Detailed information NTG1050471

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACEY2B_RS03045
• Genome accession: NZ_CP169315
• Coordinates: 580633..581280 (+)
• Length: 215 a.a.
• NCBI ID: WP_015426939.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain IFLLLBEK1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 575633..586280

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACEY2B_RS03020 (ACEY2B_03020)	-	575762..576940 (+)	1179	WP_015426943.1	SLC13 family permease	-
ACEY2B_RS03025 (ACEY2B_03025)	-	577113..577676 (+)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
ACEY2B_RS03030 (ACEY2B_03030)	-	577780..578178 (+)	399	WP_015426942.1	hypothetical protein	-
ACEY2B_RS03035 (ACEY2B_03035)	-	578283..579215 (+)	933	WP_015426941.1	ABC transporter ATP-binding protein	-
ACEY2B_RS03040 (ACEY2B_03040)	-	579212..580573 (+)	1362	WP_374788218.1	ABC transporter permease	-
ACEY2B_RS03045 (ACEY2B_03045)	comEA	580633..581280 (+)	648	WP_015426939.1	ComEA family DNA-binding protein	Machinery gene
ACEY2B_RS03050 (ACEY2B_03050)	comEC	581261..583471 (+)	2211	WP_374788219.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACEY2B_RS03055 (ACEY2B_03055)	-	583756..584532 (+)	777	WP_015426937.1	alpha/beta hydrolase family protein	-
ACEY2B_RS03060 (ACEY2B_03060)	-	584717..584932 (+)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
ACEY2B_RS03065 (ACEY2B_03065)	atpB	584977..585690 (+)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
ACEY2B_RS03070 (ACEY2B_03070)	atpF	585705..586211 (+)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23622.83 Da
• Isoelectric Point: 5.0062

>NTDB_id=1050471 ACEY2B_RS03045 WP_015426939.1 580633..581280(+) (comEA) [Lactococcus lactis strain IFLLLBEK1]
MDKILEKVKEYWKMIVLIVCGLIAGGIFYVLTNGQKPTTNLSVEKLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVAKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=1050471 ACEY2B_RS03045 WP_015426939.1 580633..581280(+) (comEA) [Lactococcus lactis strain IFLLLBEK1]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAATTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAAATTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTGGCAAAACCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.123	100	0.381