Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACF6ZU_RS14680 Genome accession   NZ_CP171224
Coordinates   3155334..3157421 (+) Length   695 a.a.
NCBI ID   WP_392893555.1    Uniprot ID   -
Organism   Pseudomonas migulae strain HY-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3150334..3162421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF6ZU_RS14660 (ACF6ZU_14660) comM 3152546..3153610 (+) 1065 WP_392893548.1 pilus assembly protein PilM Machinery gene
  ACF6ZU_RS14665 (ACF6ZU_14665) - 3153610..3154176 (+) 567 WP_392893549.1 PilN domain-containing protein -
  ACF6ZU_RS14670 (ACF6ZU_14670) pilO 3154173..3154796 (+) 624 WP_392893551.1 type 4a pilus biogenesis protein PilO -
  ACF6ZU_RS14675 (ACF6ZU_14675) - 3154793..3155320 (+) 528 WP_392893553.1 pilus assembly protein PilP -
  ACF6ZU_RS14680 (ACF6ZU_14680) pilQ 3155334..3157421 (+) 2088 WP_392893555.1 type IV pilus secretin PilQ Machinery gene
  ACF6ZU_RS14685 (ACF6ZU_14685) aroK 3157426..3157944 (+) 519 WP_392893557.1 shikimate kinase AroK -
  ACF6ZU_RS14690 (ACF6ZU_14690) aroB 3158095..3159195 (+) 1101 WP_017336126.1 3-dehydroquinate synthase -
  ACF6ZU_RS14695 (ACF6ZU_14695) - 3159206..3160807 (+) 1602 WP_392893558.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75180.36 Da        Isoelectric Point: 5.7416

>NTDB_id=1060549 ACF6ZU_RS14680 WP_392893555.1 3155334..3157421(+) (pilQ) [Pseudomonas migulae strain HY-2]
MNRIFATFGISLWIALLSPMVHAANLKALDVAALPGDRIELKLSFDGAPPAPHGYTTEQPARIALDLPGVVSQLASKTRD
LGGGNARSATVVEAKDRTRLIINLTQLTPYTARVEGNNLFVVVGRGSSNAASAPAAKPSRVATATPAPAKAYAPVSKSIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGRIVLGFARTQLPEPLRVRLDVKDFATPVQFVNASATGDRATISIEPSG
AFDYSTYQTDNKLTVSIRPMTVDDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVS
NGANASSTIGTPGSTSTNSPFVDMGTASNTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=1060549 ACF6ZU_RS14680 WP_392893555.1 3155334..3157421(+) (pilQ) [Pseudomonas migulae strain HY-2]
ATGAACAGGATTTTCGCAACCTTCGGAATTTCGCTATGGATAGCGTTGCTGTCACCGATGGTGCACGCGGCCAATCTCAA
AGCGCTGGATGTCGCTGCGCTTCCGGGGGACCGCATCGAGTTGAAGCTGTCATTCGATGGCGCGCCGCCGGCACCCCATG
GCTACACGACGGAGCAACCTGCACGCATTGCCCTGGACCTGCCCGGCGTCGTCAGTCAGCTGGCAAGCAAGACCCGCGAC
CTGGGCGGCGGCAATGCCCGCAGCGCTACGGTGGTGGAGGCCAAGGACCGCACGCGACTGATCATCAACCTGACGCAATT
GACCCCGTACACGGCTCGCGTCGAAGGCAACAATCTGTTTGTCGTGGTCGGGCGTGGATCGAGTAACGCCGCTTCTGCAC
CGGCCGCCAAGCCGTCGCGTGTCGCCACGGCAACCCCGGCACCGGCCAAGGCGTATGCGCCGGTGAGCAAGTCGATTCGC
GGCGTGGATTTCCAGCGCGGCACCCAGGGTGAAGGCAATGTGGTCATCGATCTGTCGGACCCGTCCATCGCCCCGGACAT
TCAGGAACGCGATGGCCGGATTGTCCTCGGCTTCGCCAGGACCCAATTGCCTGAACCGCTTCGGGTGCGCCTGGACGTCA
AGGACTTCGCCACGCCCGTGCAATTCGTCAACGCCAGTGCCACCGGGGATCGGGCGACCATCAGCATCGAGCCCAGCGGC
GCGTTCGACTACTCGACCTATCAGACCGACAACAAGCTGACCGTCAGCATCCGCCCGATGACTGTCGATGACTTGCAGAA
ACGCAACGCCGAGCGGTTTGCCTACACCGGTGAAAAGCTCTCGCTGAATTTCCAGGACATCGACGTGCGCTCGGTACTGC
AACTGATCGCCGACTTCACCAACCTCAATCTCGTGGCCAGTGACACAGTGCAGGGCGGCATCACCCTGCGCCTGCAGAAC
GTGCCCTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAAGGTCTGGACAAACGCAAGATCGGCAACGTGTTGCTGGT
GGCGCCGGCCGATGAAATTGCCGCGCGCGAGCGCCAGGAACTGGAGTCGCAGAAACAGATTGCCGAACTCGCGCCCCTGC
GTCGCGAGCTGCTGCAGGTCAATTACGCCAAGGCCGCCGATATCGCCAAGTTGTTCCAGTCGGTGACCAGTGCCGAAGCG
AAAGTCGATGAGCGGGGGTCGATCACTGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
CGAGCTTCGTCGTATCGTCGCGCAACTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAAGCCAACG
TCGACTACGACAAAAGCCTTGGCGTGCGCTGGGGCGGATCGATACAGAACAAGGGCAACTGGAACACCTCCGGAGTCAGC
AATGGTGCCAATGCCTCGTCGACCATCGGCACGCCCGGCAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGCACTGC
CTCCAACACCTCGGGGCTCGGTATCGCGTTCATCACCGACAATGTGCTGCTGGATCTCGAGCTGACCGCGATGGAGAAGA
CCGGCAACGGCGAAATCGTCTCGCAGCCCAAGGTGGTCACGTCCGACAAGGAAACCGCGAAAATCCTCAAGGGCACCGAG
ATTCCCTATCAGGAAGCCAGCTCCAGCGGCGCGACTTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAAGTGACGCC
GCAGATCACCCCGGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTCAACAAAGTGCAGG
ATGTACCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTATTGGTCAATGACGGCGAGACCATCGTGATCGGTGGCGTT
TTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGACGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTGTTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGA
GTCATTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Secretin and TonB N terminus short domain (STN)

(303-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.147

100

0.753
  comQ Acinetobacter baylyi ADP1

41.774

100

0.42
  pilQ Acinetobacter baumannii strain A118

38.38

100

0.416
  pilQ Acinetobacter baumannii D1279779

38.38

100

0.416
  pilQ Legionella pneumophila strain Lp02

37.885

97.986

0.371
  pilQ Legionella pneumophila strain ERS1305867

37.885

97.986

0.371

Multiple sequence alignment