Detailed information
Bioinformatically predicted
Overview
| Name | comGB | Type | Machinery gene |
| Locus tag | ACFW99_RS12260 | Genome accession | NZ_CP170651 |
| Coordinates | 2536096..2537133 (-) | Length | 345 a.a. |
| NCBI ID | WP_015417819.1 | Uniprot ID | - |
| Organism | Bacillus amyloliquefaciens strain JP5025 | ||
| Function | dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake |
||
Genomic Context
Location: 2531096..2542133
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ACFW99_RS12210 (ACFW99_12210) | tasA | 2531330..2532115 (-) | 786 | WP_007408329.1 | biofilm matrix protein TasA | - |
| ACFW99_RS12215 (ACFW99_12215) | sipW | 2532180..2532764 (-) | 585 | WP_061860711.1 | signal peptidase I SipW | - |
| ACFW99_RS12220 (ACFW99_12220) | tapA | 2532736..2533407 (-) | 672 | WP_060674605.1 | amyloid fiber anchoring/assembly protein TapA | - |
| ACFW99_RS12225 (ACFW99_12225) | - | 2533666..2533995 (+) | 330 | WP_060674607.1 | DUF3889 domain-containing protein | - |
| ACFW99_RS12230 (ACFW99_12230) | - | 2534035..2534214 (-) | 180 | WP_003153093.1 | YqzE family protein | - |
| ACFW99_RS12235 (ACFW99_12235) | comGG | 2534271..2534648 (-) | 378 | WP_015417814.1 | competence type IV pilus minor pilin ComGG | Machinery gene |
| ACFW99_RS12240 (ACFW99_12240) | comGF | 2534649..2535149 (-) | 501 | WP_254922226.1 | competence type IV pilus minor pilin ComGF | - |
| ACFW99_RS12245 (ACFW99_12245) | comGE | 2535058..2535372 (-) | 315 | WP_012117982.1 | competence type IV pilus minor pilin ComGE | - |
| ACFW99_RS12250 (ACFW99_12250) | comGD | 2535356..2535793 (-) | 438 | WP_043020787.1 | competence type IV pilus minor pilin ComGD | Machinery gene |
| ACFW99_RS12255 (ACFW99_12255) | comGC | 2535783..2536091 (-) | 309 | WP_015417818.1 | competence type IV pilus major pilin ComGC | Machinery gene |
| ACFW99_RS12260 (ACFW99_12260) | comGB | 2536096..2537133 (-) | 1038 | WP_015417819.1 | competence type IV pilus assembly protein ComGB | Machinery gene |
| ACFW99_RS12265 (ACFW99_12265) | comGA | 2537120..2538190 (-) | 1071 | WP_003153083.1 | competence type IV pilus ATPase ComGA | Machinery gene |
| ACFW99_RS12270 (ACFW99_12270) | - | 2538383..2539333 (-) | 951 | WP_015417820.1 | magnesium transporter CorA family protein | - |
| ACFW99_RS12275 (ACFW99_12275) | - | 2539479..2540780 (+) | 1302 | WP_012117986.1 | hemolysin family protein | - |
| ACFW99_RS12280 (ACFW99_12280) | - | 2540818..2541648 (-) | 831 | WP_015417821.1 | STAS domain-containing protein | - |
Sequence
Protein
Download Length: 345 a.a. Molecular weight: 39689.06 Da Isoelectric Point: 9.5935
>NTDB_id=1058420 ACFW99_RS12260 WP_015417819.1 2536096..2537133(-) (comGB) [Bacillus amyloliquefaciens strain JP5025]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLQLQLHKRQLAELKEGIRRLREGDAFYQVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLIFSVCVMFYILQSLIIPQFSGIYQSMNMNTSGTTAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLQLQLHKRQLAELKEGIRRLREGDAFYQVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLIFSVCVMFYILQSLIIPQFSGIYQSMNMNTSGTTAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM
Nucleotide
Download Length: 1038 bp
>NTDB_id=1058420 ACFW99_RS12260 WP_015417819.1 2536096..2537133(-) (comGB) [Bacillus amyloliquefaciens strain JP5025]
ATGAAGCGGATTAAACGAACATGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGCCTGATTGAAGGGCTCAGACTGCTGCAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGAAGGAAGGAATCC
GCCGGCTGAGAGAAGGTGATGCGTTTTACCAAGTGCTTGAGGCACTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCGGAGAAACACGGGGAATTGCCGGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTTTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACGTCCGGTACGACTGCTTTCATCTTTGCGTTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTTTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAGTCCCCGCAAGATAAAATGCTGATTGTTGTGAAAATACCCCTCTTAGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGAGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
TGGGCATCCGTGTTATGAAAAAGACCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA
ATGAAGCGGATTAAACGAACATGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGCCTGATTGAAGGGCTCAGACTGCTGCAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGAAGGAAGGAATCC
GCCGGCTGAGAGAAGGTGATGCGTTTTACCAAGTGCTTGAGGCACTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCGGAGAAACACGGGGAATTGCCGGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTTTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACGTCCGGTACGACTGCTTTCATCTTTGCGTTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTTTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAGTCCCCGCAAGATAAAATGCTGATTGTTGTGAAAATACCCCTCTTAGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGAGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
TGGGCATCCGTGTTATGAAAAAGACCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| comGB | Bacillus subtilis subsp. subtilis str. 168 |
65.635 |
93.623 |
0.614 |