Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACF8MK_RS00035 Genome accession   NZ_CP171154
Coordinates   7445..9550 (-) Length   701 a.a.
NCBI ID   WP_392537649.1    Uniprot ID   -
Organism   Legionella sp. 227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2445..14550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF8MK_RS00015 (ACF8MK_00015) proC 2630..3466 (+) 837 WP_392537646.1 pyrroline-5-carboxylate reductase -
  ACF8MK_RS00020 (ACF8MK_00020) - 3708..5147 (-) 1440 WP_392537647.1 SPOR domain-containing protein -
  ACF8MK_RS00025 (ACF8MK_00025) aroB 5150..6253 (-) 1104 WP_392537648.1 3-dehydroquinate synthase -
  ACF8MK_RS00030 (ACF8MK_00030) aroK 6240..6767 (-) 528 WP_010653139.1 shikimate kinase AroK -
  ACF8MK_RS00035 (ACF8MK_00035) pilQ 7445..9550 (-) 2106 WP_392537649.1 type IV pilus secretin PilQ Machinery gene
  ACF8MK_RS00040 (ACF8MK_00040) pilP 9559..10089 (-) 531 WP_392537650.1 pilus assembly protein PilP Machinery gene
  ACF8MK_RS00045 (ACF8MK_00045) pilO 10086..10685 (-) 600 WP_392537651.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACF8MK_RS00050 (ACF8MK_00050) pilN 10682..11269 (-) 588 WP_392537652.1 PilN domain-containing protein Machinery gene
  ACF8MK_RS00055 (ACF8MK_00055) pilM 11276..12340 (-) 1065 WP_392537653.1 pilus assembly protein PilM Machinery gene
  ACF8MK_RS00060 (ACF8MK_00060) - 12584..13672 (+) 1089 WP_392537654.1 hypothetical protein -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 77620.92 Da        Isoelectric Point: 9.7319

>NTDB_id=1060318 ACF8MK_RS00035 WP_392537649.1 7445..9550(-) (pilQ) [Legionella sp. 227]
MRRITALLIFIGMSLCTMAFAQNNPLESVKVIPLPEEKLRLDFQFAKPLKQEPASFVTQKPARIVLDFIDSNLQLPPEQR
TKEIKLGSLNNYSIVAVGSRVRAILDLNFPVPYSGSISGKVYTLILNGKSNELFQPPKEILITNKPVKTSYEINHFDFRG
IERQGGRAIVNVSNSSVPIEVEEHGKEILVKFLNTNIPQNLRKRFDVADFRSPVQLVTFQQAGKNAHMILSNKGNFGQYV
YQINKQFMIDVFPLTQEEAQQAKLKKKVFSGRRISLNFQNISIRAVLQLLADFTGINIVVSDKVQGNITLRLNNIPWDQA
LDIILTTQGLAKRQTGNVMLIDYKASFDKMEAEELKSQHIIQKLEPVRSELIQINYAKATDLAILIKDKQNSLLSEKGKV
SVDTRTNTIWIQDSGTKIDEVRDLIKQLDVPVKQVLIEARIVEVTKDFAQDLGIRWGVSKPPHLSGTLAGANQMEQGVAP
ANVVPFTDRLNLDLVAAPVTGAVPASVGIALAQLGDNILLDLELSALESEGLAELISSPRLITANQQPAVIDSGQEIPYQ
EATSSGATAVAFKKAVLSLKVTPQITPDSKILMELKINQDTASPQLFNGVPAILTKEIQTNVLVSNGQTIVLGGIYQQDK
SKTITRVPFFGQLPVVGNLFKNTQIALKNDELLIFITPKIITNSLSITTIEGRKPIRFDEK

