Detailed information NTG1066088

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ACF8YQ_RS12035
• Genome accession: NZ_CP172297
• Coordinates: 2600343..2602061 (+)
• Length: 572 a.a.
• NCBI ID: WP_396621537.1
• Uniprot ID: -
• Organism: Marinobacter sp. W-8
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2584717..2599435	2600343..2602061	flank	908

Gene organization within MGE regions

Location: 2584717..2602061

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF8YQ_RS11955	-	2584717..2585328 (+)	612	WP_396621523.1	c-type cytochrome	-
ACF8YQ_RS11960	-	2585415..2586116 (-)	702	WP_396621524.1	Fic family protein	-
ACF8YQ_RS11965	-	2586494..2586793 (-)	300	WP_023011844.1	CcdB family protein	-
ACF8YQ_RS11970	-	2586793..2587038 (-)	246	WP_228223898.1	type II toxin-antitoxin system CcdA family antitoxin	-
ACF8YQ_RS11975	-	2587204..2587752 (+)	549	WP_396621525.1	hypothetical protein	-
ACF8YQ_RS11980	-	2588056..2588361 (-)	306	WP_396621526.1	hypothetical protein	-
ACF8YQ_RS11985	-	2588573..2589694 (+)	1122	WP_396621527.1	UDP-N-acetylglucosamine 2-epimerase	-
ACF8YQ_RS11990	-	2589744..2590187 (+)	444	WP_396621528.1	glycosyltransferase	-
ACF8YQ_RS11995	-	2590180..2591205 (+)	1026	WP_396621529.1	glycosyltransferase family 4 protein	-
ACF8YQ_RS12000	-	2591558..2592223 (+)	666	WP_396621530.1	class I SAM-dependent methyltransferase	-
ACF8YQ_RS12005	-	2592220..2593122 (+)	903	WP_396621531.1	glycosyltransferase family 2 protein	-
ACF8YQ_RS12010	-	2593213..2594499 (-)	1287	WP_396621532.1	lipopolysaccharide biosynthesis protein	-
ACF8YQ_RS12015	-	2594560..2595423 (-)	864	WP_396621533.1	glycosyltransferase family 2 protein	-
ACF8YQ_RS12020	-	2595468..2597471 (-)	2004	WP_396621534.1	tetratricopeptide repeat protein	-
ACF8YQ_RS12025	-	2597468..2599435 (-)	1968	WP_396621535.1	tetratricopeptide repeat protein	-
ACF8YQ_RS12030	pilE	2599504..2599974 (-)	471	WP_396621536.1	pilin	Machinery gene
ACF8YQ_RS12035	pilB	2600343..2602061 (+)	1719	WP_396621537.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 572 a.a.
• Molecular weight: 62885.90 Da
• Isoelectric Point: 4.8638

>NTDB_id=1066088 ACF8YQ_RS12035 WP_396621537.1 2600343..2602061(+) (pilB) [Marinobacter sp. W-8]
MANSNRNVTLTGLARRFVDDGLLDEETAKDAFIQASQNRIPLITYLTQNQLADSSRLAFSAAMEFGVSVLDLDSFLPEMM
PEKVVDEKLVRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDSKLRAAIDKYLESQDTTMGDLDDA
DLEGVETEGGEGEDDDGAVSATDVDDAPIVKYVNKMLLDAIRGGASDIHFEPYEKIYRVRYRTDGILKEVSRPSIKLAPK
ISARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKKLYMDAL
AQPQGMILVTGPTGSGKTVSLYTGLNILNTPGINISTAEDPAEINLEGINQVNVNNRVGLGFAEALRAFLRQDPDVIMVG
EIRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCSSCKQPADVPKDVLLK
EGFTQEQIETGFTLYRPKGCDKCNGEYKGRVGIYEVVKITDELASMIMEEASSIKIAKQAQAEGFRNLRQSALLKVIEGV
TSLEEANRVTKD

