Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACF8MK_RS00045 Genome accession   NZ_CP171154
Coordinates   10086..10685 (-) Length   199 a.a.
NCBI ID   WP_392537651.1    Uniprot ID   -
Organism   Legionella sp. 227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5086..15685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF8MK_RS00025 (ACF8MK_00025) aroB 5150..6253 (-) 1104 WP_392537648.1 3-dehydroquinate synthase -
  ACF8MK_RS00030 (ACF8MK_00030) aroK 6240..6767 (-) 528 WP_010653139.1 shikimate kinase AroK -
  ACF8MK_RS00035 (ACF8MK_00035) pilQ 7445..9550 (-) 2106 WP_392537649.1 type IV pilus secretin PilQ Machinery gene
  ACF8MK_RS00040 (ACF8MK_00040) pilP 9559..10089 (-) 531 WP_392537650.1 pilus assembly protein PilP Machinery gene
  ACF8MK_RS00045 (ACF8MK_00045) pilO 10086..10685 (-) 600 WP_392537651.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACF8MK_RS00050 (ACF8MK_00050) pilN 10682..11269 (-) 588 WP_392537652.1 PilN domain-containing protein Machinery gene
  ACF8MK_RS00055 (ACF8MK_00055) pilM 11276..12340 (-) 1065 WP_392537653.1 pilus assembly protein PilM Machinery gene
  ACF8MK_RS00060 (ACF8MK_00060) - 12584..13672 (+) 1089 WP_392537654.1 hypothetical protein -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 23062.75 Da        Isoelectric Point: 4.9179

>NTDB_id=1060320 ACF8MK_RS00045 WP_392537651.1 10086..10685(-) (pilO) [Legionella sp. 227]
MNNVNLNELTLENVGQWPLVVKICVFLGVSFLIIALGYWLIIQDNFTQYSKLEGQEATLKTDFENKQRQLYNLPAFREQL
QIMSERFVLMLKQLPEKNEMPGLLEEISKTGVASGLRFELFAPQPEVMHDFYVELPIKISVVGTYFQLAMFISRVAEMNR
IVTLHDFTIEGVSSKDHKTVSEDELEMSITAKIYRYRTQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=1060320 ACF8MK_RS00045 WP_392537651.1 10086..10685(-) (pilO) [Legionella sp. 227]
ATGAACAACGTCAACCTAAATGAACTAACTCTGGAAAATGTGGGACAATGGCCATTGGTGGTCAAAATATGTGTTTTTTT
GGGTGTTAGTTTTCTAATTATTGCTTTAGGATATTGGTTGATCATTCAAGATAATTTCACACAGTACAGTAAATTGGAAG
GTCAGGAAGCAACTTTAAAAACAGATTTTGAAAACAAGCAACGGCAATTATATAATTTACCTGCCTTTCGTGAACAACTG
CAGATTATGAGTGAGCGTTTTGTACTAATGCTCAAGCAGCTTCCTGAAAAAAATGAGATGCCTGGCTTGTTGGAAGAAAT
TTCTAAAACAGGGGTGGCCTCAGGATTAAGGTTTGAGTTATTTGCCCCGCAACCAGAAGTAATGCATGATTTTTATGTTG
AATTACCTATAAAGATTTCAGTGGTAGGGACTTATTTTCAATTGGCTATGTTTATAAGTCGAGTGGCTGAAATGAATCGA
ATTGTAACGTTACATGATTTTACTATTGAAGGGGTGTCTTCCAAGGATCATAAAACGGTCTCGGAAGATGAGTTGGAAAT
GAGCATTACTGCTAAAATATATCGATATCGCACACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(51-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

74.372

100

0.744
  comO Acinetobacter baylyi ADP1

40.314

95.98

0.387
  pilO Acinetobacter baumannii D1279779

39.267

95.98

0.377

Multiple sequence alignment