Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACE1MM_RS11550 Genome accession   NZ_CP171355
Coordinates   2432260..2432754 (-) Length   164 a.a.
NCBI ID   WP_062739026.1    Uniprot ID   -
Organism   Ralstonia mannitolilytica strain SY001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2427260..2437754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE1MM_RS11530 (ACE1MM_11530) - 2427442..2429151 (-) 1710 WP_062739024.1 M48 family metalloprotease -
  ACE1MM_RS11535 (ACE1MM_11535) moaC 2429258..2429734 (+) 477 WP_045786050.1 cyclic pyranopterin monophosphate synthase MoaC -
  ACE1MM_RS11540 (ACE1MM_11540) - 2429779..2430288 (-) 510 WP_316872147.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACE1MM_RS11545 (ACE1MM_11545) - 2430443..2432182 (-) 1740 WP_062739025.1 PglL family O-oligosaccharyltransferase -
  ACE1MM_RS11550 (ACE1MM_11550) pilA 2432260..2432754 (-) 495 WP_062739026.1 pilin Machinery gene
  ACE1MM_RS11555 (ACE1MM_11555) pilA 2433002..2433526 (-) 525 WP_062739049.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACE1MM_RS11560 (ACE1MM_11560) - 2433710..2434405 (-) 696 WP_062739027.1 TerC family protein -
  ACE1MM_RS11565 (ACE1MM_11565) sucD 2434575..2435456 (-) 882 WP_045786045.1 succinate--CoA ligase subunit alpha -
  ACE1MM_RS11570 (ACE1MM_11570) sucC 2435548..2436714 (-) 1167 WP_062739028.1 ADP-forming succinate--CoA ligase subunit beta -
  ACE1MM_RS11575 (ACE1MM_11575) - 2436781..2437407 (-) 627 WP_062739050.1 DUF2889 domain-containing protein -

Sequence

Protein


Download         Length: 164 a.a.        Molecular weight: 16903.40 Da        Isoelectric Point: 10.1347

>NTDB_id=1060841 ACE1MM_RS11550 WP_062739026.1 2432260..2432754(-) (pilA) [Ralstonia mannitolilytica strain SY001]
MKSMRRLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTIRARVTEGLSLAAAAKVVVADNAANATGDLSNGYTQPS
GMKNVSSISIASNTGAISIAYNSNVAASGANVVTLTPLDGTSAVSATTPPTNPIKWVCQTANSTSVTNVQKGTLASKYAP
AECR

Nucleotide


Download         Length: 495 bp        

>NTDB_id=1060841 ACE1MM_RS11550 WP_062739026.1 2432260..2432754(-) (pilA) [Ralstonia mannitolilytica strain SY001]
ATGAAGTCGATGCGTCGTCTGAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTTTGGCTGCTATCGCCATTCCGGCTTATCAGGACTACACGATCCGTGCGCGTGTGACGGAAGGGTTGTCGTTGG
CTGCAGCGGCCAAGGTAGTCGTGGCAGACAATGCTGCCAATGCTACGGGAGATCTGTCCAATGGCTATACTCAGCCGAGC
GGCATGAAAAACGTGAGCAGCATCTCTATTGCTTCGAATACCGGCGCGATTTCCATCGCATACAATAGCAACGTGGCTGC
TTCGGGCGCTAACGTGGTTACCTTGACGCCGCTGGATGGCACGAGTGCTGTGTCCGCGACCACACCGCCCACCAATCCTA
TCAAGTGGGTTTGCCAAACGGCCAATTCCACAAGCGTCACTAACGTCCAGAAGGGCACTCTGGCCAGCAAGTACGCACCG
GCTGAGTGCCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)

Pilin (bacterial filament) (Pilin)

(44-164)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

56.14

100

0.585
  pilA2 Legionella pneumophila str. Paris

49.367

96.341

0.476
  pilA2 Legionella pneumophila strain ERS1305867

49.367

96.341

0.476
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.842

100

0.427
  comP Acinetobacter baylyi ADP1

42.405

96.341

0.409

Multiple sequence alignment