Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACFJIW_RS23370 Genome accession   NZ_CP170554
Coordinates   5607478..5608353 (+) Length   291 a.a.
NCBI ID   WP_386067618.1    Uniprot ID   -
Organism   Tahibacter sp. UC22_41     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5602478..5613353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJIW_RS23350 (ACFJIW_23350) - 5602678..5603433 (-) 756 WP_386067610.1 class I SAM-dependent methyltransferase -
  ACFJIW_RS23355 (ACFJIW_23355) - 5603430..5604278 (-) 849 WP_386067612.1 glycosyltransferase family 2 protein -
  ACFJIW_RS23360 (ACFJIW_23360) pilB 5604456..5606183 (+) 1728 WP_386067614.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFJIW_RS23365 (ACFJIW_23365) pilC 5606187..5607467 (+) 1281 WP_386067616.1 type II secretion system F family protein Machinery gene
  ACFJIW_RS23370 (ACFJIW_23370) pilD 5607478..5608353 (+) 876 WP_386067618.1 prepilin peptidase Machinery gene
  ACFJIW_RS23375 (ACFJIW_23375) coaE 5608350..5608979 (+) 630 WP_386067619.1 dephospho-CoA kinase -
  ACFJIW_RS23380 (ACFJIW_23380) - 5608981..5609961 (-) 981 WP_386067621.1 Nudix family hydrolase -
  ACFJIW_RS23385 (ACFJIW_23385) secA 5609991..5612720 (-) 2730 WP_386067623.1 preprotein translocase subunit SecA -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 31749.72 Da        Isoelectric Point: 8.1379

>NTDB_id=1057975 ACFJIW_RS23370 WP_386067618.1 5607478..5608353(+) (pilD) [Tahibacter sp. UC22_41]
MIELFGSGWPLYLAAGLFGLLVGSFLNVVILRLPARLMHDWRTQSRELLGLDVDITAPAEQAPPDLVFKSSHCPHCKHTL
SPLDNIPLLSWLALRGRCRYCRAPISAQYPLIELLTAVATVVVVWQFGFSLQAAAGIALSYLLIALSGIDFRTQLLPDNL
TLPLLWLGLLLSLQPLFVDPVDAILGASIGYLSLWTVYWAFKLATGKEGMGHGDFKLLGALGAWMGPRSLLPIILLSSLV
GAILGSIILATRGKDRSTPIPFGPFIAVAGWVQFIWGEQLLRLYTSLLLTP

Nucleotide


Download         Length: 876 bp        

>NTDB_id=1057975 ACFJIW_RS23370 WP_386067618.1 5607478..5608353(+) (pilD) [Tahibacter sp. UC22_41]
ATGATCGAGCTCTTCGGCAGCGGCTGGCCGCTCTATCTGGCGGCCGGCCTCTTCGGCCTGCTGGTCGGCAGCTTCCTCAA
CGTCGTGATCCTGCGCCTGCCGGCGCGGCTCATGCACGACTGGCGCACGCAGAGCCGCGAGCTGCTCGGCCTGGACGTCG
ACATCACCGCGCCGGCCGAACAGGCGCCGCCGGACCTGGTGTTCAAGAGTTCGCACTGCCCGCACTGCAAGCACACGCTG
TCGCCGCTGGACAACATTCCGCTGCTGAGCTGGCTCGCGCTGCGCGGCCGCTGCCGCTACTGCCGCGCGCCGATCTCGGC
GCAGTACCCGCTGATCGAACTGCTGACCGCGGTGGCGACCGTCGTCGTGGTCTGGCAATTCGGCTTTTCGCTGCAGGCCG
CCGCCGGCATCGCGCTGAGCTATCTGCTGATCGCGCTGTCGGGCATCGACTTCCGCACCCAGCTGCTGCCCGACAACCTG
ACCTTGCCGCTGCTCTGGCTCGGTCTGCTGCTGTCGCTGCAGCCGCTGTTCGTCGACCCGGTCGACGCGATACTCGGCGC
AAGCATCGGCTATCTGAGCCTGTGGACCGTGTACTGGGCGTTCAAGCTCGCCACCGGCAAGGAAGGCATGGGCCACGGCG
ACTTCAAGCTGCTCGGCGCGCTCGGTGCCTGGATGGGGCCGCGCAGCCTGCTACCGATCATCCTGCTGTCGTCGCTGGTC
GGCGCGATTCTCGGCAGCATCATCCTGGCCACACGCGGCAAGGACCGCTCCACGCCGATTCCGTTCGGCCCGTTCATCGC
GGTCGCCGGCTGGGTGCAGTTCATCTGGGGCGAACAGCTGCTGCGTCTGTATACGAGCCTGCTGCTGACGCCATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.313

96.564

0.505
  pilD Vibrio campbellii strain DS40M4

50

97.595

0.488
  pilD Acinetobacter nosocomialis M2

50.365

94.158

0.474
  pilD Acinetobacter baumannii D1279779

50

94.158

0.471
  pilD Neisseria gonorrhoeae MS11

43.657

92.096

0.402

Multiple sequence alignment