Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFJY4_RS01515 Genome accession   NZ_CP170500
Coordinates   336336..337481 (+) Length   381 a.a.
NCBI ID   WP_020305662.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain MBI-RS3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 331336..342481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJY4_RS01495 (ACFJY4_01495) - 333051..333641 (-) 591 WP_014598006.1 YggT family protein -
  ACFJY4_RS01500 (ACFJY4_01500) proC 333651..334475 (-) 825 WP_045159852.1 pyrroline-5-carboxylate reductase -
  ACFJY4_RS01505 (ACFJY4_01505) - 334486..335175 (-) 690 WP_340067464.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFJY4_RS01510 (ACFJY4_01510) pilT 335242..336276 (+) 1035 WP_011914970.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFJY4_RS01515 (ACFJY4_01515) pilU 336336..337481 (+) 1146 WP_020305662.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFJY4_RS01520 (ACFJY4_01520) - 337478..338311 (-) 834 WP_125865994.1 TIGR03915 family putative DNA repair protein -
  ACFJY4_RS01525 (ACFJY4_01525) - 338311..339525 (-) 1215 WP_049340316.1 putative DNA modification/repair radical SAM protein -
  ACFJY4_RS01530 (ACFJY4_01530) - 339661..340056 (+) 396 WP_011914966.1 TM2 domain-containing protein -
  ACFJY4_RS01535 (ACFJY4_01535) - 340114..341388 (-) 1275 WP_386414399.1 dihydroorotase -
  ACFJY4_RS01540 (ACFJY4_01540) - 341388..342404 (-) 1017 WP_011914964.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42538.82 Da        Isoelectric Point: 6.8858

>NTDB_id=1057766 ACFJY4_RS01515 WP_020305662.1 336336..337481(+) (pilU) [Stutzerimonas stutzeri strain MBI-RS3]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYAQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDEDPGRRFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1057766 ACFJY4_RS01515 WP_020305662.1 336336..337481(+) (pilU) [Stutzerimonas stutzeri strain MBI-RS3]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACGGCTGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACGCCCATGTCGCCGGAGATGACCCGGGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCACGAATGCAACTTCGCCATCAGTGCCCGCGGCATCGGCCGC
TTCCGTGTCAGCGCGTTCTATCAGCGAAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
AGAGCTCAAGCTGCCGGACGTCCTGAAGAAGCTGTCGATGACCAAACGTGGTCTGGTGCTGTTCGTCGGCGCCACCGGTA
CCGGCAAGTCGACGTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGGCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCACCAGAATTGCATCGTCACCCAGCGCGAAGTGGGCATCGACACCAGCTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGTACCCGCGAAACCATGGATTACG
CGGTCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCGGACCGGCACAACCAGGTCTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACGCCTGACGGCAAGGGCCGTCGTGCGGTGATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAATCGCTGATGAAGCGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACGCGCAGGGGGAGATCACCTACGAAGATGCCCTGCTGCATGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGGGTTCGGAAACCGACGGTGAGCACCTGACCAGCGTATCCCAGGGCCTGAGCCTGGAGGTCAGCGACG
AGGACCCGGGCCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

94.751

100

0.948
  pilU Acinetobacter baylyi ADP1

66.106

93.701

0.619
  pilU Vibrio cholerae strain A1552

59.143

91.864

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

88.976

0.391
  pilT Pseudomonas aeruginosa PAK

41.194

87.927

0.362
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.828

88.714

0.362
  pilT Vibrio cholerae strain A1552

40.828

88.714

0.362

Multiple sequence alignment