Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACEWX4_RS13200 Genome accession   NZ_CP169369
Coordinates   2746108..2746827 (-) Length   239 a.a.
NCBI ID   WP_005180513.1    Uniprot ID   N9KYM2
Organism   Acinetobacter indicus strain AIBD14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2741108..2751827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEWX4_RS13180 (ACEWX4_13180) aroB 2741818..2742900 (-) 1083 WP_374855088.1 3-dehydroquinate synthase -
  ACEWX4_RS13185 (ACEWX4_13185) aroK 2742923..2743465 (-) 543 WP_075167858.1 shikimate kinase AroK -
  ACEWX4_RS13190 (ACEWX4_13190) pilQ 2743502..2745565 (-) 2064 WP_374855090.1 type IV pilus secretin PilQ Machinery gene
  ACEWX4_RS13195 (ACEWX4_13195) pilP 2745581..2746108 (-) 528 WP_045795346.1 pilus assembly protein PilP Machinery gene
  ACEWX4_RS13200 (ACEWX4_13200) pilO 2746108..2746827 (-) 720 WP_005180513.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACEWX4_RS13205 (ACEWX4_13205) pilN 2746824..2747459 (-) 636 WP_005180516.1 PilN domain-containing protein Machinery gene
  ACEWX4_RS13210 (ACEWX4_13210) pilM 2747459..2748517 (-) 1059 WP_374855093.1 pilus assembly protein PilM Machinery gene
  ACEWX4_RS13215 (ACEWX4_13215) ponA 2748689..2751265 (+) 2577 WP_180006566.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 239 a.a.        Molecular weight: 27087.71 Da        Isoelectric Point: 5.1311

>NTDB_id=1051077 ACEWX4_RS13200 WP_005180513.1 2746108..2746827(-) (pilO) [Acinetobacter indicus strain AIBD14]
MSLDQYEEMNQDPVAAPKKKMTLDKFLQQWNTLDPNNYGSWPFSVKLTIWIFIIILIAFLGYFLAIKPKLDAISNARAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLQQLPKETEIPGLVEDVNVTGVNSGLRFKNIRLENEIKQEFFIEQP
ISIEATGDYHAFGSFVSGTAALPRIVTMHDFTITAGENKEKKTDIPQITYVAKAKTYRYVGNTDAQAGNSTTPATGAAQ

Nucleotide


Download         Length: 720 bp        

>NTDB_id=1051077 ACEWX4_RS13200 WP_005180513.1 2746108..2746827(-) (pilO) [Acinetobacter indicus strain AIBD14]
ATGAGTCTCGATCAATATGAAGAAATGAATCAGGATCCTGTTGCTGCGCCAAAGAAAAAAATGACGCTGGATAAATTCCT
GCAGCAATGGAATACCTTAGATCCGAATAACTATGGCAGCTGGCCGTTTTCCGTCAAGCTGACCATCTGGATCTTTATCA
TTATTTTAATTGCCTTCCTGGGCTATTTCCTGGCCATCAAGCCGAAGCTGGATGCAATTTCCAATGCGCGTGCACAAGAG
CAGAACCTGCTGAATGAGTTTCGTGAAAAAGATTCCAAACTGCGTAACTTGCAGCAATATCAGGCACAGTTACAGGAAAT
GGAAGCCAACTTTAACCAGCAGCTACAACAGTTGCCAAAAGAAACCGAAATTCCAGGTCTGGTTGAAGATGTCAACGTTA
CGGGCGTGAATTCAGGCTTAAGATTTAAAAATATCCGTCTGGAAAATGAAATCAAGCAAGAGTTCTTTATCGAGCAGCCA
ATTTCGATTGAAGCCACCGGTGATTATCATGCTTTTGGTTCTTTTGTGAGCGGTACAGCCGCTTTACCGCGAATTGTGAC
CATGCATGATTTCACTATTACTGCCGGTGAAAACAAGGAAAAGAAAACCGATATTCCACAAATTACCTATGTCGCGAAAG
CCAAGACCTATCGCTATGTCGGGAATACCGATGCACAGGCAGGGAATAGCACGACTCCAGCCACAGGAGCAGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9KYM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

69.748

99.582

0.695
  comO Acinetobacter baylyi ADP1

67.213

100

0.686

Multiple sequence alignment