Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACFW99_RS12265 Genome accession   NZ_CP170651
Coordinates   2537120..2538190 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2532120..2543190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS12215 (ACFW99_12215) sipW 2532180..2532764 (-) 585 WP_061860711.1 signal peptidase I SipW -
  ACFW99_RS12220 (ACFW99_12220) tapA 2532736..2533407 (-) 672 WP_060674605.1 amyloid fiber anchoring/assembly protein TapA -
  ACFW99_RS12225 (ACFW99_12225) - 2533666..2533995 (+) 330 WP_060674607.1 DUF3889 domain-containing protein -
  ACFW99_RS12230 (ACFW99_12230) - 2534035..2534214 (-) 180 WP_003153093.1 YqzE family protein -
  ACFW99_RS12235 (ACFW99_12235) comGG 2534271..2534648 (-) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACFW99_RS12240 (ACFW99_12240) comGF 2534649..2535149 (-) 501 WP_254922226.1 competence type IV pilus minor pilin ComGF -
  ACFW99_RS12245 (ACFW99_12245) comGE 2535058..2535372 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  ACFW99_RS12250 (ACFW99_12250) comGD 2535356..2535793 (-) 438 WP_043020787.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACFW99_RS12255 (ACFW99_12255) comGC 2535783..2536091 (-) 309 WP_015417818.1 competence type IV pilus major pilin ComGC Machinery gene
  ACFW99_RS12260 (ACFW99_12260) comGB 2536096..2537133 (-) 1038 WP_015417819.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACFW99_RS12265 (ACFW99_12265) comGA 2537120..2538190 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  ACFW99_RS12270 (ACFW99_12270) - 2538383..2539333 (-) 951 WP_015417820.1 magnesium transporter CorA family protein -
  ACFW99_RS12275 (ACFW99_12275) - 2539479..2540780 (+) 1302 WP_012117986.1 hemolysin family protein -
  ACFW99_RS12280 (ACFW99_12280) - 2540818..2541648 (-) 831 WP_015417821.1 STAS domain-containing protein -
  ACFW99_RS12290 (ACFW99_12290) - 2541855..2542235 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACFW99_RS12295 (ACFW99_12295) - 2542472..2542717 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=1058421 ACFW99_RS12265 WP_003153083.1 2537120..2538190(-) (comGA) [Bacillus amyloliquefaciens strain JP5025]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1058421 ACFW99_RS12265 WP_003153083.1 2537120..2538190(-) (comGA) [Bacillus amyloliquefaciens strain JP5025]
GTGAAAAATATTGAAACATATAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATTTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTATA
GGCAGACACGCCGTGCGGGTATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment