Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O1Q83_RS01770 Genome accession   NZ_CP170642
Coordinates   363049..364461 (+) Length   470 a.a.
NCBI ID   WP_132301910.1    Uniprot ID   A0A4R1KY20
Organism   Lonepinella koalarum strain ACM3666     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 358049..369461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1Q83_RS01740 (O1Q83_00347) - 359343..359564 (-) 222 WP_132301922.1 YejL family protein -
  O1Q83_RS01745 (O1Q83_00348) yejK 359673..360692 (+) 1020 WP_132301920.1 nucleoid-associated protein YejK -
  O1Q83_RS01750 (O1Q83_00349) bamE 360755..361171 (+) 417 WP_132301918.1 outer membrane protein assembly factor BamE -
  O1Q83_RS01755 (O1Q83_00350) - 361250..361900 (-) 651 WP_132301916.1 HAD family phosphatase -
  O1Q83_RS01760 (O1Q83_00351) ampD 361904..362449 (-) 546 WP_132301914.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  O1Q83_RS01765 (O1Q83_00352) pilA 362573..363046 (+) 474 WP_132301912.1 prepilin peptidase-dependent pilin Machinery gene
  O1Q83_RS01770 (O1Q83_00353) pilB 363049..364461 (+) 1413 WP_132301910.1 GspE/PulE family protein Machinery gene
  O1Q83_RS01775 (O1Q83_00354) pilC 364458..365678 (+) 1221 WP_132301908.1 type II secretion system F family protein Machinery gene
  O1Q83_RS01780 (O1Q83_00355) - 365675..366373 (+) 699 WP_132301906.1 A24 family peptidase -
  O1Q83_RS01785 (O1Q83_00356) coaE 366399..367028 (+) 630 WP_132301904.1 dephospho-CoA kinase -
  O1Q83_RS01790 (O1Q83_00357) yacG 367012..367212 (+) 201 WP_132301902.1 DNA gyrase inhibitor YacG -
  O1Q83_RS01795 (O1Q83_00358) - 367215..367481 (+) 267 WP_132301900.1 GNAT family N-acetyltransferase -
  O1Q83_RS01800 (O1Q83_00359) - 367889..369289 (+) 1401 WP_132301898.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 470 a.a.        Molecular weight: 52765.31 Da        Isoelectric Point: 6.2327

>NTDB_id=1058278 O1Q83_RS01770 WP_132301910.1 363049..364461(+) (pilB) [Lonepinella koalarum strain ACM3666]
MQASLSNPNVVSVDGTIFQISSQLWQQNQQQQAILIRYLACPLAEDESCLWLGLDSLNNLSACETFSFLTGKMIEPVLLE
TQVLKDLLQALAPQSLMVQESPVFYHVQQQDLNEEKHSAQQSDEPVIQLLNRIFESALQQRASDIHLEPQSQHLQVRFRI
DGVLQYQSPVALALSSRVISRLKLLAKLDISETRLPQDGRFHFKTTFSDILDFRLSTLPTPLGEKAVLRVQQNKPVQFAF
ADLGMNDQQQERFKHALNQPQGLILVTGPTGSGKSITLYTALSYLNRPEKHIMTAEDPIEIPLSGIIQSQVNPQIGLDFS
RLLRTFLRQDPDIIMLGEIRDEESAAMALRASQTGHLVLSTLHTNDAPSAIPRLLQLGIKQHEIDNSLLLVIAQRLIRKR
CQKCGGKSEHSCDCHQGYKGRIGIYQFLQPDPEQGYQLDFNQLQQAGLQKVEQGVTDLAEIQRVLGQGTK

Nucleotide


Download         Length: 1413 bp        

>NTDB_id=1058278 O1Q83_RS01770 WP_132301910.1 363049..364461(+) (pilB) [Lonepinella koalarum strain ACM3666]
ATGCAAGCCAGTTTATCTAACCCAAATGTTGTCAGTGTCGATGGAACAATATTTCAAATTTCATCGCAGTTATGGCAACA
AAATCAGCAACAACAGGCAATTTTAATCCGCTATCTGGCTTGTCCATTGGCAGAAGATGAATCTTGTTTGTGGCTTGGGT
TGGATAGCCTCAACAATTTATCCGCATGTGAAACCTTTTCTTTCCTAACAGGAAAAATGATCGAACCTGTGTTGTTGGAA
ACACAGGTATTGAAAGATTTATTGCAAGCCTTAGCTCCACAATCTTTAATGGTACAAGAAAGTCCGGTTTTTTACCATGT
TCAACAGCAAGATTTAAACGAAGAAAAGCATTCTGCTCAACAATCAGATGAACCCGTGATTCAGTTGCTGAATCGGATTT
TTGAAAGTGCGTTGCAGCAACGAGCATCTGATATTCACCTTGAGCCACAAAGCCAACATTTGCAAGTGCGTTTTCGTATT
GATGGTGTATTACAATACCAATCGCCAGTTGCCTTGGCATTATCTAGCAGGGTGATTTCACGTTTAAAATTATTAGCCAA
ACTTGATATTAGCGAAACCCGTTTACCGCAAGACGGACGTTTTCATTTTAAAACCACCTTTTCTGATATTTTAGATTTTC
GTCTTTCCACCTTACCAACCCCATTAGGGGAAAAAGCGGTATTGCGGGTGCAACAAAATAAGCCAGTACAATTTGCATTT
GCCGATCTGGGTATGAATGATCAGCAGCAGGAACGTTTTAAACACGCCCTTAATCAACCGCAAGGTTTGATTTTGGTGAC
AGGACCTACGGGAAGTGGAAAAAGTATCACCCTTTACACCGCACTTTCTTATTTAAATCGTCCAGAAAAACATATTATGA
CCGCGGAAGATCCTATTGAGATTCCATTGTCTGGCATTATTCAAAGCCAAGTCAATCCACAAATTGGTTTAGATTTTAGT
CGGTTATTGCGTACTTTTTTACGCCAAGATCCCGATATTATTATGCTCGGTGAAATTCGTGATGAAGAAAGTGCCGCCAT
GGCATTACGAGCTTCTCAAACGGGACATCTCGTGCTTTCCACGCTGCATACCAATGATGCGCCGTCCGCTATTCCACGCT
TGTTACAGTTAGGTATAAAGCAACACGAAATTGACAACAGCTTATTGTTAGTGATTGCACAACGTCTGATTCGGAAACGT
TGCCAAAAGTGCGGTGGAAAAAGCGAACATTCTTGTGATTGTCATCAGGGCTATAAAGGGCGAATCGGGATTTATCAATT
TTTACAACCTGATCCAGAGCAAGGTTATCAGTTGGATTTTAATCAGTTACAACAAGCAGGGTTGCAAAAGGTGGAGCAAG
GTGTGACGGATTTAGCGGAAATTCAACGAGTATTAGGGCAGGGGACAAAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(132-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4R1KY20

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.308

99.362

0.679
  pilB Haemophilus influenzae Rd KW20

67.597

99.149

0.67
  pilB Glaesserella parasuis strain SC1401

59.607

97.447

0.581
  pilB Vibrio campbellii strain DS40M4

40.956

100

0.419
  pilB Vibrio parahaemolyticus RIMD 2210633

40.541

100

0.415
  pilB Acinetobacter baylyi ADP1

38.632

100

0.409
  pilB Acinetobacter baumannii D1279779

38.98

100

0.406
  pilB Vibrio cholerae strain A1552

39.212

100

0.402
  pilB Legionella pneumophila strain ERS1305867

38.65

100

0.402
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.082

100

0.372

Multiple sequence alignment