Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACGI6K_RS02075 Genome accession   NZ_CP171449
Coordinates   404065..406164 (+) Length   699 a.a.
NCBI ID   WP_376944181.1    Uniprot ID   -
Organism   Azorhizophilus paspali strain ATCC 23833     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 399065..411164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGI6K_RS02055 (ACGI6K_02055) pilM 401150..402214 (+) 1065 WP_376944189.1 pilus assembly protein PilM Machinery gene
  ACGI6K_RS02060 (ACGI6K_02060) - 402215..402874 (+) 660 WP_376944187.1 PilN domain-containing protein -
  ACGI6K_RS02065 (ACGI6K_02065) pilO 402871..403491 (+) 621 WP_376944185.1 type 4a pilus biogenesis protein PilO -
  ACGI6K_RS02070 (ACGI6K_02070) - 403488..404012 (+) 525 WP_376944183.1 pilus assembly protein PilP -
  ACGI6K_RS02075 (ACGI6K_02075) pilQ 404065..406164 (+) 2100 WP_376944181.1 type IV pilus secretin PilQ Machinery gene
  ACGI6K_RS02080 (ACGI6K_02080) aroK 406169..406687 (+) 519 WP_376944179.1 shikimate kinase AroK -
  ACGI6K_RS02085 (ACGI6K_02085) aroB 406739..407842 (+) 1104 WP_376944177.1 3-dehydroquinate synthase -
  ACGI6K_RS02090 (ACGI6K_02090) - 407850..409448 (+) 1599 WP_376944175.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75612.83 Da        Isoelectric Point: 4.7690

>NTDB_id=1061243 ACGI6K_RS02075 WP_376944181.1 404065..406164(+) (pilQ) [Azorhizophilus paspali strain ATCC 23833]
MNSALSRFGISVLTALLSAKLTAADLESLDVAALPGDRVELKLSFDEAVPAPRGYTIDQPARIALDLPGVTNKLGTKHRE
LGVGNARSVTLVKASGRTQMIVNLAAMSPYSMRSEGHDLYVLIGESAAVARQSRSASPAVTQAKAYVTTGKVVRGIDFQR
GEQGEGNVIIDLSSPGMNPDIQEQGGKIRLDFPETQLPESLQARLDVKDFATPVHFVSASSQAGKASILIEPAGMYDYLV
YQADKRLTVNVKAKPQDSGAGRDTGRFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNLPWDQA
LDLVLRTKGLDKRKLGNVLLVAPADEIAARERQELEAQQRIAELAPLRRELLQVNYAKATDIAKLFQSVTSSDEVEEQRG
SITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEASVDYDKSLGVRWGGSFVNDQWSLYGKDGAQSVDD
GQVFLPGTGQVGNFTLENGMAPVPFVDMGVANSTSGLGIGFISDHLILDLQLSAMEKTGNGEVISQPKVLTSDKETAKIL
TGEEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDQADFAKALNGVPPINENEVNAKVLIGDGETIV
IGGVFSNIQAKSVDKVPFLGDLPLIGRLFRRDVVADSKSELLVFLTPRILNSQAIAVSR

