilN domain-containing protein -
  ACF0H7_RS29410 (ACF0H7_29410) comM 6318601..6319665 (-) 1065 WP_397450304.1 pilus assembly protein PilM Machinery gene
  ACF0H7_RS29415 (ACF0H7_29415) - 6319876..6322356 (+) 2481 WP_397454062.1 penicillin-binding protein 1A -
  ACF0H7_RS29420 (ACF0H7_29420) - 6322498..6323766 (-) 1269 WP_397450305.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38109.65 Da        Isoelectric Point: 4.7518

>NTDB_id=1066145 ACF0H7_RS29410 WP_397450304.1 6318601..6319665(-) (comM) [Pseudomonas sp. NA-150]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIADLDGVGHALSRLLIKAKTSAKSVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLEEVAIDFEVQGYSARNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSFGLLSAQLGHDHEQLTVAVVDIGATMTTLSVLHKGRIIYTREQLFGGRQLTEEIQRRYGLVM
EEAGLAKKQGGLPDDYVTEVLQPFKDAVVQQVSRSLQFFFAAGQYNTVDYIMLAGGTASIAGLDHLIQQRIGTPTLVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1066145 ACF0H7_RS29410 WP_397450304.1 6318601..6319665(-) (comM) [Pseudomonas sp. NA-150]
GTGTTCGAACTCTTCAGTAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTTGAATT
GAGTCGCTCCGGCACCCGCTACAAAGTTGAGTCCTATGCGGTCGAACCGCTGCCAGCCAACGCTGTGGTCGAAAAAAACA
TTGCTGACCTCGACGGGGTTGGCCATGCCTTGTCACGACTGCTGATAAAGGCCAAAACCAGCGCCAAAAGCGTTGCTGTC
GCCGTCGCTGGCTCAGCCGTGATCACCAAGACCATCGAGATGGACGCCGGCCTGTCGGACGACGACATGGAAAATCAGCT
GAAGATCGAAGCTGACCAGTACATCCCATACCCCCTCGAAGAAGTCGCCATCGATTTTGAAGTGCAAGGCTATTCGGCGC
GCAATCCCGAGCGCGTTGAAGTCTTGCTCGCTGCCTGCCGCAAGGAAAACGTCGAAGTGCGCGAGGCGGCCCTGGCGTTG
GCCGGCTTGACCGCCAAGGTCGTGGATGTCGAAGCGTATGCACTGGAACGCTCGTTCGGCCTGCTCTCGGCACAGTTGGG
GCACGATCACGAGCAACTGACGGTGGCGGTGGTCGACATCGGCGCGACCATGACCACCCTGAGCGTTCTGCACAAAGGCC
GGATCATCTACACCCGCGAACAATTGTTCGGCGGCCGTCAGCTGACCGAAGAAATTCAGCGTCGCTACGGTCTGGTGATG
GAAGAGGCCGGTCTGGCAAAAAAACAAGGCGGTCTGCCCGACGACTACGTCACTGAAGTGCTGCAACCGTTCAAGGACGC
GGTGGTACAGCAGGTATCGCGTTCACTGCAGTTCTTCTTCGCTGCCGGTCAGTACAACACCGTGGATTACATCATGCTGG
CGGGCGGCACCGCTTCGATCGCCGGTCTGGATCATTTGATTCAGCAGCGCATCGGCACGCCCACCCTGGTAGCGAACCCT
TTCGCCGATATGGCGCTAAGCAGCAAAGTCAACGCAGGGGCTCTGGCCAGCGATGCGCCGGCCTTGATGATTGCTTGTGG
TCTCGCGCTAAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.367

100

0.554
  pilM Acinetobacter baumannii D1279779

55.367

100

0.554
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475

Multiple sequence alignment