Detailed information NTG1053985

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACEZ45_RS12410
• Genome accession: NZ_CP169877
• Coordinates: 2550803..2551417 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain JF
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2545803..2556417

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACEZ45_RS12380 (ACEZ45_12380)	rpsT	2546103..2546369 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACEZ45_RS12385 (ACEZ45_12385)	holA	2546386..2547427 (-)	1042	Protein_2392	DNA polymerase III subunit delta	-
ACEZ45_RS12390 (ACEZ45_12390)	-	2547467..2547621 (-)	155	Protein_2393	hypothetical protein	-
ACEZ45_RS12395 (ACEZ45_12395)	-	2547662..2547796 (+)	135	WP_003152870.1	YqzM family protein	-
ACEZ45_RS12400 (ACEZ45_12400)	comEC	2547815..2550166 (-)	2352	WP_069473513.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACEZ45_RS12405 (ACEZ45_12405)	-	2550167..2550736 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACEZ45_RS12410 (ACEZ45_12410)	comEA	2550803..2551417 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACEZ45_RS12415 (ACEZ45_12415)	comER	2551476..2552297 (+)	822	WP_014305449.1	late competence protein ComER	-
ACEZ45_RS12420 (ACEZ45_12420)	-	2552366..2553103 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
ACEZ45_RS12425 (ACEZ45_12425)	rsfS	2553100..2553456 (-)	357	WP_003152864.1	ribosome silencing factor	-
ACEZ45_RS12430 (ACEZ45_12430)	yqeK	2553453..2554034 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACEZ45_RS12435 (ACEZ45_12435)	-	2554024..2554593 (-)	570	WP_058906195.1	nicotinate-nucleotide adenylyltransferase	-
ACEZ45_RS12440 (ACEZ45_12440)	yhbY	2554604..2554894 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACEZ45_RS12445 (ACEZ45_12445)	aroE	2554888..2555730 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=1053985 ACEZ45_RS12410 WP_003152867.1 2550803..2551417(-) (comEA) [Bacillus amyloliquefaciens strain JF]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1053985 ACEZ45_RS12410 WP_003152867.1 2550803..2551417(-) (comEA) [Bacillus amyloliquefaciens strain JF]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363