Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   AAA996_RS09795 Genome accession   NZ_AP028606
Coordinates   1897703..1898719 (-) Length   338 a.a.
NCBI ID   WP_078375398.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain Sep1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1892703..1903719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAA996_RS09750 - 1892780..1892911 (-) 132 WP_000768904.1 hypothetical protein -
  AAA996_RS09755 (TKY121773_18970) - 1892912..1894102 (-) 1191 WP_000167766.1 acetate kinase -
  AAA996_RS09760 (TKY121773_18980) comYH 1894153..1895106 (-) 954 WP_000345101.1 class I SAM-dependent methyltransferase Machinery gene
  AAA996_RS09765 - 1895167..1895761 (-) 595 Protein_1898 methyltransferase domain-containing protein -
  AAA996_RS09770 (TKY121773_19010) comGG/cglG 1895898..1896311 (-) 414 WP_000265624.1 competence type IV pilus minor pilin ComGG Machinery gene
  AAA996_RS09775 (TKY121773_19020) comGF/cglF 1896289..1896750 (-) 462 WP_000250542.1 competence type IV pilus minor pilin ComGF Machinery gene
  AAA996_RS09780 (TKY121773_19030) comGE/cglE 1896713..1897015 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  AAA996_RS09785 (TKY121773_19040) comGD/cglD 1896978..1897382 (-) 405 WP_000588013.1 competence type IV pilus minor pilin ComGD Machinery gene
  AAA996_RS09790 (TKY121773_19050) comGC/cglC 1897375..1897701 (-) 327 WP_000738626.1 comG operon protein ComGC Machinery gene
  AAA996_RS09795 (TKY121773_19060) comGB/cglB 1897703..1898719 (-) 1017 WP_078375398.1 competence type IV pilus assembly protein ComGB Machinery gene
  AAA996_RS09800 (TKY121773_19070) comGA/cglA/cilD 1898667..1899608 (-) 942 WP_000249567.1 competence type IV pilus ATPase ComGA Machinery gene
  AAA996_RS09805 (TKY121773_19080) - 1899684..1900049 (-) 366 WP_000286415.1 DUF1033 family protein -
  AAA996_RS09810 (TKY121773_19090) - 1900200..1901258 (-) 1059 WP_000649473.1 zinc-dependent alcohol dehydrogenase family protein -
  AAA996_RS09815 (TKY121773_19100) nagA 1901421..1902572 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38537.60 Da        Isoelectric Point: 9.6162

>NTDB_id=106235 AAA996_RS09795 WP_078375398.1 1897703..1898719(-) (comGB/cglB) [Streptococcus pneumoniae strain Sep1]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMIVFVSVGVLLALTFYKRSSKMRVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=106235 AAA996_RS09795 WP_078375398.1 1897703..1898719(-) (comGB/cglB) [Streptococcus pneumoniae strain Sep1]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCTTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCCAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCACAAATCTTT
CTAGGAATGATAGTTTTTGTTTCAGTAGGTGTCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGCGCGTCTT
TTCTATCTTAGCGCGCCTTCCTTTTATTGGAATCTTTGTGCAGACCTATTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCTATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

97.929

100

0.979
  comGB/cglB Streptococcus pneumoniae D39

97.929

100

0.979
  comGB/cglB Streptococcus pneumoniae R6

97.929

100

0.979
  comGB/cglB Streptococcus pneumoniae TIGR4

97.929

100

0.979
  comGB/cglB Streptococcus mitis NCTC 12261

95.562

100

0.956
  comGB/cglB Streptococcus mitis SK321

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

70.833

99.408

0.704
  comYB Streptococcus mutans UA140

58.307

94.379

0.55
  comYB Streptococcus mutans UA159

58.307

94.379

0.55
  comGB Lactococcus lactis subsp. cremoris KW2

50.299

98.817

0.497

Multiple sequence alignment