Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   O1Q82_RS08915 Genome accession   NZ_CP169367
Coordinates   1893892..1894377 (-) Length   161 a.a.
NCBI ID   WP_386687258.1    Uniprot ID   -
Organism   Lonepinella sp. MS14437     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1888892..1899377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1Q82_RS08885 (O1Q82_01773) - 1889411..1889677 (-) 267 WP_386687248.1 GNAT family N-acetyltransferase -
  O1Q82_RS08890 (O1Q82_01774) yacG 1889680..1889880 (-) 201 WP_132301902.1 DNA gyrase inhibitor YacG -
  O1Q82_RS08895 (O1Q82_01775) coaE 1889864..1890493 (-) 630 WP_386687251.1 dephospho-CoA kinase -
  O1Q82_RS08900 (O1Q82_01776) - 1890519..1891181 (-) 663 WP_386687252.1 prepilin peptidase -
  O1Q82_RS08905 (O1Q82_01777) pilC 1891214..1892434 (-) 1221 WP_386687254.1 type II secretion system F family protein Machinery gene
  O1Q82_RS08910 (O1Q82_01778) pilB 1892444..1893892 (-) 1449 WP_386687256.1 GspE/PulE family protein Machinery gene
  O1Q82_RS08915 (O1Q82_01779) pilA 1893892..1894377 (-) 486 WP_386687258.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  O1Q82_RS08920 (O1Q82_01780) ampD 1894522..1895067 (+) 546 WP_386687260.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  O1Q82_RS08925 (O1Q82_01781) - 1895071..1895721 (+) 651 WP_386687262.1 HAD family hydrolase -
  O1Q82_RS08930 (O1Q82_01782) - 1895973..1896242 (+) 270 WP_153984892.1 PTS sugar transporter subunit IIB -
  O1Q82_RS08935 (O1Q82_01783) - 1896252..1897607 (+) 1356 WP_153984893.1 PTS ascorbate transporter subunit IIC -
  O1Q82_RS08940 (O1Q82_01784) - 1897608..1898426 (+) 819 WP_386687265.1 transketolase -
  O1Q82_RS08945 (O1Q82_01785) - 1898423..1899367 (+) 945 WP_386687267.1 transketolase family protein -

Sequence

Protein


Download         Length: 161 a.a.        Molecular weight: 16944.26 Da        Isoelectric Point: 8.4488

>NTDB_id=1051006 O1Q82_RS08915 WP_386687258.1 1893892..1894377(-) (pilA) [Lonepinella sp. MS14437]
MYFNRTLIQTSYNLQFKKGFTLIELMIVIAIVAILATIAIPSYQNYTKKAAISELLQAAAPYRSEVELCVYNLDKPDNCS
AGQNGIQANITDGSGKYVKSVAVSAGKITVTGKGSVADVSYTLTASGSASTGVTWAANCGTKSELFPAGFCSTNSTSTGQ
Q

Nucleotide


Download         Length: 486 bp        

>NTDB_id=1051006 O1Q82_RS08915 WP_386687258.1 1893892..1894377(-) (pilA) [Lonepinella sp. MS14437]
ATGTATTTCAACCGCACTTTGATTCAAACTTCGTACAATCTACAGTTCAAAAAAGGCTTTACCCTGATTGAATTGATGAT
CGTGATTGCCATTGTGGCAATTTTAGCCACCATTGCAATTCCATCTTATCAAAATTATACCAAAAAAGCCGCTATTTCTG
AACTGTTGCAAGCAGCAGCACCTTATCGTTCCGAAGTAGAGCTTTGTGTTTATAACTTAGATAAACCAGATAATTGCTCC
GCAGGTCAAAATGGGATCCAAGCAAATATCACAGATGGTTCAGGCAAATATGTGAAATCTGTTGCCGTCAGTGCTGGCAA
GATTACCGTGACGGGTAAAGGTAGTGTTGCTGATGTGAGTTACACTTTAACTGCCAGCGGTTCAGCTAGTACAGGGGTAA
CTTGGGCGGCAAACTGCGGTACAAAATCGGAATTATTTCCAGCTGGTTTCTGTAGCACAAATAGTACAAGCACTGGACAA
CAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(49-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(17-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

66.423

85.093

0.565
  pilA Haemophilus influenzae Rd KW20

62.774

85.093

0.534
  pilA Glaesserella parasuis strain SC1401

54.545

88.82

0.484

Multiple sequence alignment