Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BCV14_RS13090 Genome accession   NZ_CP170011
Coordinates   2976717..2978402 (+) Length   561 a.a.
NCBI ID   WP_102363584.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.54.E4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2971717..2983402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCV14_RS13070 (BCV14_13070) pdhR 2973174..2973935 (-) 762 WP_016768209.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  BCV14_RS13075 (BCV14_13075) ampD 2974417..2975016 (-) 600 WP_016790757.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCV14_RS13080 (BCV14_13080) nadC 2975100..2975990 (+) 891 WP_004738163.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCV14_RS13085 (BCV14_13085) pilA 2976244..2976717 (+) 474 WP_016790756.1 pilin Machinery gene
  BCV14_RS13090 (BCV14_13090) pilB 2976717..2978402 (+) 1686 WP_102363584.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BCV14_RS13095 (BCV14_13095) pilC 2978417..2979646 (+) 1230 WP_102363585.1 type II secretion system F family protein Machinery gene
  BCV14_RS13100 (BCV14_13100) pilD 2979713..2980582 (+) 870 WP_102363586.1 prepilin peptidase Machinery gene
  BCV14_RS13105 (BCV14_13105) coaE 2980585..2981199 (+) 615 WP_016794575.1 dephospho-CoA kinase -
  BCV14_RS13110 (BCV14_13110) zapD 2981226..2981966 (+) 741 WP_010435917.1 cell division protein ZapD -
  BCV14_RS13115 (BCV14_13115) yacG 2982048..2982245 (+) 198 WP_102363587.1 DNA gyrase inhibitor YacG -
  BCV14_RS13120 (BCV14_13120) rplS 2982517..2982870 (-) 354 WP_004735514.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62104.87 Da        Isoelectric Point: 5.4344

>NTDB_id=1054472 BCV14_RS13090 WP_102363584.1 2976717..2978402(+) (pilB) [Vibrio cyclitrophicus strain 10N.286.54.E4]
MLTNLPTVLRQAGLLSLTQEQAVAEHVEASGISTPEALLALNFFSSDSLANNIKTIFGLPLVRLANTDYESLCEQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQVELVVANYSELEGAIRKLYGRSISGQYSKRKEITQDELAN
LVKVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEAQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTSERNISTAEDPIEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKTADDSPDIYLRHSIPNSQT
IYKANSQGCNECNQGYSGRVGIYEVMPFTDKLKTSLINKPNALAIEDLARREGMRTLQESGIDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1054472 BCV14_RS13090 WP_102363584.1 2976717..2978402(+) (pilB) [Vibrio cyclitrophicus strain 10N.286.54.E4]
ATGTTAACCAACCTCCCAACCGTTCTCCGTCAGGCTGGCTTACTTAGCCTGACTCAGGAACAAGCTGTTGCGGAACATGT
GGAAGCTTCAGGTATATCTACACCTGAAGCTTTACTCGCTCTGAATTTCTTCAGCAGCGATTCCTTAGCAAACAACATTA
AAACCATCTTCGGCTTACCGTTAGTACGATTAGCCAATACTGACTACGAATCCCTGTGTGAACAATTAGGACTTCGTGAA
CTGATTACTAAGTATCGTGCTATTCCAATTTCAGTTTCTAGCTCAACTCTCACACTCGCTTCTGCCGACCCTACGGATTT
ACAGGCTGAAGATGACTTCCGCTTTGCGACAGGATTGCAAGTTGAATTGGTTGTCGCTAACTACTCTGAATTAGAGGGTG
CAATACGAAAATTGTATGGTCGTTCTATTTCCGGGCAATACTCCAAACGCAAAGAGATCACCCAAGATGAGCTAGCTAAC
CTCGTCAAGGTATCTGACGACGAAATAACGTCGATAGAAGATCTCAGCCAAGATGACTCTCCTGTGAGTCGCTTTATCAA
TCAGATACTGGTTGATGCGGTGCGTAAAGGCGCATCGGACATCCACTTCGAACCTTATGAAGAAAACTACCGCGTACGTC
TACGTTGCGATGGCATCCTTGTCGAGATCCAACAACCCGCCTCTCATTTAAGCCGTCGTCTGTCAGCTCGCTTAAAGATC
CTCGCTAAACTCGATATTGCCGAACGTCGCTTACCTCAAGATGGCCGTATCAAACTGCGTTTAAATGACGAACTGGCAAT
TGATATGCGCGTATCAACGCTGCCTACCTTATGGGGCGAGAAGATCGTACTGCGATTGCTTGATAGCAGTGCGGCCAATC
TCGATATAGACAAGCTCGGTTACAGCGAGGCTCAAAAAGCCCTCTACCTCAATGCACTAAAGCGCCCGCAAGGGATGATC
TTAATGACCGGGCCAACCGGCAGCGGTAAAACGGTTTCTCTTTATACTGGACTTCGAGTACTCAACACCTCTGAACGCAA
CATCTCAACAGCTGAAGACCCAATAGAGATTAACCTGTGTGGTATCAATCAGGTACAGGTGACGCCTAAGATTGGCTTTG
GTTTCGCCGAGGCACTACGATCGTTTTTACGACAAGACCCAGACATCGTGATGGTTGGAGAAATCCGAGATTTAGAAACC
GCCGAAATCGCGATCAAAGCCTCCCAAACCGGGCATTTAGTACTTTCAACACTGCACACTAACTCAGCCGCAGAAACCGT
GACCCGGCTTGCTCATATGGGAATCGAGCCCTTCAACCTTGCTTCGTCTTTGAGCTTGATTATTGCTCAGCGTCTTGCGA
GACGCTTGTGTAACCACTGTAAAACCGCAGACGACTCACCAGACATTTATCTGCGCCACTCTATCCCAAACAGCCAAACC
ATCTACAAAGCCAACTCTCAAGGCTGTAATGAATGTAACCAAGGTTACTCAGGTCGAGTCGGTATCTATGAAGTGATGCC
GTTTACCGACAAGCTAAAAACCAGCCTTATCAATAAACCCAATGCGCTAGCGATTGAAGATCTCGCACGTCGAGAAGGCA
TGAGAACTTTACAAGAGTCAGGGATAGATAAACTGCTTGAAGGCACCACCAGCTACCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.759

100

0.742
  pilB Vibrio campbellii strain DS40M4

71.174

100

0.713
  pilB Vibrio parahaemolyticus RIMD 2210633

70.996

100

0.711
  pilB Legionella pneumophila strain ERS1305867

48.007

98.396

0.472
  pilB Acinetobacter baumannii D1279779

50.482

92.513

0.467
  pilB Acinetobacter baylyi ADP1

49.336

93.939

0.463
  pilF Neisseria gonorrhoeae MS11

44.643

99.822

0.446

Multiple sequence alignment