Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O1Q82_RS08910 Genome accession   NZ_CP169367
Coordinates   1892444..1893892 (-) Length   482 a.a.
NCBI ID   WP_386687256.1    Uniprot ID   -
Organism   Lonepinella sp. MS14437     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1887444..1898892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1Q82_RS08880 (O1Q82_01772) - 1887631..1888998 (-) 1368 WP_386687246.1 alanine/glycine:cation symporter family protein -
  O1Q82_RS08885 (O1Q82_01773) - 1889411..1889677 (-) 267 WP_386687248.1 GNAT family N-acetyltransferase -
  O1Q82_RS08890 (O1Q82_01774) yacG 1889680..1889880 (-) 201 WP_132301902.1 DNA gyrase inhibitor YacG -
  O1Q82_RS08895 (O1Q82_01775) coaE 1889864..1890493 (-) 630 WP_386687251.1 dephospho-CoA kinase -
  O1Q82_RS08900 (O1Q82_01776) - 1890519..1891181 (-) 663 WP_386687252.1 prepilin peptidase -
  O1Q82_RS08905 (O1Q82_01777) pilC 1891214..1892434 (-) 1221 WP_386687254.1 type II secretion system F family protein Machinery gene
  O1Q82_RS08910 (O1Q82_01778) pilB 1892444..1893892 (-) 1449 WP_386687256.1 GspE/PulE family protein Machinery gene
  O1Q82_RS08915 (O1Q82_01779) pilA 1893892..1894377 (-) 486 WP_386687258.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  O1Q82_RS08920 (O1Q82_01780) ampD 1894522..1895067 (+) 546 WP_386687260.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  O1Q82_RS08925 (O1Q82_01781) - 1895071..1895721 (+) 651 WP_386687262.1 HAD family hydrolase -
  O1Q82_RS08930 (O1Q82_01782) - 1895973..1896242 (+) 270 WP_153984892.1 PTS sugar transporter subunit IIB -
  O1Q82_RS08935 (O1Q82_01783) - 1896252..1897607 (+) 1356 WP_153984893.1 PTS ascorbate transporter subunit IIC -
  O1Q82_RS08940 (O1Q82_01784) - 1897608..1898426 (+) 819 WP_386687265.1 transketolase -

Sequence

Protein


Download         Length: 482 a.a.        Molecular weight: 54250.12 Da        Isoelectric Point: 6.8128

>NTDB_id=1051005 O1Q82_RS08910 WP_386687256.1 1892444..1893892(-) (pilB) [Lonepinella sp. MS14437]
MQESLSSPHIVSSNVVSPNVVSPNVVSHDGKIFQISPQLWQQNQQQQNILIRYLACPLAENERCLWLGLDSLNNLSACET
FSFLTGKMIEPVLLETQVLKDLLQALAPQSLMVQESPVFYHVQQQDLNEEKHSTQQSDEPVIQLLNRIFESALQQRASDI
HLEPQSQHLQVRFRIDGVLQHQPPVALALSSRVISRLKLLAKLDISETRLPQDGRFHFKTTFSDILDFRLSTLPTPFGEK
AVLRVQQNKPVQFAFADLGMNDQQQERFKHALNQPQGLILVTGPTGSGKSITLYTALSYLNRPEKHIMTAEDPIEIPLSG
IIQSQVNPQIGLDFSRLLRTFLRQDPDIIMLGEIRDEESAAMALRASQTGHLVLSTLHTNDAPSAIPRLLQLGIKQHEID
NSLLLVIAQRLIRKRCQKCGGKSENSCDCHQGYKGRIGIYQFLQPDPKQGYQLDVDHLQQAGLEKVKQGITDLVEIQRVL
GH

