Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACF8MK_RS00055 Genome accession   NZ_CP171154
Coordinates   11276..12340 (-) Length   354 a.a.
NCBI ID   WP_392537653.1    Uniprot ID   -
Organism   Legionella sp. 227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6276..17340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF8MK_RS00035 (ACF8MK_00035) pilQ 7445..9550 (-) 2106 WP_392537649.1 type IV pilus secretin PilQ Machinery gene
  ACF8MK_RS00040 (ACF8MK_00040) pilP 9559..10089 (-) 531 WP_392537650.1 pilus assembly protein PilP Machinery gene
  ACF8MK_RS00045 (ACF8MK_00045) pilO 10086..10685 (-) 600 WP_392537651.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACF8MK_RS00050 (ACF8MK_00050) pilN 10682..11269 (-) 588 WP_392537652.1 PilN domain-containing protein Machinery gene
  ACF8MK_RS00055 (ACF8MK_00055) pilM 11276..12340 (-) 1065 WP_392537653.1 pilus assembly protein PilM Machinery gene
  ACF8MK_RS00060 (ACF8MK_00060) - 12584..13672 (+) 1089 WP_392537654.1 hypothetical protein -
  ACF8MK_RS00065 (ACF8MK_00065) - 13864..16230 (+) 2367 WP_392537655.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39161.14 Da        Isoelectric Point: 4.6023

>NTDB_id=1060322 ACF8MK_RS00055 WP_392537653.1 11276..12340(-) (pilM) [Legionella sp. 227]
MLKLFQPKHRSILGIDITSTSVKVLEISGQEGALVVENYGREVLPSNAMDGNTIKDIDVVSRCIKKVIDRLHTSCKQAAL
AVPDASIISKVIQINEGLNDEEMEELIVTEADKYIPYPIDEINLDFEILGQSEKNPHMLDVLIVASRAENVNQRVETAVR
AGLDAIVIDVESYAVERAVQQLAKDLPASGQDKTIAIIDVGASYTHLFVLQKMKLVYSREEKFGGKQLIESIAEHYKMTL
EQAMLAKESGELPEDYEKEVLEPFKEDILLQIKRTLQFFYSTSQDGDVDHILLAGGLAKLPGLVSLVQERLGMSTTIANP
FSYMTPGKMMNLDAINNDAPSLMVACGLALRNIK

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1060322 ACF8MK_RS00055 WP_392537653.1 11276..12340(-) (pilM) [Legionella sp. 227]
ATGCTCAAATTGTTTCAGCCCAAACATCGTTCAATTCTCGGAATAGACATTACATCAACGTCGGTAAAAGTATTGGAAAT
TTCAGGCCAAGAAGGTGCGCTTGTTGTAGAAAACTATGGACGCGAAGTATTACCATCAAATGCCATGGATGGGAACACAA
TTAAGGATATTGACGTAGTTTCCCGTTGTATTAAGAAAGTAATCGATCGCTTGCATACATCATGTAAGCAGGCTGCTTTA
GCCGTTCCTGATGCTTCAATAATTAGCAAGGTCATACAAATCAACGAGGGTTTAAATGATGAGGAAATGGAGGAACTCAT
TGTTACTGAGGCTGATAAATACATTCCCTATCCAATCGATGAAATCAATCTTGATTTTGAAATTTTAGGCCAGTCTGAAA
AAAATCCGCATATGTTAGATGTTCTTATTGTTGCATCACGAGCGGAAAATGTGAATCAGCGTGTTGAGACTGCGGTTCGT
GCAGGCTTAGATGCAATAGTGATTGACGTAGAGTCTTATGCTGTGGAACGAGCGGTTCAACAGCTTGCTAAAGATTTACC
TGCTTCAGGACAAGATAAAACAATAGCGATCATTGATGTTGGTGCTAGTTATACGCATTTATTTGTATTACAGAAAATGA
AATTAGTGTACTCCAGGGAGGAAAAATTTGGGGGTAAACAATTAATAGAGTCGATCGCGGAACATTATAAAATGACTTTA
GAACAAGCAATGCTCGCTAAAGAGAGTGGAGAATTACCTGAAGACTACGAAAAGGAAGTTTTAGAACCTTTTAAAGAGGA
TATATTGTTGCAAATCAAGCGTACCTTGCAGTTTTTTTATTCTACAAGTCAAGATGGGGATGTGGATCATATACTTTTGG
CTGGAGGATTAGCCAAACTTCCAGGCTTGGTCTCATTGGTTCAAGAGCGATTAGGAATGAGTACAACTATTGCGAATCCA
TTTTCATATATGACTCCAGGTAAAATGATGAACTTGGATGCGATCAATAATGATGCTCCATCACTTATGGTCGCTTGTGG
CCTGGCATTAAGGAATATTAAGTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

69.774

100

0.698
  pilM Acinetobacter baumannii D1279779

44.759

99.718

0.446
  comM Acinetobacter nosocomialis M2

44.759

99.718

0.446
  comM Acinetobacter baylyi ADP1

43.626

99.718

0.435

Multiple sequence alignment