Detailed information NTG1058825

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACFXAC_RS12245
• Genome accession: NZ_CP170720
• Coordinates: 2544417..2545031 (-)
• Length: 204 a.a.
• NCBI ID: WP_053573180.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain JP5006
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2539417..2550031

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFXAC_RS12215 (ACFXAC_12215)	rpsT	2539717..2539983 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACFXAC_RS12220 (ACFXAC_12220)	holA	2540000..2541041 (-)	1042	Protein_2366	DNA polymerase III subunit delta	-
ACFXAC_RS12225 (ACFXAC_12225)	-	2541081..2541235 (-)	155	Protein_2367	hypothetical protein	-
ACFXAC_RS12230 (ACFXAC_12230)	-	2541276..2541410 (+)	135	WP_003152870.1	YqzM family protein	-
ACFXAC_RS12235 (ACFXAC_12235)	comEC	2541429..2543780 (-)	2352	WP_053573181.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACFXAC_RS12240 (ACFXAC_12240)	-	2543781..2544350 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACFXAC_RS12245 (ACFXAC_12245)	comEA	2544417..2545031 (-)	615	WP_053573180.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACFXAC_RS12250 (ACFXAC_12250)	comER	2545090..2545911 (+)	822	WP_015417855.1	late competence protein ComER	-
ACFXAC_RS12255 (ACFXAC_12255)	-	2545980..2546717 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
ACFXAC_RS12260 (ACFXAC_12260)	rsfS	2546714..2547070 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACFXAC_RS12265 (ACFXAC_12265)	yqeK	2547088..2547648 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACFXAC_RS12270 (ACFXAC_12270)	-	2547638..2548207 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACFXAC_RS12275 (ACFXAC_12275)	yhbY	2548218..2548508 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACFXAC_RS12280 (ACFXAC_12280)	aroE	2548502..2549344 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22183.34 Da
• Isoelectric Point: 6.4888

>NTDB_id=1058825 ACFXAC_RS12245 WP_053573180.1 2544417..2545031(-) (comEA) [Bacillus amyloliquefaciens strain JP5006]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTMVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1058825 ACFXAC_RS12245 WP_053573180.1 2544417..2545031(-) (comEA) [Bacillus amyloliquefaciens strain JP5006]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTTGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATGGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363