Detailed information    

insolico Bioinformatically predicted

Overview

Name   sinR   Type   Regulator
Locus tag   ACFW99_RS12205 Genome accession   NZ_CP170651
Coordinates   2530947..2531282 (+) Length   111 a.a.
NCBI ID   WP_003153104.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   repression of rok; repression of degU; repression of spo0A (predicted from homology)   
Competence regulation

Genomic Context

Location: 2525947..2536282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS12185 (ACFW99_12185) gcvT 2526553..2527653 (-) 1101 WP_031378949.1 glycine cleavage system aminomethyltransferase GcvT -
  ACFW99_RS12190 (ACFW99_12190) - 2528077..2529747 (+) 1671 WP_015417810.1 DEAD/DEAH box helicase -
  ACFW99_RS12195 (ACFW99_12195) - 2529769..2530563 (+) 795 WP_156240427.1 YqhG family protein -
  ACFW99_RS12200 (ACFW99_12200) sinI 2530740..2530913 (+) 174 WP_003153105.1 anti-repressor SinI Regulator
  ACFW99_RS12205 (ACFW99_12205) sinR 2530947..2531282 (+) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  ACFW99_RS12210 (ACFW99_12210) tasA 2531330..2532115 (-) 786 WP_007408329.1 biofilm matrix protein TasA -
  ACFW99_RS12215 (ACFW99_12215) sipW 2532180..2532764 (-) 585 WP_061860711.1 signal peptidase I SipW -
  ACFW99_RS12220 (ACFW99_12220) tapA 2532736..2533407 (-) 672 WP_060674605.1 amyloid fiber anchoring/assembly protein TapA -
  ACFW99_RS12225 (ACFW99_12225) - 2533666..2533995 (+) 330 WP_060674607.1 DUF3889 domain-containing protein -
  ACFW99_RS12230 (ACFW99_12230) - 2534035..2534214 (-) 180 WP_003153093.1 YqzE family protein -
  ACFW99_RS12235 (ACFW99_12235) comGG 2534271..2534648 (-) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACFW99_RS12240 (ACFW99_12240) comGF 2534649..2535149 (-) 501 WP_254922226.1 competence type IV pilus minor pilin ComGF -
  ACFW99_RS12245 (ACFW99_12245) comGE 2535058..2535372 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  ACFW99_RS12250 (ACFW99_12250) comGD 2535356..2535793 (-) 438 WP_043020787.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACFW99_RS12255 (ACFW99_12255) comGC 2535783..2536091 (-) 309 WP_015417818.1 competence type IV pilus major pilin ComGC Machinery gene

Sequence

Protein


Download         Length: 111 a.a.        Molecular weight: 12972.64 Da        Isoelectric Point: 7.2023

>NTDB_id=1058416 ACFW99_RS12205 WP_003153104.1 2530947..2531282(+) (sinR) [Bacillus amyloliquefaciens strain JP5025]
MIGQRIKQYRKEKGYSLSELAEKAGVAKSYLSSIERNLQTNPSIQFLEKVSAVLDVSVHTLLDEKHETEYDGQLDSEWEK
LVRDAMTSGVSKKQFREFLDYQKWRKAQKEE

Nucleotide


Download         Length: 336 bp        

>NTDB_id=1058416 ACFW99_RS12205 WP_003153104.1 2530947..2531282(+) (sinR) [Bacillus amyloliquefaciens strain JP5025]
TTGATTGGCCAGCGTATTAAACAATACCGAAAAGAAAAAGGCTACTCACTATCAGAACTAGCTGAAAAAGCTGGGGTAGC
GAAGTCTTATTTAAGCTCAATAGAACGAAATTTACAGACGAACCCCTCCATTCAATTTCTCGAAAAAGTCTCCGCTGTTC
TGGACGTCTCGGTTCATACTTTACTTGATGAAAAACATGAAACCGAATACGATGGTCAATTAGATAGTGAATGGGAGAAA
TTAGTTCGCGATGCGATGACATCCGGGGTATCAAAAAAACAATTTCGTGAATTTTTAGATTATCAAAAATGGAGAAAAGC
GCAAAAAGAGGAGTAG

Domains

Predicted by InterproScan.

Helix-turn-helix (HTH_3)

(6-58)

Anti-repressor SinI (SinI)

(74-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sinR Bacillus subtilis subsp. subtilis str. 168

99.099

100

0.991

Multiple sequence alignment