Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACEVB2_RS07885 Genome accession   NZ_CP169223
Coordinates   1644685..1645119 (+) Length   144 a.a.
NCBI ID   WP_150332210.1    Uniprot ID   -
Organism   Pasteurella multocida subsp. gallicida strain W24_252     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1639685..1650119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEVB2_RS07855 (ACEVB2_07855) tadA 1639795..1640283 (-) 489 WP_108511809.1 tRNA adenosine(34) deaminase TadA -
  ACEVB2_RS07860 (ACEVB2_07860) - 1640310..1641161 (-) 852 WP_126417629.1 thymidylate synthase -
  ACEVB2_RS07865 (ACEVB2_07865) lgt 1641167..1641970 (-) 804 WP_115097854.1 prolipoprotein diacylglyceryl transferase -
  ACEVB2_RS07870 (ACEVB2_07870) - 1641980..1642774 (-) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  ACEVB2_RS07875 (ACEVB2_07875) rppH 1642777..1643373 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  ACEVB2_RS07880 (ACEVB2_07880) ampD 1643959..1644525 (-) 567 WP_203331640.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACEVB2_RS07885 (ACEVB2_07885) pilA 1644685..1645119 (+) 435 WP_150332210.1 prepilin peptidase-dependent pilin Machinery gene
  ACEVB2_RS07890 (ACEVB2_07890) pilB 1645119..1646507 (+) 1389 WP_150332209.1 GspE/PulE family protein Machinery gene
  ACEVB2_RS07895 (ACEVB2_07895) - 1646507..1647718 (+) 1212 WP_324007555.1 type II secretion system F family protein -
  ACEVB2_RS07900 (ACEVB2_07900) - 1647718..1648440 (+) 723 WP_374091455.1 prepilin peptidase -
  ACEVB2_RS07905 (ACEVB2_07905) coaE 1648455..1649075 (+) 621 WP_374091456.1 dephospho-CoA kinase -
  ACEVB2_RS07910 (ACEVB2_07910) yacG 1649086..1649289 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  ACEVB2_RS07915 (ACEVB2_07915) - 1649289..1649558 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15028.45 Da        Isoelectric Point: 9.1281

>NTDB_id=1049910 ACEVB2_RS07885 WP_150332210.1 1644685..1645119(+) (pilA) [Pasteurella multocida subsp. gallicida strain W24_252]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGGLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1049910 ACEVB2_RS07885 WP_150332210.1 1644685..1645119(+) (pilA) [Pasteurella multocida subsp. gallicida strain W24_252]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCGGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGGG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTGAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.735

100

0.375
  pilA Vibrio cholerae strain A1552

36.735

100

0.375
  pilA Vibrio cholerae C6706

36.735

100

0.375

Multiple sequence alignment