Detailed information NTG1058823

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: ACFXAC_RS12235
• Genome accession: NZ_CP170720
• Coordinates: 2541429..2543780 (-)
• Length: 783 a.a.
• NCBI ID: WP_053573181.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain JP5006
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2536429..2548780

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFXAC_RS12200 (ACFXAC_12200)	-	2536792..2537130 (-)	339	WP_007408270.1	YqxA family protein	-
ACFXAC_RS12205 (ACFXAC_12205)	spoIIP	2537147..2538340 (-)	1194	WP_015417852.1	stage II sporulation protein P	-
ACFXAC_RS12210 (ACFXAC_12210)	gpr	2538408..2539514 (-)	1107	WP_053573183.1	GPR endopeptidase	-
ACFXAC_RS12215 (ACFXAC_12215)	rpsT	2539717..2539983 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACFXAC_RS12220 (ACFXAC_12220)	holA	2540000..2541041 (-)	1042	Protein_2366	DNA polymerase III subunit delta	-
ACFXAC_RS12225 (ACFXAC_12225)	-	2541081..2541235 (-)	155	Protein_2367	hypothetical protein	-
ACFXAC_RS12230 (ACFXAC_12230)	-	2541276..2541410 (+)	135	WP_003152870.1	YqzM family protein	-
ACFXAC_RS12235 (ACFXAC_12235)	comEC	2541429..2543780 (-)	2352	WP_053573181.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACFXAC_RS12240 (ACFXAC_12240)	-	2543781..2544350 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACFXAC_RS12245 (ACFXAC_12245)	comEA	2544417..2545031 (-)	615	WP_053573180.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACFXAC_RS12250 (ACFXAC_12250)	comER	2545090..2545911 (+)	822	WP_015417855.1	late competence protein ComER	-
ACFXAC_RS12255 (ACFXAC_12255)	-	2545980..2546717 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
ACFXAC_RS12260 (ACFXAC_12260)	rsfS	2546714..2547070 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACFXAC_RS12265 (ACFXAC_12265)	yqeK	2547088..2547648 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACFXAC_RS12270 (ACFXAC_12270)	-	2547638..2548207 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACFXAC_RS12275 (ACFXAC_12275)	yhbY	2548218..2548508 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-

Sequence

Protein

• Length: 783 a.a.
• Molecular weight: 86668.43 Da
• Isoelectric Point: 8.6744

>NTDB_id=1058823 ACFXAC_RS12235 WP_053573181.1 2541429..2543780(-) (comEC) [Bacillus amyloliquefaciens strain JP5006]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIIVKTKHHAPVIVCLVSFCVYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLYSQQI
HWLFSVTSIEQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGESRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFGMFDLVMKPVHDFITYAASVDLFTMIVSKPDFVSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVFGGVSWLLTGDLESDGETEVLKTYPKLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFEKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide

• Length: 2352 bp

>NTDB_id=1058823 ACFXAC_RS12235 WP_053573181.1 2541429..2543780(-) (comEC) [Bacillus amyloliquefaciens strain JP5006]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCTGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATTGTAAAAACAAAGCACCATGCTCCGGTTATCGTCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGCTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATTACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAGTG
GGCGGCTTCGTACAAAATCCGTTCTCTTGAGGAAAAAAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTGAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGAATTTTGCTGCTGCT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCCTTGTCTTATCTGCTGCTTCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGGAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAGATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCTTTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTCTCAAGGCAGATGGGAGAATGTTTGTTTGGTATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTGTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCATATTCTATCGGCGAGAAGGTTTTGATTCCCTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGGGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTAGGATTCGTAAAAGAACCGAAAGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGGGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTTGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAAACTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTGAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Domain of unknown function (DUF4131) (DUF4131) (25-171)

• Competence protein (Competence) (208-469)

• Metallo-beta-lactamase superfamily (Lactamase_B) (508-719)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	56.865	98.595	0.561