Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACE1MM_RS11555 Genome accession   NZ_CP171355
Coordinates   2433002..2433526 (-) Length   174 a.a.
NCBI ID   WP_062739049.1    Uniprot ID   A0AAJ5D3U2
Organism   Ralstonia mannitolilytica strain SY001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2428002..2438526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE1MM_RS11535 (ACE1MM_11535) moaC 2429258..2429734 (+) 477 WP_045786050.1 cyclic pyranopterin monophosphate synthase MoaC -
  ACE1MM_RS11540 (ACE1MM_11540) - 2429779..2430288 (-) 510 WP_316872147.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACE1MM_RS11545 (ACE1MM_11545) - 2430443..2432182 (-) 1740 WP_062739025.1 PglL family O-oligosaccharyltransferase -
  ACE1MM_RS11550 (ACE1MM_11550) pilA 2432260..2432754 (-) 495 WP_062739026.1 pilin Machinery gene
  ACE1MM_RS11555 (ACE1MM_11555) pilA 2433002..2433526 (-) 525 WP_062739049.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACE1MM_RS11560 (ACE1MM_11560) - 2433710..2434405 (-) 696 WP_062739027.1 TerC family protein -
  ACE1MM_RS11565 (ACE1MM_11565) sucD 2434575..2435456 (-) 882 WP_045786045.1 succinate--CoA ligase subunit alpha -
  ACE1MM_RS11570 (ACE1MM_11570) sucC 2435548..2436714 (-) 1167 WP_062739028.1 ADP-forming succinate--CoA ligase subunit beta -
  ACE1MM_RS11575 (ACE1MM_11575) - 2436781..2437407 (-) 627 WP_062739050.1 DUF2889 domain-containing protein -
  ACE1MM_RS11580 (ACE1MM_11580) recX 2437696..2438175 (-) 480 WP_045786043.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 174 a.a.        Molecular weight: 18287.05 Da        Isoelectric Point: 9.1225

>NTDB_id=1060842 ACE1MM_RS11555 WP_062739049.1 2433002..2433526(-) (pilA) [Ralstonia mannitolilytica strain SY001]
MGFKHCKGTRRRSDDGFTLIELMIVVAIVGILAAIAIPAYQDYTIRSRVTEGLIVAVQAKALVVENAANSQSDLSLGWAS
LPIGKNVSGMQVNSGNGEITIQFSSAVAQAGSNQLILTPYSGAASSTYVNLSASAPPPTLVMWACSAKGKQMPLNVTRTA
TPTLEARYAPAECR

Nucleotide


Download         Length: 525 bp        

>NTDB_id=1060842 ACE1MM_RS11555 WP_062739049.1 2433002..2433526(-) (pilA) [Ralstonia mannitolilytica strain SY001]
ATGGGCTTCAAGCACTGCAAGGGAACAAGGCGCCGGTCTGACGACGGCTTTACCTTGATCGAATTGATGATTGTCGTGGC
GATCGTGGGGATTCTCGCGGCGATTGCGATCCCGGCGTATCAGGACTATACGATTCGGTCGCGGGTGACTGAAGGATTGA
TTGTCGCGGTTCAAGCCAAGGCTTTGGTGGTTGAGAACGCCGCCAACTCACAGAGCGATCTATCGCTGGGATGGGCCTCG
CTGCCTATTGGCAAGAACGTCTCCGGCATGCAAGTGAATAGCGGGAACGGCGAGATCACGATCCAGTTCTCATCAGCGGT
GGCCCAAGCGGGGTCGAATCAGTTGATCCTTACGCCGTATTCGGGCGCGGCTTCGTCCACGTACGTCAACCTGAGCGCCA
GCGCACCACCTCCGACGCTGGTGATGTGGGCGTGTTCGGCGAAGGGCAAGCAGATGCCGCTCAACGTCACCCGGACTGCC
ACGCCAACGCTCGAGGCTCGATACGCGCCTGCCGAATGCCGCTAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(46-174)

Prokaryotic N-terminal methylation motif (N_methyl)

(11-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

50.568

100

0.511
  pilA2 Legionella pneumophila strain ERS1305867

42.945

93.678

0.402
  pilA2 Legionella pneumophila str. Paris

42.331

93.678

0.397
  comP Acinetobacter baylyi ADP1

38.65

93.678

0.362

Multiple sequence alignment