Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACHWS3_RS09070 Genome accession   NZ_CP172104
Coordinates   1859118..1859552 (-) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain 681     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1854118..1864552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHWS3_RS09040 - 1854682..1854951 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  ACHWS3_RS09045 yacG 1854951..1855154 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  ACHWS3_RS09050 coaE 1855165..1855785 (-) 621 WP_014326298.1 dephospho-CoA kinase -
  ACHWS3_RS09055 - 1855800..1856522 (-) 723 WP_014326299.1 prepilin peptidase -
  ACHWS3_RS09060 - 1856522..1857733 (-) 1212 WP_014326300.1 type II secretion system F family protein -
  ACHWS3_RS09065 pilB 1857733..1859118 (-) 1386 WP_016504529.1 GspE/PulE family protein Machinery gene
  ACHWS3_RS09070 pilA 1859118..1859552 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  ACHWS3_RS09075 ampD 1859711..1860277 (+) 567 WP_014326302.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACHWS3_RS09080 rppH 1860863..1861459 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  ACHWS3_RS09085 - 1861462..1862256 (+) 795 WP_014326303.1 sulfite exporter TauE/SafE family protein -
  ACHWS3_RS09090 lgt 1862266..1863069 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  ACHWS3_RS09095 - 1863075..1863926 (+) 852 WP_005751072.1 thymidylate synthase -
  ACHWS3_RS09100 tadA 1863941..1864441 (+) 501 WP_016504277.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=1065176 ACHWS3_RS09070 WP_005741096.1 1859118..1859552(-) (pilA) [Pasteurella multocida strain 681]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1065176 ACHWS3_RS09070 WP_005741096.1 1859118..1859552(-) (pilA) [Pasteurella multocida strain 681]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCAGCTC
CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368

Multiple sequence alignment