Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACF8MK_RS00050 Genome accession   NZ_CP171154
Coordinates   10682..11269 (-) Length   195 a.a.
NCBI ID   WP_392537652.1    Uniprot ID   -
Organism   Legionella sp. 227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5682..16269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF8MK_RS00030 (ACF8MK_00030) aroK 6240..6767 (-) 528 WP_010653139.1 shikimate kinase AroK -
  ACF8MK_RS00035 (ACF8MK_00035) pilQ 7445..9550 (-) 2106 WP_392537649.1 type IV pilus secretin PilQ Machinery gene
  ACF8MK_RS00040 (ACF8MK_00040) pilP 9559..10089 (-) 531 WP_392537650.1 pilus assembly protein PilP Machinery gene
  ACF8MK_RS00045 (ACF8MK_00045) pilO 10086..10685 (-) 600 WP_392537651.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACF8MK_RS00050 (ACF8MK_00050) pilN 10682..11269 (-) 588 WP_392537652.1 PilN domain-containing protein Machinery gene
  ACF8MK_RS00055 (ACF8MK_00055) pilM 11276..12340 (-) 1065 WP_392537653.1 pilus assembly protein PilM Machinery gene
  ACF8MK_RS00060 (ACF8MK_00060) - 12584..13672 (+) 1089 WP_392537654.1 hypothetical protein -
  ACF8MK_RS00065 (ACF8MK_00065) - 13864..16230 (+) 2367 WP_392537655.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 195 a.a.        Molecular weight: 22627.32 Da        Isoelectric Point: 6.2565

>NTDB_id=1060321 ACF8MK_RS00050 WP_392537652.1 10682..11269(-) (pilN) [Legionella sp. 227]
MTQINLLPWRELKREQEKKLFTIMLLVCVVAAAFIVFLINSYASGLVSNQITRNQMLQKEINAMDAQLKEIKNLEKTREM
LISRMSIVQHLQSTRTLMVHLFDELINITPSGVYLTQVEGKNDIISVLGYTESNTFVSILMRNIENNDWLHNPVLSEIKR
EENDKDKDKEKQQSTENEFKLTFVLAPQFQIGIIL

Nucleotide


Download         Length: 588 bp        

>NTDB_id=1060321 ACF8MK_RS00050 WP_392537652.1 10682..11269(-) (pilN) [Legionella sp. 227]
ATGACGCAGATTAACCTTCTTCCCTGGCGAGAGTTAAAGCGGGAGCAGGAAAAAAAACTGTTTACGATCATGCTGCTTGT
GTGCGTTGTGGCTGCTGCTTTTATTGTATTTTTAATTAACTCCTATGCTTCGGGATTGGTGAGTAATCAAATTACGCGAA
ATCAAATGCTTCAAAAAGAAATTAATGCGATGGATGCACAATTAAAAGAAATTAAAAATTTGGAAAAAACTAGAGAAATG
CTCATTTCCAGAATGTCTATAGTACAGCATTTACAATCTACTCGAACATTAATGGTTCATTTATTTGATGAGCTAATTAA
TATTACGCCATCCGGGGTTTATCTTACCCAGGTGGAAGGCAAAAATGATATCATTTCAGTCCTAGGATATACGGAGTCGA
ATACATTTGTTTCTATATTAATGAGAAACATTGAAAATAATGATTGGCTCCATAATCCTGTCCTTAGTGAAATCAAGAGG
GAAGAAAACGATAAAGATAAGGATAAAGAGAAACAGCAATCTACTGAAAATGAATTTAAATTAACTTTTGTACTTGCCCC
TCAATTCCAGATTGGAATCATATTATGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-183)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

66.138

96.923

0.641

Multiple sequence alignment