Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACGHX3_RS15410 Genome accession   NZ_CP171632
Coordinates   3290306..3292039 (+) Length   577 a.a.
NCBI ID   WP_394695526.1    Uniprot ID   -
Organism   Pseudoxanthomonas japonensis strain LY0623     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3285306..3297039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHX3_RS15390 (ACGHX3_15390) - 3286221..3287468 (-) 1248 WP_394695522.1 lipopolysaccharide biosynthesis protein -
  ACGHX3_RS15395 (ACGHX3_15395) - 3287465..3288739 (-) 1275 WP_394695523.1 glycosyltransferase family 4 protein -
  ACGHX3_RS15400 (ACGHX3_15400) - 3288822..3289673 (-) 852 WP_394695524.1 glycosyltransferase family 2 protein -
  ACGHX3_RS15405 (ACGHX3_15405) - 3289886..3290215 (+) 330 WP_394695525.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACGHX3_RS15410 (ACGHX3_15410) pilB 3290306..3292039 (+) 1734 WP_394695526.1 type IV pilus assembly protein Machinery gene
  ACGHX3_RS15415 (ACGHX3_15415) pilC 3292307..3293569 (+) 1263 WP_394695527.1 type II secretion system F family protein Machinery gene
  ACGHX3_RS15420 (ACGHX3_15420) pilD 3293573..3294436 (+) 864 WP_394695528.1 A24 family peptidase Machinery gene
  ACGHX3_RS15425 (ACGHX3_15425) coaE 3294569..3295186 (+) 618 WP_394695529.1 dephospho-CoA kinase -
  ACGHX3_RS15430 (ACGHX3_15430) - 3295508..3296482 (-) 975 WP_394695530.1 bifunctional MutT family pyrophosphohydrolase/thiamine phosphate synthase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62600.72 Da        Isoelectric Point: 5.9570

>NTDB_id=1062419 ACGHX3_RS15410 WP_394695526.1 3290306..3292039(+) (pilB) [Pseudoxanthomonas japonensis strain LY0623]
MNAVASANLVGITGIARRLVQDGALDEAVARTAMASATEAKVPLPQWIAEKRLVTAPQLAAANAMEFGMSLLDASAFDPT
HNALKLVSEELVQKHQVLPLYKRGGKLFVGVSDPTRSHSALDEIKFHTNLIVEPILVDEDQLRRTLDQWQNAAGALASNF
GEDDDGLDSLDVDAGDEDGGGDSAVDAKGDDTPVVKFVNKVLVDAIKRGASDIHFEPYETDYRVRLRIDGILKQVAKAPT
KLKDRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPDQQKL
FEDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNRRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCPKCKRPAQLPP
QALLAEGFTEAEIASGFAVYEAVGCDECTSGYKGRTGIYQVMPMSEAIQEIVLEGGNALQIAEVAQKAGIRDLRQSALLK
VRNGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1062419 ACGHX3_RS15410 WP_394695526.1 3290306..3292039(+) (pilB) [Pseudoxanthomonas japonensis strain LY0623]
ATGAATGCAGTCGCCTCCGCCAATCTCGTCGGCATCACCGGCATTGCCCGCCGACTGGTCCAGGACGGCGCGCTGGATGA
GGCTGTCGCCCGCACGGCGATGGCCAGCGCCACCGAAGCCAAGGTGCCGCTGCCGCAGTGGATCGCGGAAAAGCGGCTGG
TCACCGCCCCGCAGCTGGCGGCCGCGAATGCCATGGAATTCGGCATGTCGCTGCTGGATGCCTCTGCCTTCGACCCTACG
CACAACGCACTCAAGCTGGTCAGCGAGGAACTGGTGCAGAAGCACCAGGTGCTGCCGCTGTACAAGCGCGGCGGCAAGCT
GTTCGTCGGCGTGTCGGATCCCACCCGCAGCCATTCGGCGCTGGACGAGATCAAGTTCCACACCAACCTGATCGTCGAGC
CGATCCTGGTCGATGAGGACCAGTTGCGACGGACGCTGGACCAGTGGCAGAACGCGGCCGGTGCATTGGCCAGCAACTTC
GGCGAAGATGACGACGGCCTGGACAGCCTGGACGTGGACGCGGGGGACGAGGACGGCGGTGGCGACAGCGCCGTGGATGC
GAAAGGCGACGACACGCCCGTGGTCAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCAAGCGCGGCGCCTCGGACATCC
ACTTCGAGCCCTACGAGACCGACTACCGCGTGCGCCTGCGCATCGACGGCATCCTCAAGCAGGTGGCCAAGGCGCCGACC
AAGCTGAAGGACCGCATCGCGGCCCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGCGTGCCGCAGGACGG
ACGCATCAAGCTGAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCCACGCTGTTCGGCGAAAAGA
TCGTGCTGCGTATCCTGGACGGCAGCGCCGCGAAACTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAGCAGAAGCTG
TTCGAGGACGCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTCAGCCTGTA
CACCGCGCTGGGCATCCTGAACGACGAGACCCGCAACATCTCCACCGCGGAAGATCCGGTGGAAATCCGCCTGCCGGGCG
TCAACCAGGTGCAGCAGAACAACCGCCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGCCAGGATCCGGAC
ATCATCATGGTGGGCGAAATCCGCGACCTGGAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGCCACATGGTGCT
GTCCACCCTGCATACGAACGATGCCCCGCAGACTATCGCGCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACAA
GCTCGGTGACGCTGGTCATCGCCCAGCGCCTGGCGCGCCGGCTGTGCCCCAAGTGCAAGCGTCCGGCGCAGTTGCCGCCG
CAGGCCCTGCTCGCCGAGGGATTCACCGAGGCGGAGATCGCGAGCGGCTTCGCCGTCTATGAGGCCGTCGGCTGCGATGA
GTGCACCAGTGGCTACAAGGGCCGGACGGGCATCTACCAGGTGATGCCGATGAGCGAGGCGATCCAAGAGATCGTGCTGG
AAGGCGGCAACGCCCTGCAGATCGCCGAGGTGGCCCAGAAGGCCGGTATCCGCGACCTGCGCCAGTCGGCGCTGCTGAAG
GTGCGCAACGGCATCACCAGCCTGGCCGAGATCAACCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.732

98.267

0.548
  pilB Acinetobacter baylyi ADP1

55.203

98.267

0.542
  pilB Legionella pneumophila strain ERS1305867

52.289

98.44

0.515
  pilF Neisseria gonorrhoeae MS11

49.825

99.133

0.494
  pilB Vibrio cholerae strain A1552

50.452

95.841

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

47.321

97.054

0.459
  pilB Vibrio campbellii strain DS40M4

46.316

98.787

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

36.378

100

0.393

Multiple sequence alignment