Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACHWS3_RS09065 Genome accession   NZ_CP172104
Coordinates   1857733..1859118 (-) Length   461 a.a.
NCBI ID   WP_016504529.1    Uniprot ID   -
Organism   Pasteurella multocida strain 681     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1852733..1864118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHWS3_RS09035 - 1852860..1854233 (-) 1374 WP_014326297.1 alanine/glycine:cation symporter family protein -
  ACHWS3_RS09040 - 1854682..1854951 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  ACHWS3_RS09045 yacG 1854951..1855154 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  ACHWS3_RS09050 coaE 1855165..1855785 (-) 621 WP_014326298.1 dephospho-CoA kinase -
  ACHWS3_RS09055 - 1855800..1856522 (-) 723 WP_014326299.1 prepilin peptidase -
  ACHWS3_RS09060 - 1856522..1857733 (-) 1212 WP_014326300.1 type II secretion system F family protein -
  ACHWS3_RS09065 pilB 1857733..1859118 (-) 1386 WP_016504529.1 GspE/PulE family protein Machinery gene
  ACHWS3_RS09070 pilA 1859118..1859552 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  ACHWS3_RS09075 ampD 1859711..1860277 (+) 567 WP_014326302.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACHWS3_RS09080 rppH 1860863..1861459 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  ACHWS3_RS09085 - 1861462..1862256 (+) 795 WP_014326303.1 sulfite exporter TauE/SafE family protein -
  ACHWS3_RS09090 lgt 1862266..1863069 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  ACHWS3_RS09095 - 1863075..1863926 (+) 852 WP_005751072.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52173.85 Da        Isoelectric Point: 6.5002

>NTDB_id=1065175 ACHWS3_RS09065 WP_016504529.1 1857733..1859118(-) (pilB) [Pasteurella multocida strain 681]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIVLTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=1065175 ACHWS3_RS09065 WP_016504529.1 1857733..1859118(-) (pilB) [Pasteurella multocida strain 681]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGCGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGTTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTGAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAGGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCACAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGGATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTGCAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAACCTCAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGTGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.212

98.265

0.67
  pilB Haemophilus influenzae Rd KW20

67.55

98.265

0.664
  pilB Glaesserella parasuis strain SC1401

58.811

98.482

0.579
  pilB Acinetobacter baumannii D1279779

39.757

100

0.425
  pilB Vibrio campbellii strain DS40M4

39.419

100

0.412
  pilB Acinetobacter baylyi ADP1

37.876

100

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

39.293

100

0.41
  pilB Vibrio cholerae strain A1552

38.272

100

0.403
  pilB Legionella pneumophila strain ERS1305867

37.219

100

0.395

Multiple sequence alignment