Detailed information NTG1053983

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: ACEZ45_RS12400
• Genome accession: NZ_CP169877
• Coordinates: 2547815..2550166 (-)
• Length: 783 a.a.
• NCBI ID: WP_069473513.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain JF
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2542815..2555166

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACEZ45_RS12365 (ACEZ45_12365)	-	2543178..2543516 (-)	339	WP_003152882.1	YqxA family protein	-
ACEZ45_RS12370 (ACEZ45_12370)	spoIIP	2543533..2544726 (-)	1194	WP_003152880.1	stage II sporulation protein P	-
ACEZ45_RS12375 (ACEZ45_12375)	gpr	2544794..2545900 (-)	1107	WP_003152878.1	GPR endopeptidase	-
ACEZ45_RS12380 (ACEZ45_12380)	rpsT	2546103..2546369 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACEZ45_RS12385 (ACEZ45_12385)	holA	2546386..2547427 (-)	1042	Protein_2392	DNA polymerase III subunit delta	-
ACEZ45_RS12390 (ACEZ45_12390)	-	2547467..2547621 (-)	155	Protein_2393	hypothetical protein	-
ACEZ45_RS12395 (ACEZ45_12395)	-	2547662..2547796 (+)	135	WP_003152870.1	YqzM family protein	-
ACEZ45_RS12400 (ACEZ45_12400)	comEC	2547815..2550166 (-)	2352	WP_069473513.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACEZ45_RS12405 (ACEZ45_12405)	-	2550167..2550736 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACEZ45_RS12410 (ACEZ45_12410)	comEA	2550803..2551417 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACEZ45_RS12415 (ACEZ45_12415)	comER	2551476..2552297 (+)	822	WP_014305449.1	late competence protein ComER	-
ACEZ45_RS12420 (ACEZ45_12420)	-	2552366..2553103 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
ACEZ45_RS12425 (ACEZ45_12425)	rsfS	2553100..2553456 (-)	357	WP_003152864.1	ribosome silencing factor	-
ACEZ45_RS12430 (ACEZ45_12430)	yqeK	2553453..2554034 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACEZ45_RS12435 (ACEZ45_12435)	-	2554024..2554593 (-)	570	WP_058906195.1	nicotinate-nucleotide adenylyltransferase	-
ACEZ45_RS12440 (ACEZ45_12440)	yhbY	2554604..2554894 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-

Sequence

Protein

• Length: 783 a.a.
• Molecular weight: 86666.68 Da
• Isoelectric Point: 9.0078

>NTDB_id=1053983 ACEZ45_RS12400 WP_069473513.1 2547815..2550166(-) (comEC) [Bacillus amyloliquefaciens strain JF]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIMIKTKQPAPVVVCLVSFCVYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATIPGGFDYKEYLYSQQI
HWLFTVTSIQQCEKSKQPLFKLLSIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YLLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQRISLVSFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFDMFDLVMKPVHDFITYAASVDLFTMIVLKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGKSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPKLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFEKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide

• Length: 2352 bp

>NTDB_id=1053983 ACEZ45_RS12400 WP_069473513.1 2547815..2550166(-) (comEC) [Bacillus amyloliquefaciens strain JF]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATGATAAAAACAAAGCAGCCTGCTCCGGTTGTTGTCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGGTCTCTTGAGGAAAAGAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGATTCCCGGGGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTACCGTGACTTCTATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAGCATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAAGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGACTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGGATATTGCTGCTGCT
GTTTTTGCCGGTCTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCTCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACCTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCCTCATTCATCGCAGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCGAATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTTTCAAGGCAGATGGGAGAATGTTTGTTTGATATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTTAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTAGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGCCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCGCTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAGGATCACATCGGGGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAAGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACAGAAGTGCTGAAAACGTATCCGAAACTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCACGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTGAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (508-719)

• Competence protein (Competence) (208-469)

• Domain of unknown function (DUF4131) (DUF4131) (25-171)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	56.477	98.595	0.557