Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACG4IV_RS08400 Genome accession   NZ_CP171688
Coordinates   1885868..1887094 (+) Length   408 a.a.
NCBI ID   WP_000648511.1    Uniprot ID   Q9X4G9
Organism   Vibrio cholerae strain M647     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1880868..1892094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG4IV_RS08375 fldB 1880904..1881422 (+) 519 WP_000690112.1 flavodoxin FldB -
  ACG4IV_RS08380 ampD 1881700..1882245 (-) 546 WP_000567318.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACG4IV_RS08385 nadC 1882541..1883431 (+) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACG4IV_RS08390 pilA 1883676..1884137 (+) 462 WP_000649326.1 pilin Machinery gene
  ACG4IV_RS08395 pilB 1884137..1885825 (+) 1689 WP_000957200.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACG4IV_RS08400 pilC 1885868..1887094 (+) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  ACG4IV_RS08405 pilD 1887152..1888027 (+) 876 WP_000418747.1 A24 family peptidase Machinery gene
  ACG4IV_RS08410 coaE 1888024..1888632 (+) 609 WP_000011557.1 dephospho-CoA kinase -
  ACG4IV_RS08415 zapD 1888664..1889404 (+) 741 WP_000207198.1 cell division protein ZapD -
  ACG4IV_RS08420 yacG 1889546..1889743 (+) 198 WP_000162868.1 DNA gyrase inhibitor YacG -
  ACG4IV_RS08425 parC 1889792..1892077 (-) 2286 WP_000102510.1 DNA topoisomerase IV subunit A -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45134.43 Da        Isoelectric Point: 10.3424

>NTDB_id=1063298 ACG4IV_RS08400 WP_000648511.1 1885868..1887094(+) (pilC) [Vibrio cholerae strain M647]
MKATQTLPLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKSKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAAAIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPILASLKTTAKTSGNVHFETAINEVYRDTAA
GMPMYIAMRNTEAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=1063298 ACG4IV_RS08400 WP_000648511.1 1885868..1887094(+) (pilC) [Vibrio cholerae strain M647]
ATGAAAGCGACCCAAACCTTACCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGCCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAGCTCAAAGATCAGCATATTCAGATCAAAAAACTCAAAAAAGGCA
GTGTATCTCTTTTGGCACGCCTAACCCATCGCGTGAAAAGTAAAGATATTACGATTTTGACTCGGCAGTTGGCGACCATG
CTCACCACGGGCGTACCCATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GGCGCAAATCACCAAAAGCGTGGAAGCGGGCACGCCACTTTCCAAGGCGATGCGCACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGATTTAGTGGAAACCGGAGAGATGTCCGGTAACTTACCTGAGGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAGCAACTACGCGCCAAGGTGATTAAAGCGCTCATCTACCCCAGCATGGTTGTGTTGGTCGCGCTCGGGGTATC
TTACTTAATGCTCACCATGGTCATCCCAGAGTTTGAAAGCATGTTTAAAGGCTTTGGTGCTGAACTGCCTTGGTTTACGC
AGCAAGTGCTGAAACTCTCACACTGGGTGCAGGCTTACAGTTTATGGGCATTTATCGCCATCGCAGCAGCCATTTTTGGC
TTGAAAGCGCTGCGTAAAAACTCTTTCCAGATCCGTTTAAAAACCAGCCGCTTAGGGCTGAAATTTCCGATTATTGGTAA
TGTGCTCGCTAAGGCTTCCATCGCCAAATTCAGCCGTACCCTCGCCACCAGCTTTGCCGCGGGGATCCCAATTCTCGCCA
GTTTAAAAACCACGGCCAAAACCTCCGGCAATGTGCACTTTGAAACCGCGATTAATGAGGTGTACCGCGATACCGCTGCG
GGTATGCCGATGTACATTGCTATGCGCAATACCGAAGCTTTTCCCGAAATGGTGCTGCAAATGGTGATGATCGGTGAAGA
GTCTGGGCAATTAGACGACATGCTCAACAAGGTCGCGACCATCTATGAATTTGAAGTCGATAACACGGTCGATAACTTGG
GCAAGATTCTTGAACCACTGATCATCGTCTTTCTTGGGACGGTTGTGGGCGGCTTAGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

100

100

1
  pilC Vibrio campbellii strain DS40M4

73.75

98.039

0.723
  pilC Acinetobacter baumannii D1279779

42.647

100

0.426
  pilC Acinetobacter baylyi ADP1

42.015

99.755

0.419
  pilC Legionella pneumophila strain ERS1305867

42.211

97.549

0.412
  pilC Pseudomonas stutzeri DSM 10701

42.172

97.059

0.409
  pilG Neisseria gonorrhoeae MS11

41.294

98.529

0.407
  pilG Neisseria meningitidis 44/76-A

40.796

98.529

0.402

Multiple sequence alignment