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=1061243 ACGI6K_RS02075 WP_376944181.1 404065..406164(+) (pilQ) [Azorhizophilus paspali strain ATCC 23833]
ATGAACAGCGCCCTTTCGCGTTTCGGTATTTCGGTCCTGACCGCCCTGCTGTCGGCGAAATTGACGGCGGCCGATCTCGA
GTCCCTGGATGTGGCGGCCTTGCCCGGCGATCGGGTCGAACTCAAGCTGTCATTCGACGAGGCCGTGCCGGCACCACGTG
GCTACACGATCGACCAGCCGGCCCGGATTGCCCTGGATCTTCCCGGAGTGACCAACAAGCTGGGGACCAAGCACCGCGAG
CTGGGTGTCGGCAACGCCCGCAGCGTCACCCTGGTCAAGGCGAGCGGGCGTACTCAAATGATCGTCAACCTGGCCGCGAT
GTCGCCCTACAGTATGCGGAGCGAGGGCCATGACCTTTATGTGCTGATCGGCGAGTCTGCCGCTGTGGCCCGGCAGTCCC
GCAGTGCCTCGCCCGCGGTCACGCAAGCCAAGGCCTACGTGACGACGGGCAAGGTGGTTCGGGGCATCGACTTCCAGCGT
GGCGAGCAGGGCGAAGGCAACGTCATCATCGATCTGTCGAGCCCGGGCATGAATCCCGACATTCAGGAGCAGGGTGGCAA
GATCCGCCTGGACTTTCCGGAAACGCAATTGCCGGAGTCCCTGCAGGCACGCCTGGACGTCAAGGATTTCGCTACTCCCG
TGCACTTCGTGAGCGCCTCGAGCCAGGCCGGCAAGGCCAGCATCCTCATCGAGCCGGCCGGCATGTATGACTATCTGGTC
TACCAGGCCGACAAGCGACTGACCGTCAATGTCAAGGCGAAGCCCCAGGACAGCGGCGCCGGGCGTGATACCGGTCGCTT
CGCGTATACTGGCGAGAAGCTGTCGCTGAATTTCCAGGATATCGATGTCCGTTCGGTCCTGCAGTTGATCGCCGATTTCA
CCGATCTCAACTTGGTGGCCAGCGACACGGTACAGGGCAATATCACCCTTCGCCTGCAGAATTTGCCCTGGGATCAGGCG
CTCGACCTGGTGCTCAGAACCAAGGGCTTGGACAAGCGCAAGCTCGGCAACGTGCTGTTGGTGGCGCCGGCCGACGAAAT
CGCGGCACGTGAGCGCCAGGAGTTGGAGGCACAGCAGCGGATCGCCGAACTGGCTCCCCTGCGTCGCGAATTGCTCCAGG
TGAACTACGCCAAGGCGACGGACATCGCCAAACTGTTCCAGTCGGTCACCAGCAGCGATGAGGTGGAAGAACAGCGCGGC
TCCATCACCGTCGACGAGCGGACCAACAATATCATCGCCTACCAGACTCAAGACCGTCTCGACGAACTGCGGCGGATCGT
CGCCCAACTGGACATCCCGGTGCGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAGCGTCGATTACGACAAGAGCC
TGGGGGTGCGCTGGGGCGGAAGCTTCGTCAACGACCAGTGGAGCCTGTACGGCAAGGATGGCGCGCAATCCGTCGATGAC
GGGCAGGTATTTCTGCCGGGAACCGGCCAGGTCGGCAACTTCACCCTGGAGAATGGCATGGCTCCCGTTCCCTTCGTCGA
CATGGGGGTTGCGAACAGTACTTCGGGTCTCGGCATCGGCTTCATTTCCGATCACCTGATCCTGGATCTCCAACTGTCGG
CCATGGAAAAGACCGGCAATGGGGAAGTCATTTCCCAGCCCAAGGTTCTCACCTCGGACAAGGAGACGGCGAAGATCCTG
ACCGGCGAGGAGATTCCCTACCAGGAAGCTAGCTCCAGCGGCGCGACCAGCACTTCCTTCAAGGAGGCGGCCTTGTCGCT
GGAGGTGACGCCACAAATCACTCCTGACAACCGCATCATCATGGAGGTGAAGGTGACCAAGGATCAGGCGGACTTCGCCA
AGGCCCTGAACGGTGTGCCGCCGATCAACGAGAACGAGGTCAACGCCAAGGTGCTGATCGGCGACGGCGAAACCATCGTG
ATCGGCGGAGTTTTCTCCAATATCCAGGCCAAGTCCGTGGACAAGGTGCCGTTCCTAGGCGACCTGCCGTTGATCGGGCG
CCTGTTCCGCCGCGACGTGGTCGCCGACAGCAAATCCGAGCTGCTTGTTTTCTTGACTCCGCGTATTCTTAACAGCCAGG
CCATCGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(371-436)

AMIN domain (AMIN)

(37-121)

Bacterial type II and III secretion system protein (Secretin)

(534-690)

Secretin and TonB N terminus short domain (STN)

(297-343)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.849

100

0.734
  pilQ Acinetobacter baumannii strain A118

39.095

100

0.408
  pilQ Acinetobacter baumannii D1279779

39.095

100

0.408
  comQ Acinetobacter baylyi ADP1

39.884

98.999

0.395
  pilQ Legionella pneumophila strain ERS1305867

37.593

96.28

0.362
  pilQ Legionella pneumophila strain Lp02

37.444

96.28

0.361

Multiple sequence alignment