Nucleotide

• Length: 1719 bp

>NTDB_id=1066088 ACF8YQ_RS12035 WP_396621537.1 2600343..2602061(+) (pilB) [Marinobacter sp. W-8]
ATGGCGAACAGCAACCGAAACGTGACTTTGACGGGCCTCGCCCGTCGTTTTGTGGACGACGGCCTTCTGGATGAAGAAAC
CGCAAAAGATGCATTTATCCAGGCATCTCAGAACCGAATTCCACTGATTACCTACCTGACCCAGAATCAACTTGCCGATA
GCTCCAGGCTCGCCTTCTCGGCAGCTATGGAATTCGGCGTTTCGGTATTGGACCTGGATTCCTTTCTTCCCGAAATGATG
CCGGAGAAGGTGGTCGACGAAAAACTGGTTCGCAAACATAACGCGCTCCCACTGTACAAAAGGGGTAACCGGCTATTTAT
CGCTGTTTCAGACCCCACCAATATTCAGGCTTTGGATGAAATCAAGTTCAATACCGGCCTGAGCACCGACGCGATTCTGG
TAGACGACTCAAAACTCCGCGCTGCTATCGACAAATATCTGGAGTCCCAGGACACCACAATGGGCGACTTGGACGATGCA
GATTTGGAAGGGGTCGAAACAGAAGGCGGTGAGGGTGAGGACGACGATGGCGCCGTATCCGCCACGGATGTTGATGATGC
GCCCATTGTTAAATATGTGAATAAGATGCTGCTGGATGCAATTCGGGGCGGGGCTTCGGATATTCACTTTGAGCCCTACG
AGAAGATCTACCGGGTTCGCTACCGGACCGATGGCATATTAAAAGAGGTATCTCGCCCCTCCATCAAGTTGGCGCCCAAG
ATCTCGGCCCGTGTAAAGATTATGGCGCAACTGGATATTTCTGAGCGTAGGGTGCCACAGGATGGCCGTATAAAAATGAA
GCTGTCCAAAACCAAGGCTATCGATTTCCGCGTAAATACGTTGCCAACGCTCTGGGGTGAAAAGATCGTTTTGCGGATTC
TGGATCCTAGTCAGGCAAAACTCGGCATTGACGTTCTGGGCTACGAGGAAGACCAGAAGAAGCTGTATATGGATGCTTTG
GCCCAGCCCCAGGGCATGATCCTAGTCACAGGGCCGACAGGTTCCGGTAAAACCGTATCGCTTTATACCGGCTTGAACAT
TTTGAACACCCCGGGCATCAACATTTCCACAGCGGAAGATCCGGCAGAGATCAACCTGGAAGGCATTAATCAGGTAAACG
TAAACAACCGAGTAGGGCTTGGCTTTGCCGAGGCACTGCGCGCCTTCCTGCGCCAAGACCCGGACGTCATCATGGTGGGT
GAGATCCGGGATCTGGAAACCGCCAACATTGCCATCAAAGCCGCACAGACCGGGCACCTGGTTCTATCTACCCTACACAC
TAACAGCGCCGCCGAGACTCTCACACGAATGATGAACATGGGGGTGCCCGCGTTTAACATAGCCACATCGGTAAGTTTGA
TTATTGCCCAACGACTGGGCCGACGACTCTGCAGCAGCTGCAAACAACCCGCCGATGTGCCTAAAGACGTGCTTTTGAAG
GAAGGGTTCACACAAGAACAAATTGAAACAGGCTTCACGTTGTACCGCCCCAAGGGCTGTGATAAATGCAATGGTGAATA
TAAAGGCCGCGTAGGTATTTACGAAGTGGTCAAGATCACCGACGAGTTGGCAAGCATGATTATGGAAGAGGCCAGCTCCA
TCAAAATTGCAAAGCAGGCTCAGGCGGAAGGCTTCCGTAATCTCAGGCAATCAGCGCTTCTCAAGGTTATTGAAGGAGTG
ACCAGCCTGGAAGAAGCCAACCGTGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (46-152)

• Type II/IV secretion system protein (T2SSE) (195-462)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.27	98.601	0.565
pilB	Acinetobacter baylyi ADP1	56.56	98.601	0.558
pilB	Legionella pneumophila strain ERS1305867	54.073	100	0.545
pilB	Vibrio parahaemolyticus RIMD 2210633	51.532	97.028	0.5
pilB	Vibrio cholerae strain A1552	54.528	90.734	0.495
pilB	Vibrio campbellii strain DS40M4	50.088	98.776	0.495
pilF	Neisseria gonorrhoeae MS11	48.057	98.951	0.476
pilB/pilB1	Synechocystis sp. PCC 6803	36.971	100	0.397
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.038	92.133	0.369
pilF	Thermus thermophilus HB27	37.184	96.853	0.36