Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACFJIW_RS23360 Genome accession   NZ_CP170554
Coordinates   5604456..5606183 (+) Length   575 a.a.
NCBI ID   WP_386067614.1    Uniprot ID   -
Organism   Tahibacter sp. UC22_41     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5599456..5611183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJIW_RS23340 (ACFJIW_23340) asnB 5599589..5601472 (-) 1884 WP_386067607.1 asparagine synthase (glutamine-hydrolyzing) -
  ACFJIW_RS23345 (ACFJIW_23345) - 5601501..5602685 (-) 1185 WP_386067609.1 glycosyltransferase -
  ACFJIW_RS23350 (ACFJIW_23350) - 5602678..5603433 (-) 756 WP_386067610.1 class I SAM-dependent methyltransferase -
  ACFJIW_RS23355 (ACFJIW_23355) - 5603430..5604278 (-) 849 WP_386067612.1 glycosyltransferase family 2 protein -
  ACFJIW_RS23360 (ACFJIW_23360) pilB 5604456..5606183 (+) 1728 WP_386067614.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFJIW_RS23365 (ACFJIW_23365) pilC 5606187..5607467 (+) 1281 WP_386067616.1 type II secretion system F family protein Machinery gene
  ACFJIW_RS23370 (ACFJIW_23370) pilD 5607478..5608353 (+) 876 WP_386067618.1 prepilin peptidase Machinery gene
  ACFJIW_RS23375 (ACFJIW_23375) coaE 5608350..5608979 (+) 630 WP_386067619.1 dephospho-CoA kinase -
  ACFJIW_RS23380 (ACFJIW_23380) - 5608981..5609961 (-) 981 WP_386067621.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62052.14 Da        Isoelectric Point: 6.4172

>NTDB_id=1057973 ACFJIW_RS23360 WP_386067614.1 5604456..5606183(+) (pilB) [Tahibacter sp. UC22_41]
MATQINPANLAGLSGIARRLVAEGSLAEPDARRAVETSAKQKIPLATYLIDNGMADSRVVAMASSAEFGVPLYDVNAVDF
SQLPLKLVSEELITKHRALPLFKRGNRLFVGLSDPTNVRALDEIKFQSNHSVEPILIDEQGLARAIEQALSQSSASIASE
GDEDLESLDLNAGDDDGDANSGGVDATGSDDTPIVRFVNKVLVDAIKRGASDIHFEPFETEYRVRLRMDGMLRKVAAPPT
KLAPRISSRLKVMSGLDIAERRVPQDGRIKLNLSKGKSIDFRVSTCPTLFGEKVVLRVLDGSAAKLGIEKLGYEDDQKKL
FLDAIEKPYGMVLVTGPTGSGKTVSLYTALNILNTEGRNISTVEDPVEIRVPGINQVQQNQKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTISRLMNMGIAPFNITSSVTLVIAQRLARRLHDCKRPHVLPPA
ALLAEGFTEQEIATLQMFDAVGCSGCNEGYKGRVGVYQVMPLAEDIAKIILAGGNAIQIAEAARSAGIRDLRASAILKVK
QGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1057973 ACFJIW_RS23360 WP_386067614.1 5604456..5606183(+) (pilB) [Tahibacter sp. UC22_41]
ATGGCTACGCAGATCAATCCGGCGAATCTCGCCGGCCTGAGCGGCATCGCACGACGCCTCGTCGCCGAAGGTTCGCTCGC
GGAACCCGATGCCCGGCGCGCCGTGGAAACCTCGGCCAAGCAGAAGATTCCCCTCGCCACTTATCTGATCGACAACGGCA
TGGCCGACAGCCGTGTCGTGGCCATGGCGTCTTCGGCGGAGTTCGGCGTACCGCTCTACGACGTCAACGCGGTCGACTTC
AGCCAGCTGCCGCTCAAGCTCGTCAGCGAAGAACTCATTACCAAGCATCGCGCGCTGCCGCTGTTCAAGCGCGGCAACCG
CCTGTTCGTCGGCCTGTCGGACCCGACCAACGTACGCGCGCTGGACGAGATCAAGTTCCAGTCCAACCACAGCGTCGAAC
CCATCCTCATCGACGAGCAGGGTCTGGCACGGGCCATCGAACAGGCCCTGAGCCAGAGCAGCGCGTCGATCGCCAGCGAA
GGCGACGAGGACCTGGAGAGCCTGGACCTCAACGCCGGCGACGACGACGGCGATGCGAACTCCGGCGGCGTCGACGCCAC
CGGCAGCGACGACACGCCGATCGTGCGCTTCGTCAACAAGGTGCTCGTCGACGCGATCAAGCGCGGCGCCTCGGACATCC
ACTTCGAACCGTTCGAAACCGAATACCGCGTCCGCCTGCGCATGGACGGCATGCTGCGCAAGGTCGCCGCGCCGCCGACC
AAGCTCGCGCCGCGCATCTCCTCGCGCCTCAAGGTCATGTCCGGCCTGGACATCGCCGAGCGCCGCGTGCCGCAGGACGG
CCGCATCAAGCTCAACCTGTCCAAGGGCAAGTCGATCGACTTCCGCGTCAGCACCTGCCCGACCCTGTTCGGCGAAAAGG
TCGTGCTGCGCGTGCTCGACGGCTCGGCGGCCAAGCTCGGCATCGAGAAGCTCGGCTACGAGGACGACCAGAAAAAGCTG
TTCCTCGATGCGATCGAGAAGCCCTACGGCATGGTGCTCGTGACCGGCCCGACCGGCTCGGGCAAGACCGTGTCGCTGTA
TACGGCGCTGAACATCCTCAACACCGAAGGCCGCAACATCTCCACGGTCGAGGATCCGGTCGAAATCCGCGTGCCCGGCA
TCAACCAGGTGCAGCAGAACCAGAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGACCCGGAC
GTCATCATGGTCGGCGAGATCCGCGACCTCGAAACCGCGGAGATCGCCGTCAAGGCGGCGCAGACCGGCCACATGGTGCT
GTCGACGCTGCACACGAACGACGCGCCGCAGACCATCTCGCGCCTGATGAACATGGGCATCGCGCCGTTCAACATCACCT
CGTCGGTGACCCTCGTCATCGCCCAGCGTCTCGCGCGGCGCCTGCACGACTGCAAGAGGCCTCACGTGCTGCCGCCGGCG
GCGCTGCTGGCCGAAGGCTTCACCGAGCAGGAAATCGCGACCCTGCAGATGTTCGACGCGGTCGGCTGCTCCGGCTGCAA
CGAAGGTTACAAGGGGCGCGTCGGCGTCTATCAGGTCATGCCCCTGGCCGAAGACATCGCCAAGATCATCCTGGCGGGCG
GCAATGCGATCCAGATCGCCGAGGCGGCACGCTCCGCCGGCATTCGCGACCTGCGCGCATCGGCGATCCTGAAGGTCAAA
CAGGGCGTCACGAGCCTCGCCGAAATCAACCGCGTCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.477

98.435

0.546
  pilB Acinetobacter baylyi ADP1

55.124

98.435

0.543
  pilB Legionella pneumophila strain ERS1305867

52.465

98.783

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

46.597

99.652

0.464
  pilB Vibrio cholerae strain A1552

52.353

88.696

0.464
  pilB Vibrio campbellii strain DS40M4

47.173

98.435

0.464
  pilF Neisseria gonorrhoeae MS11

48.435

94.435

0.457
  pilF Thermus thermophilus HB27

37.876

98.261

0.372
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.888

92.87

0.37

Multiple sequence alignment