Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACHMW6_RS04620 Genome accession   NZ_CP171977
Coordinates   850471..852165 (-) Length   564 a.a.
NCBI ID   WP_395405950.1    Uniprot ID   -
Organism   Pseudoduganella sp. UC29_106     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 845471..857165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMW6_RS04590 (ACHMW6_04580) - 846187..846583 (+) 397 Protein_813 NUDIX domain-containing protein -
  ACHMW6_RS04595 (ACHMW6_04585) yacG 846656..846838 (-) 183 WP_395405942.1 DNA gyrase inhibitor YacG -
  ACHMW6_RS04600 (ACHMW6_04590) zapD 846842..847597 (-) 756 WP_395405944.1 cell division protein ZapD -
  ACHMW6_RS04605 (ACHMW6_04595) coaE 847669..848292 (-) 624 WP_395405946.1 dephospho-CoA kinase -
  ACHMW6_RS04610 (ACHMW6_04600) pilD 848294..849166 (-) 873 WP_395408530.1 prepilin peptidase Machinery gene
  ACHMW6_RS04615 (ACHMW6_04605) pilC 849248..850459 (-) 1212 WP_395405948.1 type II secretion system F family protein Machinery gene
  ACHMW6_RS04620 (ACHMW6_04610) pilB 850471..852165 (-) 1695 WP_395405950.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACHMW6_RS04625 (ACHMW6_04615) - 852312..853589 (-) 1278 WP_395405952.1 HlyC/CorC family transporter -
  ACHMW6_RS04630 (ACHMW6_04620) - 853813..854559 (+) 747 WP_395405954.1 helix-turn-helix domain-containing protein -
  ACHMW6_RS04635 (ACHMW6_04625) - 854611..855102 (+) 492 WP_395405956.1 NuoB/complex I 20 kDa subunit family protein -
  ACHMW6_RS04640 (ACHMW6_04630) - 855146..856360 (+) 1215 WP_395405958.1 glutamate carboxypeptidase -
  ACHMW6_RS04645 (ACHMW6_04635) uraH 856463..856819 (-) 357 WP_395405960.1 hydroxyisourate hydrolase -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 61066.31 Da        Isoelectric Point: 4.9311

>NTDB_id=1064695 ACHMW6_RS04620 WP_395405950.1 850471..852165(-) (pilB) [Pseudoduganella sp. UC29_106]
MSGLARALLQAGRLSAPQADALQKKSQAEKQPFIDVLLASNAINARDLAMFCAETFAYPMLDLAAFNLDILPQKTIDPKL
MFGQRVIALSKRGNKMSVAISDPTNTQALDQIKFQTESTVEPVIVPHDTLVKLLADLSKSSEQALSDLSGDEGDIQFAED
DEAAPSQAQADAATEVEDAPIVRFLNKMLMDAVNMGASDLHFEPFEKFYRIRFRVDGVLVEHAQPPTGIKDKLISRIKVL
AKLDISEKRVPQDGRMRLIVSATKTIDLRISTLPTLFGEKCVMRILDATQAQMGIDSLGYDPDQKELLVDAIQRPYGMVL
VTGPTGSGKTVSLYSCLNILNKPGINISTAEDPAEINLPGVNQVNVNDKAGLTFPVALKAFLRQDPDIIMVGEIRDLETA
DIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLARRLCTCKQPVDISNDLLLDAGFKENDLD
GSWKPYGPVGCERCNGSGYKGRVGIYQIMPITPAIEALILANGNAMQIAAQSESEGVKSLRQSGLVKVKGGLTSLEEVLG
CTNE

