Detailed information NTG1060469

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACF0HW_RS06255
• Genome accession: NZ_CP171208
• Coordinates: 1217596..1218210 (+)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus amyloliquefaciens strain HN11
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1212596..1223210

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF0HW_RS06220	aroE	1213284..1214126 (+)	843	WP_012118031.1	shikimate dehydrogenase	-
ACF0HW_RS06225	yhbY	1214120..1214410 (+)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
ACF0HW_RS06230	-	1214421..1214990 (+)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
ACF0HW_RS06235	yqeK	1214980..1215540 (+)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACF0HW_RS06240	rsfS	1215557..1215913 (+)	357	WP_007408260.1	ribosome silencing factor	-
ACF0HW_RS06245	-	1215910..1216647 (+)	738	WP_012118028.1	class I SAM-dependent methyltransferase	-
ACF0HW_RS06250	comER	1216716..1217537 (-)	822	WP_012118027.1	late competence protein ComER	-
ACF0HW_RS06255	comEA	1217596..1218210 (+)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACF0HW_RS06260	-	1218277..1218846 (+)	570	WP_003152868.1	ComE operon protein 2	-
ACF0HW_RS06265	comEC	1218847..1221198 (+)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACF0HW_RS06270	-	1221217..1221351 (-)	135	WP_003152870.1	YqzM family protein	-
ACF0HW_RS06275	-	1221392..1221543 (+)	152	Protein_1224	hypothetical protein	-
ACF0HW_RS06280	holA	1221583..1222624 (+)	1042	Protein_1225	DNA polymerase III subunit delta	-
ACF0HW_RS06285	rpsT	1222641..1222907 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=1060469 ACF0HW_RS06255 WP_012118026.1 1217596..1218210(+) (comEA) [Bacillus amyloliquefaciens strain HN11]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1060469 ACF0HW_RS06255 WP_012118026.1 1217596..1218210(+) (comEA) [Bacillus amyloliquefaciens strain HN11]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363