Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACEWX4_RS13210 Genome accession   NZ_CP169369
Coordinates   2747459..2748517 (-) Length   352 a.a.
NCBI ID   WP_374855093.1    Uniprot ID   -
Organism   Acinetobacter indicus strain AIBD14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2742459..2753517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEWX4_RS13185 (ACEWX4_13185) aroK 2742923..2743465 (-) 543 WP_075167858.1 shikimate kinase AroK -
  ACEWX4_RS13190 (ACEWX4_13190) pilQ 2743502..2745565 (-) 2064 WP_374855090.1 type IV pilus secretin PilQ Machinery gene
  ACEWX4_RS13195 (ACEWX4_13195) pilP 2745581..2746108 (-) 528 WP_045795346.1 pilus assembly protein PilP Machinery gene
  ACEWX4_RS13200 (ACEWX4_13200) pilO 2746108..2746827 (-) 720 WP_005180513.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACEWX4_RS13205 (ACEWX4_13205) pilN 2746824..2747459 (-) 636 WP_005180516.1 PilN domain-containing protein Machinery gene
  ACEWX4_RS13210 (ACEWX4_13210) pilM 2747459..2748517 (-) 1059 WP_374855093.1 pilus assembly protein PilM Machinery gene
  ACEWX4_RS13215 (ACEWX4_13215) ponA 2748689..2751265 (+) 2577 WP_180006566.1 penicillin-binding protein PBP1a -
  ACEWX4_RS13220 (ACEWX4_13220) - 2751306..2752610 (-) 1305 WP_152342363.1 IS4 family transposase -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38980.63 Da        Isoelectric Point: 4.6106

>NTDB_id=1051079 ACEWX4_RS13210 WP_374855093.1 2747459..2748517(-) (pilM) [Acinetobacter indicus strain AIBD14]
MRRLYRKPSKGLIGVDISSTSVKLLELSVKNDRYWVESYALVPLSEGSVVEKNILNPEAVGDALERAFNLANTPTTAAAL
AIPTSMVISKTIEMDADMTDDEREVQIRMDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRLENVESRCEAIEL
AGLTPKVADVESFAIENAFKVFADTLPMGVNTIGVLDIGHTMTTLSVLKDNKIIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKSRTLPDDYDVEVLEPFLEAVVQQAARSLQFFFSSSQFHEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSSKIDIKRIESDASSLMVACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=1051079 ACEWX4_RS13210 WP_374855093.1 2747459..2748517(-) (pilM) [Acinetobacter indicus strain AIBD14]
GTGCGCAGGTTATATCGTAAACCGAGTAAGGGGTTAATAGGTGTAGATATTAGTTCTACTTCTGTTAAGTTATTGGAGCT
CTCTGTAAAGAATGATCGTTACTGGGTGGAAAGTTACGCCTTGGTACCGCTCTCAGAGGGCAGTGTGGTTGAGAAAAATA
TTCTGAATCCTGAAGCTGTAGGGGATGCGCTCGAGCGTGCCTTTAATTTGGCCAATACACCGACAACAGCTGCCGCTTTA
GCGATTCCAACCTCAATGGTAATTAGCAAGACCATTGAGATGGATGCCGATATGACGGATGACGAACGTGAAGTTCAAAT
CCGGATGGATGCAGAGCAATATATTCCTTTCCCATTAGATGAAGTTAGCCTGGACTTTGAGGTGCTGCCTGATCGTCTGG
CCAATCCAAACCGGGTCAATGTTTTGCTGGTGGCGACACGACTGGAAAATGTCGAGAGTCGCTGTGAAGCGATTGAACTG
GCCGGTTTAACCCCAAAAGTGGCAGATGTTGAAAGCTTTGCCATTGAAAATGCCTTTAAAGTCTTCGCCGATACCTTGCC
GATGGGGGTAAATACCATTGGTGTGTTAGACATTGGTCATACTATGACCACGCTGTCGGTACTCAAAGACAATAAAATTA
TCTATACCCGCGAGCAGGTCTTTGGTGGCAAGCAGCTGACCCAGGAAATCCAGAACCGTTATGGTCTGTCCTTTGAAGAA
GCCGGCCGCGCCAAGAAAAGCCGTACCTTGCCAGATGATTATGATGTTGAAGTGTTGGAGCCATTCCTTGAGGCGGTGGT
GCAGCAGGCGGCACGTTCGCTGCAGTTCTTCTTCTCCTCATCGCAGTTTCATGAAATTGACCATATTCTGCTGGCCGGTG
GGAATGCCAATATTCCAGGCCTAGCCAAGCTACTACAGCAAAAACTGGGTTACCGCGTCACCATTGCCAATCCGTTTTTA
CAGATGGGTTTCTCCTCCAAAATTGATATTAAAAGAATTGAAAGCGATGCCTCTTCATTGATGGTGGCATGTGGCTTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

83.807

100

0.838
  comM Acinetobacter nosocomialis M2

83.807

100

0.838
  comM Acinetobacter baylyi ADP1

78.409

100

0.784
  pilM Legionella pneumophila strain ERS1305867

40.96

100

0.412

Multiple sequence alignment