Nucleotide


Download         Length: 1449 bp        

>NTDB_id=1051005 O1Q82_RS08910 WP_386687256.1 1892444..1893892(-) (pilB) [Lonepinella sp. MS14437]
ATGCAAGAGAGTCTATCTAGTCCACATATTGTTAGCTCAAATGTTGTTAGCCCGAATGTTGTTAGTCCAAATGTGGTGAG
CCATGACGGAAAAATATTCCAAATTTCACCGCAGTTATGGCAACAAAATCAGCAACAACAGAACATTCTGATCCGTTATT
TAGCTTGTCCATTAGCGGAAAATGAGCGTTGTTTATGGCTCGGGCTAGATAGCCTCAACAATTTATCCGCTTGTGAAACC
TTTTCTTTCCTAACAGGAAAAATGATCGAACCTGTGTTGTTGGAAACACAGGTATTGAAAGATTTATTGCAAGCCTTAGC
TCCACAATCTTTAATGGTGCAAGAAAGCCCTGTTTTTTACCATGTTCAACAGCAAGATTTAAACGAAGAAAAGCATTCTA
CTCAACAATCAGATGAACCCGTGATTCAGTTGCTTAATCGGATTTTTGAAAGTGCGTTGCAGCAACGAGCATCTGATATT
CACCTTGAGCCACAAAGCCAACATTTGCAAGTGCGTTTTCGTATTGATGGTGTATTACAACACCAACCACCAGTTGCGTT
GGCATTATCTAGCAGGGTGATTTCACGTTTAAAATTATTAGCCAAACTCGATATTAGCGAAACTCGCTTACCGCAAGACG
GACGTTTTCATTTTAAAACCACCTTTTCTGATATTTTAGATTTTCGTCTTTCCACCTTACCAACCCCATTCGGGGAAAAG
GCAGTGTTGCGGGTGCAACAAAATAAGCCAGTACAATTTGCATTTGCCGATCTGGGTATGAATGATCAGCAGCAGGAACG
TTTTAAACACGCCCTTAATCAACCGCAAGGCTTGATTTTAGTGACAGGACCTACGGGAAGTGGGAAAAGTATCACACTTT
ACACCGCACTTTCTTATTTAAATCGTCCTGAAAAACATATTATGACCGCGGAAGATCCTATTGAAATTCCATTGTCTGGC
ATTATTCAAAGCCAAGTCAATCCACAAATTGGGTTAGATTTTAGTCGGTTATTGCGAACCTTTCTCCGTCAAGATCCCGA
TATTATTATGCTCGGTGAAATTCGTGATGAAGAAAGTGCCGCAATGGCATTACGAGCTTCTCAAACGGGACATCTCGTGC
TTTCCACGTTGCATACCAATGATGCACCGTCCGCTATTCCACGCTTGTTACAGTTAGGTATAAAGCAACACGAAATTGAC
AACAGCTTATTGTTAGTGATTGCACAACGTCTGATTCGGAAACGTTGCCAAAAGTGCGGTGGAAAAAGCGAAAATTCTTG
TGATTGTCATCAAGGCTATAAAGGTCGAATCGGGATTTATCAATTTTTACAACCTGATCCAAAGCAAGGTTATCAGTTGG
ATGTTGATCATTTACAACAAGCAGGGCTAGAGAAAGTGAAGCAAGGAATAACGGATTTAGTGGAAATTCAACGAGTATTA
GGACATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(147-414)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

70.241

94.813

0.666
  pilB Haemophilus influenzae Rd KW20

69.365

94.813

0.658
  pilB Glaesserella parasuis strain SC1401

59.041

95.228

0.562
  pilB Vibrio parahaemolyticus RIMD 2210633

39.961

100

0.429
  pilB Vibrio campbellii strain DS40M4

41.529

100

0.417
  pilB Acinetobacter baylyi ADP1

36.862

100

0.405
  pilB Legionella pneumophila strain ERS1305867

39.388

100

0.4
  pilB Vibrio cholerae strain A1552

39.05

100

0.392
  pilB Acinetobacter baumannii D1279779

38.193

100

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.667

99.585

0.365

Multiple sequence alignment