Detailed information NTG1060471

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: ACF0HW_RS06265
• Genome accession: NZ_CP171208
• Coordinates: 1218847..1221198 (+)
• Length: 783 a.a.
• NCBI ID: WP_012118025.1
• Uniprot ID: A7Z6X1
• Organism: Bacillus amyloliquefaciens strain HN11
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1213847..1226198

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF0HW_RS06225	yhbY	1214120..1214410 (+)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
ACF0HW_RS06230	-	1214421..1214990 (+)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
ACF0HW_RS06235	yqeK	1214980..1215540 (+)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACF0HW_RS06240	rsfS	1215557..1215913 (+)	357	WP_007408260.1	ribosome silencing factor	-
ACF0HW_RS06245	-	1215910..1216647 (+)	738	WP_012118028.1	class I SAM-dependent methyltransferase	-
ACF0HW_RS06250	comER	1216716..1217537 (-)	822	WP_012118027.1	late competence protein ComER	-
ACF0HW_RS06255	comEA	1217596..1218210 (+)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACF0HW_RS06260	-	1218277..1218846 (+)	570	WP_003152868.1	ComE operon protein 2	-
ACF0HW_RS06265	comEC	1218847..1221198 (+)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACF0HW_RS06270	-	1221217..1221351 (-)	135	WP_003152870.1	YqzM family protein	-
ACF0HW_RS06275	-	1221392..1221543 (+)	152	Protein_1224	hypothetical protein	-
ACF0HW_RS06280	holA	1221583..1222624 (+)	1042	Protein_1225	DNA polymerase III subunit delta	-
ACF0HW_RS06285	rpsT	1222641..1222907 (-)	267	WP_003152876.1	30S ribosomal protein S20	-
ACF0HW_RS06290	gpr	1223110..1224216 (+)	1107	WP_007408268.1	GPR endopeptidase	-
ACF0HW_RS06295	spoIIP	1224284..1225477 (+)	1194	WP_007612673.1	stage II sporulation protein P	-
ACF0HW_RS06300	-	1225494..1225832 (+)	339	WP_007408270.1	YqxA family protein	-

Sequence

Protein

• Length: 783 a.a.
• Molecular weight: 86682.54 Da
• Isoelectric Point: 9.0968

>NTDB_id=1060471 ACF0HW_RS06265 WP_012118025.1 1218847..1221198(+) (comEC) [Bacillus amyloliquefaciens strain HN11]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIIVKTKQYAPVIVCLVSFCVYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLYSQQI
HWLFSVTSIQQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFGMFDLVMKPVHDFITYAASVDLFTMIVSKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVFGGVSWLLTGDLESDGETEVLKTYPNLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFKKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide

• Length: 2352 bp

>NTDB_id=1060471 ACF0HW_RS06265 WP_012118025.1 1218847..1221198(+) (comEC) [Bacillus amyloliquefaciens strain HN11]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATTGTAAAAACAAAGCAGTATGCTCCGGTTATCGTCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGCTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGGTCTCTTGAGGAAAAGAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACATTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAAGCAGGAATTTTGCTGCTGCT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCCCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTTTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCCTCATTCATTGCAGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAGATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTCTCAAGGCAGATGGGAGAATGTTTGTTTGGTATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCATTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCACTGATTTTAACCCATGCGGATCAAGATCACATCGGGGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGAGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTTGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACAGAAGTGCTGAAAACGTATCCGAATCTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAGCAGCTTCAGCCGGAAGCGGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTAAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Competence protein (Competence) (208-469)

• Domain of unknown function (DUF4131) (DUF4131) (25-171)

• Metallo-beta-lactamase superfamily (Lactamase_B) (508-719)

3D structure

• AlphaFold DB: A7Z6X1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	56.995	98.595	0.562