Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACHFCC_RS02805 Genome accession   NZ_CP171806
Coordinates   587842..589560 (-) Length   572 a.a.
NCBI ID   WP_395140676.1    Uniprot ID   -
Organism   Schlegelella aquatica strain HS-12-14     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 582842..594560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHFCC_RS02775 (ACHFCC_02775) - 583349..583801 (+) 453 WP_395140664.1 NUDIX domain-containing protein -
  ACHFCC_RS02780 (ACHFCC_02780) - 583890..584126 (-) 237 WP_395140666.1 DNA gyrase inhibitor YacG -
  ACHFCC_RS02785 (ACHFCC_02785) zapD 584128..584880 (-) 753 WP_395140668.1 cell division protein ZapD -
  ACHFCC_RS02790 (ACHFCC_02790) coaE 585049..585651 (-) 603 WP_395140670.1 dephospho-CoA kinase -
  ACHFCC_RS02795 (ACHFCC_02795) pilD 585717..586574 (-) 858 WP_395140672.1 prepilin peptidase Machinery gene
  ACHFCC_RS02800 (ACHFCC_02800) pilC 586581..587801 (-) 1221 WP_395140674.1 type II secretion system F family protein Machinery gene
  ACHFCC_RS02805 (ACHFCC_02805) pilB 587842..589560 (-) 1719 WP_395140676.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACHFCC_RS02810 (ACHFCC_02810) recD 589758..591602 (-) 1845 WP_395140678.1 exodeoxyribonuclease V subunit alpha -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62471.75 Da        Isoelectric Point: 5.6481

>NTDB_id=1064286 ACHFCC_RS02805 WP_395140676.1 587842..589560(-) (pilB) [Schlegelella aquatica strain HS-12-14]
MDTLAEAPQSTLTGVARVLVNAGKLNARTAEELAKTAQDKRISFISAVIAAGAVSASDLAHALSQAFSLPLVDLNAIDLQ
RLPRGVVDNKLAAQYQVVVLGKRGSRLFVGGADPTNQEALERIKFATQLTPEWIIVEHDKLTKLLETMGTSAAEALESIA
GGDFEFDVSEEEDATQEAADVSTEVEDAPVVRFLQKMLIDAINMRASDLHFEPYEYNYRVRFRIDGELREITQPPIAIKD
KLASRIKVISKLDIAEKRVPQDGRMKLKFGNRAIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEKEEKERLLRAI
ERPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNISTVEDPAEINLPGINQVNVNDKAGLTFAAALKAFLRQDPDIIMVG
EIRDLETADIAIKAAQTGHLVMSTLHTNDAPTTLTRLRNMGVAPFNIASSVILITAQRLARRLCENCKTPAVYPREALLR
AGFKEEDLDGSWKPYRAVGCSSCNNGYKGRVGIYQVMPISEEIQRIILADGSAMDIAAQAQKEGVRDLRQAGLHKVKLGV
TSLEEVISVTNE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=1064286 ACHFCC_RS02805 WP_395140676.1 587842..589560(-) (pilB) [Schlegelella aquatica strain HS-12-14]
ATGGACACACTCGCTGAGGCTCCCCAAAGCACCCTGACCGGGGTGGCGCGGGTACTGGTCAACGCGGGCAAACTGAACGC
CCGCACCGCCGAAGAGCTCGCCAAGACCGCGCAGGACAAGCGCATCAGTTTCATCTCTGCCGTCATCGCGGCCGGCGCGG
TGTCGGCCAGCGATCTGGCCCATGCCCTTTCGCAGGCGTTCTCGCTGCCGCTGGTCGATCTCAATGCCATCGACCTGCAG
CGGCTGCCCCGAGGCGTCGTCGACAACAAGCTCGCCGCGCAGTACCAGGTGGTGGTGCTCGGCAAGCGCGGCAGCCGGCT
GTTCGTGGGCGGGGCGGACCCCACCAACCAGGAAGCGCTGGAGCGCATCAAGTTCGCCACCCAGCTCACGCCCGAATGGA
TCATCGTCGAGCACGACAAGCTCACCAAGCTGCTCGAGACGATGGGCACGAGTGCCGCGGAAGCACTCGAGAGCATCGCC
GGCGGGGACTTCGAGTTCGACGTCTCGGAGGAGGAGGACGCCACCCAGGAAGCGGCCGACGTCTCCACTGAGGTCGAGGA
CGCCCCCGTCGTGCGCTTCTTGCAGAAGATGCTGATCGACGCGATCAACATGCGCGCGTCCGACCTGCACTTCGAGCCCT
ACGAATACAACTACCGGGTGCGCTTCCGCATCGACGGCGAACTGCGCGAGATCACCCAGCCGCCGATCGCCATCAAGGAC
AAGCTCGCCTCGCGCATCAAGGTCATCTCCAAGCTCGACATCGCCGAAAAGCGCGTTCCGCAAGACGGGCGCATGAAGCT
CAAGTTCGGCAACCGCGCGATCGACTTCCGGGTCAGCACCCTGCCCACGCTGTTCGGCGAGAAGATCGTGATCCGTATCC
TGGATCCGTCGTCCGCCAAGCTCGGCATCGACGCGCTGGGCTACGAGAAGGAGGAAAAGGAGCGCCTGCTGCGCGCCATC
GAGCGGCCGTACGGGATGATCCTGGTCACCGGCCCCACCGGCAGCGGCAAGACGGTCTCGCTCTACACCTGCCTGAACCT
GCTCAACAAGCCGGGCGTCAACATCTCCACCGTCGAAGATCCGGCGGAAATCAACCTGCCCGGCATCAACCAGGTCAACG
TCAACGACAAGGCCGGCCTGACGTTCGCTGCCGCGCTCAAGGCCTTTCTGCGGCAGGACCCGGACATCATCATGGTGGGT
GAGATCCGCGACCTGGAGACGGCCGACATCGCGATCAAGGCGGCGCAAACCGGTCACCTGGTGATGTCCACGCTGCACAC
CAACGACGCGCCGACGACGCTGACGCGTCTGCGCAACATGGGCGTCGCACCGTTCAACATCGCGTCCAGCGTGATCCTCA
TCACCGCCCAGCGCCTGGCGCGCCGCCTGTGCGAGAACTGCAAGACGCCCGCGGTGTACCCGCGCGAGGCCCTGCTGCGC
GCGGGCTTCAAGGAAGAAGATCTGGACGGCAGCTGGAAGCCGTACCGCGCCGTGGGCTGCTCCAGCTGCAACAATGGCTA
CAAGGGCCGCGTCGGCATCTACCAGGTCATGCCGATCTCGGAGGAGATCCAGCGCATCATCCTGGCCGACGGCAGCGCGA
TGGACATCGCGGCACAGGCCCAGAAGGAGGGCGTGCGGGACTTGCGACAGGCGGGCCTGCACAAGGTGAAGCTCGGCGTC
ACCTCGCTCGAGGAAGTCATCTCGGTCACGAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.704

100

0.549
  pilB Acinetobacter baylyi ADP1

54.529

98.427

0.537
  pilF Neisseria gonorrhoeae MS11

54.352

98.427

0.535
  pilB Legionella pneumophila strain ERS1305867

50.704

99.301

0.503
  pilB Vibrio cholerae strain A1552

47.321

97.902

0.463
  pilB Vibrio campbellii strain DS40M4

46.607

97.902

0.456
  pilB Vibrio parahaemolyticus RIMD 2210633

46.263

98.252

0.455
  pilF Thermus thermophilus HB27

38.516

98.951

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.789

93.007

0.379

Multiple sequence alignment