Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   ACF6ZU_RS14660 Genome accession   NZ_CP171224
Coordinates   3152546..3153610 (+) Length   354 a.a.
NCBI ID   WP_392893548.1    Uniprot ID   -
Organism   Pseudomonas migulae strain HY-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3147546..3158610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF6ZU_RS14650 (ACF6ZU_14650) - 3148381..3149649 (+) 1269 WP_392893546.1 malic enzyme-like NAD(P)-binding protein -
  ACF6ZU_RS14655 (ACF6ZU_14655) - 3149890..3152325 (-) 2436 WP_392893823.1 penicillin-binding protein 1A -
  ACF6ZU_RS14660 (ACF6ZU_14660) comM 3152546..3153610 (+) 1065 WP_392893548.1 pilus assembly protein PilM Machinery gene
  ACF6ZU_RS14665 (ACF6ZU_14665) - 3153610..3154176 (+) 567 WP_392893549.1 PilN domain-containing protein -
  ACF6ZU_RS14670 (ACF6ZU_14670) pilO 3154173..3154796 (+) 624 WP_392893551.1 type 4a pilus biogenesis protein PilO -
  ACF6ZU_RS14675 (ACF6ZU_14675) - 3154793..3155320 (+) 528 WP_392893553.1 pilus assembly protein PilP -
  ACF6ZU_RS14680 (ACF6ZU_14680) pilQ 3155334..3157421 (+) 2088 WP_392893555.1 type IV pilus secretin PilQ Machinery gene
  ACF6ZU_RS14685 (ACF6ZU_14685) aroK 3157426..3157944 (+) 519 WP_392893557.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38013.40 Da        Isoelectric Point: 4.7361

>NTDB_id=1060548 ACF6ZU_RS14660 WP_392893548.1 3152546..3153610(+) (comM) [Pseudomonas migulae strain HY-2]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPASAVVEKNIAELEGVGQALTRVLLKARTSLRNVAV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGASARNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLTGHLAAAQEQLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTT
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSDMTLSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1060548 ACF6ZU_RS14660 WP_392893548.1 3152546..3153610(+) (comM) [Pseudomonas migulae strain HY-2]
GTGCTAGGACTCTTCAATAAAAAGGCCAATACGCTGTTGGGGATCGACATCAGCTCCACGTCGGTAAAGCTTCTGGAGCT
GAGCCGACAGGGCGACCGCTACCGGGTCGAGGCCTATGCGGTAGAGCCGCTGCCGGCGAGCGCGGTGGTCGAGAAGAATA
TCGCCGAGCTCGAAGGCGTGGGCCAGGCACTCACGCGCGTGTTGCTCAAGGCCCGGACCAGTCTGCGGAACGTCGCCGTG
GCCGTGGCCGGTTCCGCGGTGATCACCAAGACCATCGAAATGGACGCCGGTCTTTCCGACGATGAGCTGGAAAACCAGCT
GAAGATCGAAGCCGATCAATACATCCCTTATCCGCTGGACGAAGTGGCCATCGACTTCGAAGTCCAGGGCGCGTCGGCAC
GCAACCCCGAGCGGGTCAATGTGCTGCTGGCGGCCTGTCGCAAGGAAAACGTCGAAGTCCGCGAAGCGGCCCTGGCGCTC
GCCGGCCTGACCGCGCGCGTGGTCGATGTCGAGGCCTACGCGTTGGAGCGTTCGTTCGGTTTGCTCACCGGGCATCTGGC
CGCGGCCCAGGAGCAACTGACCGTCGCCGTGGTCGACATCGGCGCGACCATGACCACCCTGAGCGTGCTGCACAACGGCA
AGATCATCTACACCCGGGAACAGTTGTTCGGCGGTCGCCAGTTGACGGAAGAAATCCAGCGTCGTTACGGCCTGACGACC
GAGCAGGCGGGGCTGGCGAAGAAACAGGGCGGCCTGCCGGATGATTACGTCAGCGAAGTGTTGCAGCCGTTTCGCGATGC
GCTGGTGCAGCAAGTGTCGCGTTCACTGCAGTTTTTCTTTGCCTCGGGTCAGTACAACGCGGTCGATCACATCCTGCTGG
CCGGCGGTACCGCCTCGGTTCCGGGCCTGGATCGCTTGATCGAGCAGCGACTGGGCACGCCGACGCAAGTGGCCAATCCG
TTTTCGGACATGACCCTGAGCAGCAAGGTCAACGCCGGTGCGCTGGCCAGTGACGCACCCGCGCTGATGATCGCCTGCGG
ACTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

54.802

100

0.548
  pilM Legionella pneumophila strain ERS1305867

48.023

100

0.48

Multiple sequence alignment