Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BCV29_RS07470 Genome accession   NZ_CP170001
Coordinates   1731389..1733074 (+) Length   561 a.a.
NCBI ID   WP_016794572.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.55.B4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1726389..1738074
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCV29_RS07450 (BCV29_07450) pdhR 1727829..1728590 (-) 762 WP_016768209.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  BCV29_RS07455 (BCV29_07455) ampD 1729072..1729671 (-) 600 WP_016799547.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCV29_RS07460 (BCV29_07460) nadC 1729755..1730645 (+) 891 WP_102312962.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCV29_RS07465 (BCV29_07465) - 1730899..1731384 (+) 486 WP_016794571.1 pilin -
  BCV29_RS07470 (BCV29_07470) pilB 1731389..1733074 (+) 1686 WP_016794572.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BCV29_RS07475 (BCV29_07475) pilC 1733089..1734318 (+) 1230 WP_016794573.1 type II secretion system F family protein Machinery gene
  BCV29_RS07480 (BCV29_07480) pilD 1734385..1735254 (+) 870 WP_102312963.1 prepilin peptidase Machinery gene
  BCV29_RS07485 (BCV29_07485) coaE 1735257..1735871 (+) 615 WP_016794575.1 dephospho-CoA kinase -
  BCV29_RS07490 (BCV29_07490) zapD 1735898..1736638 (+) 741 WP_010435917.1 cell division protein ZapD -
  BCV29_RS07495 (BCV29_07495) yacG 1736720..1736917 (+) 198 WP_010435916.1 DNA gyrase inhibitor YacG -
  BCV29_RS07500 (BCV29_07500) rplS 1737196..1737549 (-) 354 WP_004735514.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62007.80 Da        Isoelectric Point: 5.3034

>NTDB_id=1054276 BCV29_RS07470 WP_016794572.1 1731389..1733074(+) (pilB) [Vibrio cyclitrophicus strain 10N.286.55.B4]
MLTNLPTVLRQAGLLSLTQEQAVAEHVEASGISTPEALLVLDLFSGDSLANNIKTIFGLPLVRLASTDYESLCEQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQVELVVANYSELEGAIRKLYGHSISGQNSKRKEITQDELAN
LVKVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYDEHFRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEAQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKETDDSPDIFLRHSIPNSQI
IYKANSQGCNECNQGYSGRVGIYEVMPFTDKLKTSLINKPNALAIEDLARREGMRTLQESGIDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1054276 BCV29_RS07470 WP_016794572.1 1731389..1733074(+) (pilB) [Vibrio cyclitrophicus strain 10N.286.55.B4]
ATGCTCACCAACCTCCCAACCGTTCTCCGTCAGGCTGGCTTACTTAGCCTGACTCAGGAACAAGCTGTTGCGGAACATGT
GGAAGCTTCAGGTATATCTACACCTGAAGCTTTGCTCGTTCTGGATTTGTTCAGCGGCGATTCCTTAGCAAACAACATTA
AAACCATCTTCGGCTTACCGTTAGTACGATTAGCCAGTACTGACTACGAATCCCTGTGTGAACAATTAGGGCTTCGTGAA
CTGATTACTAAGTATCGTGCTATTCCAATTTCAGTTTCTAGCTCAACTCTCACACTCGCTTCTGCCGACCCTACGGATTT
ACAGGCTGAAGATGACTTCCGCTTTGCGACAGGATTGCAAGTTGAATTAGTTGTCGCTAACTACTCTGAATTAGAGGGTG
CAATACGAAAATTGTATGGTCATTCTATTTCCGGGCAAAACTCTAAGCGTAAAGAGATCACCCAAGATGAACTAGCTAAC
CTCGTGAAGGTATCTGACGACGAAATAACGTCGATTGAAGATCTCAGCCAAGATGACTCTCCTGTGAGTCGCTTTATCAA
TCAGATCTTGGTTGATGCGGTACGTAAAGGCGCATCGGACATCCACTTCGAACCTTATGACGAACACTTCCGTGTGCGCC
TGCGTTGTGATGGCATATTGGTTGAAATCCAACAGCCCGCCTCTCATTTAAGCCGTCGCCTGTCAGCTCGCTTAAAGATT
CTCGCCAAACTCGATATCGCCGAACGTCGCTTACCTCAAGATGGCCGTATCAAACTGCGTTTAAATGACGAACTGGCAAT
TGATATGCGCGTATCAACGCTGCCTACCTTATGGGGCGAGAAGATCGTACTGCGATTGCTTGATAGCAGTGCGGCCAATC
TCGATATCGACAAGCTCGGTTACAGCGAGGCTCAAAAAGCCCTCTACCTCAATGCACTAAAGCGCCCGCAAGGGATGATC
TTAATGACCGGGCCAACCGGCAGCGGTAAAACGGTTTCTCTTTATACTGGACTTCGAGTACTCAACACCTCTGAACGCAA
CATCTCAACAGCTGAAGACCCTGTAGAGATTAACCTGTGTGGCATCAATCAGGTACAAGTGACGCCTAAGATTGGCTTTG
GTTTCGCCGAGGCACTACGATCGTTTTTACGACAAGACCCAGACATCGTGATGGTTGGAGAAATCCGAGATTTAGAAACC
GCCGAAATCGCGATCAAAGCCTCCCAAACCGGGCATTTAGTACTTTCAACACTGCACACTAACTCAGCCGCAGAAACCGT
GACCCGGCTTGCTCATATGGGAATCGAGCCCTTCAACCTTGCTTCGTCATTGAGCCTAATCATAGCCCAACGACTGGCAA
GACGCTTGTGCAACCATTGCAAAGAAACAGACGACTCGCCGGACATATTTCTGCGTCACTCGATTCCTAACAGCCAAATA
ATCTACAAAGCTAACTCTCAAGGGTGTAATGAATGTAACCAAGGTTACTCAGGTCGAGTGGGTATCTATGAAGTGATGCC
GTTTACCGACAAGCTAAAAACCAGCCTTATCAATAAACCCAATGCGCTAGCGATTGAAGATCTCGCACGTCGAGAAGGCA
TGAGAACTTTACAAGAGTCAGGGATAGATAAACTGCTTGAAGGCACCACCAGCTACCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.404

100

0.738
  pilB Vibrio campbellii strain DS40M4

70.463

100

0.706
  pilB Vibrio parahaemolyticus RIMD 2210633

70.285

100

0.704
  pilB Legionella pneumophila strain ERS1305867

47.826

98.396

0.471
  pilB Acinetobacter baumannii D1279779

50.674

92.513

0.469
  pilB Acinetobacter baylyi ADP1

49.808

93.048

0.463
  pilF Neisseria gonorrhoeae MS11

44.286

99.822

0.442
  pilF Thermus thermophilus HB27

39.155

92.87

0.364

Multiple sequence alignment