Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEVB2_RS07890 Genome accession   NZ_CP169223
Coordinates   1645119..1646507 (+) Length   462 a.a.
NCBI ID   WP_150332209.1    Uniprot ID   -
Organism   Pasteurella multocida subsp. gallicida strain W24_252     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1640119..1651507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEVB2_RS07860 (ACEVB2_07860) - 1640310..1641161 (-) 852 WP_126417629.1 thymidylate synthase -
  ACEVB2_RS07865 (ACEVB2_07865) lgt 1641167..1641970 (-) 804 WP_115097854.1 prolipoprotein diacylglyceryl transferase -
  ACEVB2_RS07870 (ACEVB2_07870) - 1641980..1642774 (-) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  ACEVB2_RS07875 (ACEVB2_07875) rppH 1642777..1643373 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  ACEVB2_RS07880 (ACEVB2_07880) ampD 1643959..1644525 (-) 567 WP_203331640.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACEVB2_RS07885 (ACEVB2_07885) pilA 1644685..1645119 (+) 435 WP_150332210.1 prepilin peptidase-dependent pilin Machinery gene
  ACEVB2_RS07890 (ACEVB2_07890) pilB 1645119..1646507 (+) 1389 WP_150332209.1 GspE/PulE family protein Machinery gene
  ACEVB2_RS07895 (ACEVB2_07895) - 1646507..1647718 (+) 1212 WP_324007555.1 type II secretion system F family protein -
  ACEVB2_RS07900 (ACEVB2_07900) - 1647718..1648440 (+) 723 WP_374091455.1 prepilin peptidase -
  ACEVB2_RS07905 (ACEVB2_07905) coaE 1648455..1649075 (+) 621 WP_374091456.1 dephospho-CoA kinase -
  ACEVB2_RS07910 (ACEVB2_07910) yacG 1649086..1649289 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  ACEVB2_RS07915 (ACEVB2_07915) - 1649289..1649558 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  ACEVB2_RS07920 (ACEVB2_07920) - 1650007..1651380 (+) 1374 WP_005722571.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 52213.84 Da        Isoelectric Point: 6.3686

>NTDB_id=1049911 ACEVB2_RS07890 WP_150332209.1 1645119..1646507(+) (pilB) [Pasteurella multocida subsp. gallicida strain W24_252]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHFSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCCDCS
VETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=1049911 ACEVB2_RS07890 WP_150332209.1 1645119..1646507(+) (pilB) [Pasteurella multocida subsp. gallicida strain W24_252]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGCGCAATGACAGGAGAGGTATTTGAAATCCAGCAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGCG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTGAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACACATTTAGGCGAAAAAGCGGTATTGCGTTTACAGCGAAATAAGCCTATTCATTTCAGTTTTGCTGAGTTGGGGATG
ACTGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCTCAAGGACTGATTTTAGTCACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTCAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAGGAAAGTGCGTTAATCGCATTACGTGCAGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTTGTGATTGCAGT
GTAGAGACACAATCGGCGTGTCGTTGCTATCAAGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAA
AACAGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACA
ATGAAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.991

98.052

0.667
  pilB Haemophilus influenzae Rd KW20

67.329

98.052

0.66
  pilB Glaesserella parasuis strain SC1401

58.811

98.268

0.578
  pilB Acinetobacter baumannii D1279779

39.351

100

0.42
  pilB Vibrio parahaemolyticus RIMD 2210633

39.501

100

0.411
  pilB Vibrio campbellii strain DS40M4

39.004

100

0.407
  pilB Acinetobacter baylyi ADP1

37.675

100

0.407
  pilB Vibrio cholerae strain A1552

38.066

100

0.4
  pilB Legionella pneumophila strain ERS1305867

37.014

100

0.392

Multiple sequence alignment