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=1060318 ACF8MK_RS00035 WP_392537649.1 7445..9550(-) (pilQ) [Legionella sp. 227]
GTGCGGAGAATAACTGCTTTATTGATTTTTATTGGCATGAGCTTATGTACGATGGCTTTTGCCCAAAATAATCCTCTTGA
ATCGGTAAAGGTCATTCCTTTGCCTGAGGAAAAGTTGCGTTTGGATTTCCAATTTGCGAAACCTCTTAAGCAAGAACCTG
CAAGTTTTGTGACCCAAAAACCGGCACGTATCGTTCTAGATTTTATTGATTCCAACCTGCAATTACCTCCCGAGCAGAGG
ACGAAAGAAATTAAATTAGGTTCACTAAATAACTACAGCATTGTTGCCGTGGGGTCTCGTGTTCGAGCAATATTGGATTT
AAATTTCCCTGTACCTTATTCCGGTTCAATTTCTGGAAAAGTTTATACACTGATTTTGAATGGTAAAAGCAACGAGTTAT
TTCAACCTCCTAAAGAAATTTTAATCACCAATAAACCGGTCAAGACAAGTTATGAAATCAATCATTTCGACTTTCGTGGG
ATTGAGAGACAAGGTGGCCGTGCAATAGTCAATGTTTCCAATAGCAGCGTTCCAATTGAGGTCGAAGAACATGGTAAAGA
AATTCTAGTGAAATTTTTAAATACAAATATTCCACAGAATCTCAGGAAACGCTTTGATGTTGCCGATTTTCGCAGCCCAG
TGCAACTCGTTACTTTTCAGCAAGCTGGTAAAAACGCGCACATGATTTTGTCTAACAAGGGTAATTTTGGTCAATATGTT
TATCAAATTAATAAACAATTCATGATTGATGTCTTTCCCTTAACTCAAGAAGAAGCGCAACAGGCTAAATTGAAAAAGAA
GGTATTTTCTGGAAGAAGAATTTCTTTGAATTTTCAGAATATCAGCATACGCGCTGTTTTACAGTTATTGGCTGATTTTA
CTGGAATTAATATTGTCGTAAGCGATAAAGTTCAAGGAAATATCACTCTAAGATTAAATAACATTCCCTGGGATCAAGCC
TTAGATATTATTTTAACCACGCAAGGACTCGCTAAGCGTCAGACGGGTAATGTAATGTTGATCGATTATAAGGCGTCTTT
CGATAAAATGGAGGCAGAAGAGCTTAAAAGTCAGCATATTATTCAGAAACTTGAACCAGTACGTTCTGAACTTATTCAGA
TTAATTATGCAAAAGCTACTGATCTTGCGATTTTAATTAAAGACAAACAAAATTCACTTCTATCTGAAAAGGGTAAAGTA
AGTGTTGATACACGAACAAATACCATCTGGATTCAAGACAGCGGCACCAAAATCGATGAAGTCAGGGATTTGATAAAACA
GTTAGATGTACCTGTTAAACAAGTGCTTATTGAGGCACGTATTGTCGAAGTCACTAAAGATTTTGCGCAAGACTTGGGAA
TACGATGGGGTGTTTCCAAACCGCCTCATTTAAGTGGTACTTTAGCTGGAGCGAACCAAATGGAGCAGGGGGTTGCTCCT
GCAAATGTCGTACCGTTTACAGACCGATTAAACTTGGATTTGGTTGCTGCTCCTGTGACGGGAGCAGTCCCTGCCTCTGT
TGGTATTGCATTGGCTCAACTGGGCGATAATATTTTACTGGATCTTGAGTTATCCGCTTTGGAAAGTGAGGGGCTTGCTG
AATTGATATCCAGTCCGAGACTTATTACTGCGAATCAACAGCCTGCAGTAATTGATTCAGGACAGGAGATACCTTACCAG
GAGGCCACTTCAAGTGGTGCGACTGCAGTAGCGTTCAAAAAAGCGGTATTAAGTTTAAAGGTAACTCCGCAAATTACTCC
TGATAGTAAAATACTTATGGAGTTAAAAATTAATCAGGATACGGCTTCACCTCAATTATTTAACGGTGTGCCTGCCATTT
TAACTAAGGAAATTCAAACTAATGTCCTTGTTAGTAATGGACAGACTATAGTTCTAGGGGGAATCTATCAGCAAGACAAG
AGTAAAACGATTACTAGAGTACCTTTTTTTGGTCAGCTGCCTGTAGTCGGAAATTTATTTAAAAATACCCAAATTGCTCT
TAAAAACGATGAGTTACTTATTTTTATTACGCCAAAAATAATAACCAATTCATTATCGATTACGACAATTGAAGGCAGAA
AACCGATTCGTTTTGATGAAAAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(526-681)

Secretin and TonB N terminus short domain (STN)

(296-341)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain Lp02

74.742

96.576

0.722
  pilQ Legionella pneumophila strain ERS1305867

74.667

96.291

0.719
  pilQ Pseudomonas aeruginosa PAK

36.022

100

0.369
  comQ Acinetobacter baylyi ADP1

37.069

99.287

0.368

Multiple sequence alignment