Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   ACFW99_RS12255 Genome accession   NZ_CP170651
Coordinates   2535783..2536091 (-) Length   102 a.a.
NCBI ID   WP_015417818.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2530783..2541091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS12205 (ACFW99_12205) sinR 2530947..2531282 (+) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  ACFW99_RS12210 (ACFW99_12210) tasA 2531330..2532115 (-) 786 WP_007408329.1 biofilm matrix protein TasA -
  ACFW99_RS12215 (ACFW99_12215) sipW 2532180..2532764 (-) 585 WP_061860711.1 signal peptidase I SipW -
  ACFW99_RS12220 (ACFW99_12220) tapA 2532736..2533407 (-) 672 WP_060674605.1 amyloid fiber anchoring/assembly protein TapA -
  ACFW99_RS12225 (ACFW99_12225) - 2533666..2533995 (+) 330 WP_060674607.1 DUF3889 domain-containing protein -
  ACFW99_RS12230 (ACFW99_12230) - 2534035..2534214 (-) 180 WP_003153093.1 YqzE family protein -
  ACFW99_RS12235 (ACFW99_12235) comGG 2534271..2534648 (-) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACFW99_RS12240 (ACFW99_12240) comGF 2534649..2535149 (-) 501 WP_254922226.1 competence type IV pilus minor pilin ComGF -
  ACFW99_RS12245 (ACFW99_12245) comGE 2535058..2535372 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  ACFW99_RS12250 (ACFW99_12250) comGD 2535356..2535793 (-) 438 WP_043020787.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACFW99_RS12255 (ACFW99_12255) comGC 2535783..2536091 (-) 309 WP_015417818.1 competence type IV pilus major pilin ComGC Machinery gene
  ACFW99_RS12260 (ACFW99_12260) comGB 2536096..2537133 (-) 1038 WP_015417819.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACFW99_RS12265 (ACFW99_12265) comGA 2537120..2538190 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  ACFW99_RS12270 (ACFW99_12270) - 2538383..2539333 (-) 951 WP_015417820.1 magnesium transporter CorA family protein -
  ACFW99_RS12275 (ACFW99_12275) - 2539479..2540780 (+) 1302 WP_012117986.1 hemolysin family protein -

Sequence

Protein


Download         Length: 102 a.a.        Molecular weight: 11409.33 Da        Isoelectric Point: 6.2096

>NTDB_id=1058419 ACFW99_RS12255 WP_015417818.1 2535783..2536091(-) (comGC) [Bacillus amyloliquefaciens strain JP5025]
MLRLKNQDGFTLIEMLIVLFIVSILLLITIPNVTKHNQSIQHKGCEGLQNMVKAQVTAYEIDHEGKMPDMNDLQSEGYIK
KNTVCPNGKQILISGGEVTVEQ

Nucleotide


Download         Length: 309 bp        

>NTDB_id=1058419 ACFW99_RS12255 WP_015417818.1 2535783..2536091(-) (comGC) [Bacillus amyloliquefaciens strain JP5025]
GTGCTTCGTCTGAAAAATCAAGATGGATTTACTTTAATTGAAATGCTGATTGTTTTATTTATCGTTTCCATTCTGCTTTT
AATCACCATTCCTAACGTTACAAAACATAATCAAAGCATTCAGCATAAAGGCTGTGAAGGACTGCAGAATATGGTGAAAG
CCCAAGTGACGGCGTACGAAATCGACCATGAAGGTAAAATGCCGGATATGAACGATTTACAATCAGAGGGGTATATCAAA
AAGAATACAGTCTGCCCGAATGGTAAACAGATCTTAATTAGTGGCGGAGAAGTTACAGTTGAACAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

78.125

94.118

0.735
  comGC Staphylococcus aureus MW2

42.708

94.118

0.402
  comGC Staphylococcus aureus N315

42.708

94.118

0.402

Multiple sequence alignment