Detailed information NTG0105873

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: DK42_RS00440
• Genome accession: NZ_CP007571
• Coordinates: 70121..71119 (+)
• Length: 332 a.a.
• NCBI ID: WP_000196633.1
• Uniprot ID: Q3K3X8
• Organism: Streptococcus agalactiae strain GBS2-NM
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 65121..76119

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DK42_RS00415 (DK42_00415)	-	65931..66302 (+)	372	WP_001872566.1	hypothetical protein	-
DK42_RS00420 (DK42_00420)	-	66269..66673 (+)	405	WP_223297811.1	DUF6261 family protein	-
DK42_RS00425 (DK42_00425)	-	66673..67482 (+)	810	WP_000982340.1	hypothetical protein	-
DK42_RS00430 (DK42_00430)	purB	67479..68777 (+)	1299	WP_000572889.1	adenylosuccinate lyase	-
DK42_RS00435 (DK42_00435)	comR	68930..69832 (+)	903	WP_000912098.1	helix-turn-helix domain-containing protein	Regulator
DK42_RS00440 (DK42_00440)	ruvB	70121..71119 (+)	999	WP_000196633.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
DK42_RS00445 (DK42_00445)	-	71271..71708 (+)	438	WP_000754818.1	low molecular weight protein-tyrosine-phosphatase	-
DK42_RS00450 (DK42_00450)	-	71715..72095 (+)	381	WP_000787702.1	membrane protein	-
DK42_RS00455 (DK42_00455)	-	72092..73870 (+)	1779	WP_001220904.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37573.90 Da
• Isoelectric Point: 4.5053

>NTDB_id=105873 DK42_RS00440 WP_000196633.1 70121..71119(+) (ruvB) [Streptococcus agalactiae strain GBS2-NM]
MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEAAKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAIEKSGDLVAILNDLEPGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPRIANRLLKRVRDYAQIMGDGLID
DNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGGPVGLGTLSVNIAEERDTVEDMYEPYLIQKGFIMRTRTGRVATDK
AYEHLGYQRFDK

Nucleotide

• Length: 999 bp

>NTDB_id=105873 DK42_RS00440 WP_000196633.1 70121..71119(+) (ruvB) [Streptococcus agalactiae strain GBS2-NM]
ATGACAAGATTTTTAGATAGTGATGCAATGGGTGACGAAGAATTGGTAGAACGTACACTTCGTCCGCAGTATTTAAGAGA
GTATATTGGACAAGATAAGGTTAAAGATCAGCTAAAAATATTTATTGAAGCTGCTAAATTGCGTGATGAGTCATTGGATC
ATGTGTTATTATTTGGCCCTCCTGGTTTAGGGAAAACAACCATGGCATTTGTAATTGCTAATGAGTTGGGTGTCAATCTC
AAACAAACATCAGGTCCTGCAATTGAAAAATCAGGGGATTTAGTAGCCATTTTAAATGATTTAGAACCAGGTGATGTTCT
TTTTATTGATGAAATTCATCGTATGCCGATGGCGGTTGAAGAGGTGCTCTATAGTGCAATGGAAGACTTTTATATTGACA
TTATGATCGGTGCAGGAGAAACTAGTAGAAGTGTTCATCTAGATTTGCCGCCCTTTACCTTAATTGGTGCAACGACACGT
GCAGGTATGTTATCTAATCCCTTACGTGCTCGCTTTGGTATTACAGGGCATATGGAGTATTATGAAGAAAATGATTTGAC
AGAAATTATTGAGCGTACAGCAGACATTTTTGAAATGAAAATTACTTATGAAGCTGCTTCTGAATTAGCGCGTCGCAGTC
GTGGAACGCCACGTATCGCTAACCGTTTATTGAAACGTGTTCGAGATTATGCTCAAATCATGGGAGATGGTTTGATAGAT
GACAATATTACAGATAAAGCATTAACGATGTTAGATGTTGATCACGAGGGGCTTGATTACGTCGATCAAAAAATCTTAAG
AACCATGATTGAAATGTATAATGGAGGTCCTGTTGGTTTAGGAACTCTATCCGTTAATATTGCTGAAGAACGAGATACTG
TTGAAGACATGTACGAACCTTATTTAATTCAAAAAGGTTTTATTATGCGTACCCGTACCGGTCGTGTAGCTACGGATAAG
GCATACGAACATTTAGGTTATCAGCGATTTGATAAATAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

3D structure

• AlphaFold DB: Q3K3X8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae TIGR4	90.06	100	0.901
ruvB	Streptococcus pneumoniae R6	89.759	100	0.898
ruvB	Streptococcus pneumoniae D39	89.759	100	0.898
ruvB	Bacillus subtilis subsp. subtilis str. 168	59.819	99.699	0.596
ruvB	Helicobacter pylori 26695	53.074	93.072	0.494
ruvB	Synechocystis sp. PCC 6803	50.479	94.277	0.476