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=1064695 ACHMW6_RS04620 WP_395405950.1 850471..852165(-) (pilB) [Pseudoduganella sp. UC29_106]
ATGTCGGGATTGGCTCGGGCATTGCTGCAGGCAGGGCGCCTCAGCGCCCCGCAGGCCGACGCGCTGCAGAAGAAATCCCA
GGCGGAAAAGCAGCCCTTCATTGACGTGCTCCTGGCAAGCAATGCCATCAATGCACGCGACCTGGCCATGTTCTGCGCCG
AGACTTTCGCCTATCCCATGCTGGATCTGGCCGCTTTCAATCTCGACATCCTGCCGCAGAAGACCATCGACCCCAAGCTG
ATGTTCGGCCAGCGCGTGATTGCACTGTCCAAACGCGGCAACAAGATGTCGGTGGCGATTTCCGATCCGACCAATACGCA
GGCGCTGGACCAGATCAAGTTCCAGACCGAGTCCACCGTGGAACCGGTCATCGTGCCGCACGATACCCTGGTCAAGCTGC
TGGCGGACTTGAGCAAGTCCTCGGAGCAGGCGCTGTCGGACCTGTCCGGCGATGAAGGCGATATCCAGTTCGCCGAGGAT
GACGAAGCCGCGCCAAGCCAGGCGCAGGCCGACGCCGCTACTGAAGTGGAAGACGCGCCGATCGTGCGCTTCCTGAACAA
GATGCTGATGGACGCCGTTAACATGGGTGCATCAGACTTGCATTTCGAGCCATTCGAAAAGTTCTACCGCATCCGCTTCC
GCGTGGACGGCGTGCTGGTCGAGCACGCGCAGCCGCCGACCGGCATCAAGGACAAACTGATCTCGCGCATCAAGGTGCTG
GCCAAGCTGGACATCTCCGAAAAGCGCGTGCCGCAGGACGGCCGCATGCGCCTGATCGTGTCGGCCACCAAGACCATCGA
TCTGCGTATCTCCACCCTGCCTACCCTGTTCGGCGAAAAGTGCGTGATGCGTATCCTGGACGCGACCCAGGCCCAGATGG
GCATCGATTCCTTGGGCTACGATCCGGACCAGAAGGAATTGCTGGTCGACGCCATCCAGCGCCCCTACGGCATGGTGCTG
GTGACCGGCCCCACCGGTTCGGGCAAGACGGTGTCCTTGTACTCCTGCCTGAACATCCTGAACAAACCCGGCATCAATAT
CTCCACCGCGGAAGACCCGGCCGAGATCAACCTGCCTGGCGTGAACCAGGTGAACGTGAACGACAAGGCGGGCCTGACCT
TTCCGGTCGCGCTGAAAGCCTTCCTGCGCCAGGACCCGGACATCATCATGGTCGGTGAGATTCGCGACCTGGAAACCGCC
GACATCGCGATCAAGGCCGCCCAGACCGGCCACATGGTGTTCTCCACCCTGCACACCAACGATGCGCCATCGACGCTGAC
GCGTCTGATGAATATGGGCGTGGCGCCCTTCAATATCGCTTCCTCCGTCATCCTGATCACGGCGCAGCGCCTGGCGCGCC
GCCTGTGCACCTGCAAGCAGCCGGTCGATATTTCAAACGACCTGCTGCTGGACGCCGGCTTCAAGGAGAACGACCTGGAT
GGCAGCTGGAAACCTTACGGCCCGGTGGGCTGTGAGCGCTGCAACGGATCCGGCTACAAGGGCCGGGTCGGTATCTACCA
GATCATGCCGATCACCCCCGCGATCGAGGCATTGATCCTGGCCAATGGCAATGCGATGCAAATCGCCGCGCAGTCCGAGT
CCGAAGGCGTTAAATCGCTGCGGCAATCGGGCCTGGTCAAGGTGAAGGGAGGCTTGACCAGCCTGGAGGAAGTGCTGGGC
TGCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(187-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.004

100

0.532
  pilF Neisseria gonorrhoeae MS11

52.641

100

0.53
  pilB Acinetobacter baylyi ADP1

52.12

100

0.523
  pilB Legionella pneumophila strain ERS1305867

49.027

100

0.491
  pilB Vibrio cholerae strain A1552

45.259

99.113

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

43.993

100

0.441
  pilB Vibrio campbellii strain DS40M4

43.186

100

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.123

100

0.395
  pilF Thermus thermophilus HB27

39.029

98.582

0.385

Multiple sequence alignment