Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BCT87_RS04170 Genome accession   NZ_CP170031
Coordinates   1013437..1015122 (+) Length   561 a.a.
NCBI ID   WP_065107043.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.261.52.C4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1008437..1020122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCT87_RS04150 (BCT87_04150) pdhR 1009925..1010686 (-) 762 WP_016768209.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  BCT87_RS04155 (BCT87_04155) ampD 1011168..1011767 (-) 600 WP_065107040.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCT87_RS04160 (BCT87_04160) nadC 1011851..1012741 (+) 891 WP_065107041.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCT87_RS04165 (BCT87_04165) - 1012996..1013436 (+) 441 WP_065107042.1 pilin -
  BCT87_RS04170 (BCT87_04170) pilB 1013437..1015122 (+) 1686 WP_065107043.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BCT87_RS04175 (BCT87_04175) pilC 1015138..1016367 (+) 1230 WP_016799551.1 type II secretion system F family protein Machinery gene
  BCT87_RS04180 (BCT87_04180) pilD 1016434..1017303 (+) 870 WP_016795167.1 prepilin peptidase Machinery gene
  BCT87_RS04185 (BCT87_04185) coaE 1017306..1017920 (+) 615 WP_010435920.1 dephospho-CoA kinase -
  BCT87_RS04190 (BCT87_04190) zapD 1017947..1018687 (+) 741 WP_010435917.1 cell division protein ZapD -
  BCT87_RS04195 (BCT87_04195) yacG 1018769..1018966 (+) 198 WP_010435916.1 DNA gyrase inhibitor YacG -
  BCT87_RS04200 (BCT87_04200) rplS 1019241..1019594 (-) 354 WP_004735514.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62037.73 Da        Isoelectric Point: 5.2005

>NTDB_id=1054821 BCT87_RS04170 WP_065107043.1 1013437..1015122(+) (pilB) [Vibrio cyclitrophicus strain 10N.261.52.C4]
MLTNLPTVLRQAGLLSLTQEQAVAEQVQASGISTPEALLVLDFFSGDSLANNIKTIFGLPLVQLANTDYESLCEQLGLRE
LITKYHAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVKVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEAQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSASETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKTADDSPDIYLRHSIPNSQT
IYKANSQGCNECNQGYSGRVGIYEVMPFTDKLKTSLINKPNALAIEDLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
I

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1054821 BCT87_RS04170 WP_065107043.1 1013437..1015122(+) (pilB) [Vibrio cyclitrophicus strain 10N.261.52.C4]
GTGCTCACTAACCTCCCAACCGTTCTACGTCAGGCTGGCTTACTTAGCCTGACTCAAGAACAAGCCGTTGCGGAACAAGT
ACAAGCTTCAGGCATTTCGACACCTGAAGCTTTGCTCGTTCTAGATTTCTTCAGCGGCGATTCCTTAGCAAACAACATTA
AAACCATCTTCGGCTTACCGTTAGTACAACTTGCCAATACGGACTATGAATCCTTGTGCGAACAGTTAGGGCTTCGCGAG
CTAATCACTAAATATCACGCTATTCCGATTTCAGTTTCTAGCTCCACTCTCACACTCGCTTCTGCCGACCCTACGGATTT
ACAAGCTGAAGATGACTTTCGATTCGCAACCGGATTACAGATTGAATTGGTTGTCGCTAATTACTCTGAATTAGAAGGCG
CAATACGCAAGCTGTATGGTCGATCTATTTCCGGGCAAGATTCTAAGCGTAAAGAGATCACCCAAGATGAGCTAGCTAAC
CTCGTGAAGGTATCTGACGACGAAATAACGTCGATTGAAGATCTCAGCCAAGACGACTCCCCTGTCAGTCGCTTTATCAA
TCAGATACTGGTTGATGCGGTGCGTAAAGGTGCTTCTGATATTCACTTCGAACCCTACGAAGAACACTACCGAGTGCGCC
TACGTTGCGATGGTATATTGGTTGAAATCCAACAGCCCGCCTCTCATTTAAGCCGTCGCCTGTCAGCTCGCTTAAAGATT
CTCGCTAAACTCGATATTGCCGAACGTCGCTTACCTCAAGATGGCCGTATTAAACTGCGTTTAAATGACGAACTGGCAAT
TGATATGCGTGTATCTACGCTGCCAACATTGTGGGGAGAAAAGATCGTATTGCGATTGCTTGATAGCAGTGCGGCCAATC
TCGATATCGATAAGCTCGGTTACAGCGAGGCTCAAAAAGCCCTCTACCTCAATGCACTAAAGCGCCCGCAAGGGATGATC
TTAATGACCGGGCCAACCGGCAGCGGTAAAACGGTTTCTCTTTATACTGGGCTTCGCGTGCTTAACACCTCTGAACGCAA
CATCTCAACAGCTGAAGACCCTGTAGAGATTAACCTGTGTGGCATCAATCAGGTACAAGTGACGCCTAAGATTGGCTTTG
GTTTCGCCGAGGCACTACGATCGTTTTTACGACAAGACCCAGACATCGTGATGGTTGGAGAAATCCGAGATTTAGAAACC
GCAGAAATCGCGATCAAAGCATCGCAAACAGGTCACTTAGTGCTTTCTACACTGCACACCAATTCTGCTTCTGAAACCGT
AACTCGGCTTGCTCACATGGGAATTGAACCCTTTAATCTTGCTTCGTCTTTAAGCTTGATTATTGCTCAGCGTCTTGCGA
GGCGCTTGTGTAACCACTGTAAAACCGCAGACGACTCACCAGACATTTATCTGCGTCACTCTATCCCAAACAGCCAAACC
ATCTACAAAGCCAACTCTCAAGGCTGTAATGAGTGTAACCAAGGTTACTCAGGTCGAGTGGGTATCTATGAAGTGATGCC
GTTTACCGACAAGCTAAAAACCAGCCTTATCAATAAACCCAATGCGCTCGCGATTGAAGATCTCGCACGTCGAGAAGGCA
TGCGCACTCTGCAAGAGTCAGGTCTGGATAAATTGCTTGAAGGCACCACCAGCTATCAAGAGCTGCAACGTGTTCTGTAC
ATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(10-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.936

100

0.743
  pilB Vibrio campbellii strain DS40M4

70.819

100

0.709
  pilB Vibrio parahaemolyticus RIMD 2210633

70.641

100

0.708
  pilB Legionella pneumophila strain ERS1305867

47.74

98.574

0.471
  pilB Acinetobacter baumannii D1279779

50.482

92.513

0.467
  pilB Acinetobacter baylyi ADP1

49.031

91.979

0.451
  pilF Neisseria gonorrhoeae MS11

43.262

100

0.435
  pilF Thermus thermophilus HB27

38.772

92.87

0.36

Multiple sequence alignment