Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACETWS_RS05770 Genome accession   NZ_CP169073
Coordinates   1262569..1264311 (-) Length   580 a.a.
NCBI ID   WP_017405527.1    Uniprot ID   -
Organism   Delftia lacustris strain ZWP15     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1257569..1269311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACETWS_RS05740 (ACETWS_05740) mutT 1258151..1258657 (+) 507 WP_043789746.1 8-oxo-dGTP diphosphatase MutT -
  ACETWS_RS05745 (ACETWS_05745) - 1258668..1258922 (-) 255 WP_016445606.1 DNA gyrase inhibitor YacG -
  ACETWS_RS05750 (ACETWS_05750) zapD 1258922..1259677 (-) 756 WP_016445607.1 cell division protein ZapD -
  ACETWS_RS05755 (ACETWS_05755) coaE 1259767..1260405 (-) 639 WP_016445608.1 dephospho-CoA kinase -
  ACETWS_RS05760 (ACETWS_05760) pilD 1260420..1261313 (-) 894 WP_374006835.1 A24 family peptidase Machinery gene
  ACETWS_RS05765 (ACETWS_05765) pilC 1261313..1262530 (-) 1218 WP_016445610.1 type II secretion system F family protein Machinery gene
  ACETWS_RS05770 (ACETWS_05770) pilB 1262569..1264311 (-) 1743 WP_017405527.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACETWS_RS05780 (ACETWS_05780) - 1264548..1265477 (-) 930 WP_374008364.1 polyprenyl synthetase family protein -
  ACETWS_RS05785 (ACETWS_05785) rplU 1265708..1266019 (+) 312 WP_012207284.1 50S ribosomal protein L21 -
  ACETWS_RS05790 (ACETWS_05790) rpmA 1266035..1266292 (+) 258 WP_012207283.1 50S ribosomal protein L27 -
  ACETWS_RS05795 (ACETWS_05795) cgtA 1266442..1267557 (+) 1116 WP_016451026.1 Obg family GTPase CgtA -
  ACETWS_RS05800 (ACETWS_05800) proB 1267641..1268789 (+) 1149 WP_016445651.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62791.84 Da        Isoelectric Point: 5.1481

>NTDB_id=1049778 ACETWS_RS05770 WP_017405527.1 1262569..1264311(-) (pilB) [Delftia lacustris strain ZWP15]
MAVADSSLKNASAVASLPGIGRALISAGKVSQQAAEAAAGKAATNRTSFIAELSVAGTLSALEVAETVSTMFSAPLLDLD
AVDAMRLPQDLLDAKIISTYRVLPLSKRGNRLTVATADPTQQEAAEQIKFTTQLGVDWIIVEADKLERLITQQNKSASEA
MDSYGGSGDFEFGDLKPDEVAEEKDNSAAADVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIAS
PPIAIKEKLASRIKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARMGIDALGYEPEE
KARLLEAISRPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKEPAD
IPREALIDAGYDDADIDGSWVTYRPVGCPACNNGYKGRVGIYQVMPISEEIQRIILQDGSALEIAAQAKREGVRSLRGSG
LHKARQGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=1049778 ACETWS_RS05770 WP_017405527.1 1262569..1264311(-) (pilB) [Delftia lacustris strain ZWP15]
ATGGCTGTTGCTGACTCCTCGCTCAAGAATGCATCCGCTGTCGCATCGCTTCCCGGCATCGGGCGGGCCTTGATTTCAGC
AGGCAAGGTATCGCAACAGGCAGCGGAAGCGGCTGCCGGCAAGGCCGCCACCAATCGCACCTCGTTCATTGCAGAACTGA
GCGTCGCCGGCACCCTCTCTGCCCTGGAAGTGGCGGAGACGGTTTCCACCATGTTCAGCGCGCCGCTGCTCGATCTTGAT
GCGGTCGACGCCATGCGCCTGCCGCAGGATCTGCTGGACGCCAAGATCATCAGCACCTATCGCGTCCTGCCTCTGAGCAA
GCGCGGCAACCGCCTCACGGTGGCCACCGCCGACCCGACGCAGCAGGAAGCCGCCGAGCAGATCAAGTTCACCACCCAGT
TGGGCGTGGACTGGATCATCGTCGAGGCCGACAAGCTGGAGCGGCTGATCACGCAGCAGAACAAAAGCGCCAGCGAGGCC
ATGGACAGCTATGGCGGCAGCGGCGACTTCGAGTTCGGCGACCTCAAGCCTGATGAGGTGGCAGAGGAAAAGGACAACTC
GGCCGCAGCAGACGTCGAAGATGCGCCCATCGTCAAGTTCCTGCACAAAATGCTGCTCGACGCCTTCAACATGCGCGCGT
CGGACTTGCATTTCGAACCCTACGAGCACCAGTACCGCGTGCGCTTTCGCATTGACGGCGAGCTGCGTGAAATTGCATCG
CCGCCCATTGCCATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCGCGCATGGACATCTCGGAAAAGCGTGTCCC
GCAGGACGGGCGCATGAAGCTCAAGGTCGGCCCGGACCGCGTCATCGACTTTCGCGTCAGCACGCTGCCCACGCTTTTCG
GCGAGAAGATCGTGATCCGTATCCTGGACCCGAGCTCGGCCAGGATGGGCATCGACGCCCTGGGCTATGAACCCGAGGAG
AAGGCACGCCTGCTTGAAGCCATCAGCCGCCCCTACGGAATGATTCTGGTCACAGGCCCCACCGGCTCGGGCAAGACAGT
GTCGCTGTACACCTGCCTGAACCTGCTGAACAAGGCCGGCGTGAACATCGCCACGGCGGAAGATCCTTCGGAAATCAACC
TGCCCGGCGTCAACCAGGTCAATGTGAACGAAAAGGCCGGCCTCACCTTTGCCACTGCGCTCAAGGCTTTTCTGCGCCAG
GATCCCGACATCATCATGGTCGGTGAAATCCGCGATCTGGAAACGGCCGACATCTCGATCAAGGCAGCGCAAACCGGCCA
CTTGGTGCTGTCCACCCTGCACACCAATGACGCACCGACCACGTTGACGCGCATGCGCAACATGGGCATTGCGCCCTTCA
ACATCGCATCGAGCGTGATCCTGATCACCGCCCAGCGCCTGGCCAGGCGCCTGTGCCCTCAATGCAAGGAGCCGGCTGAC
ATTCCGCGGGAGGCCCTGATCGATGCAGGCTACGACGATGCGGACATTGACGGCAGCTGGGTCACCTACAGGCCCGTCGG
CTGTCCGGCATGCAACAACGGCTACAAGGGCCGCGTAGGCATCTATCAGGTGATGCCTATCAGCGAGGAAATCCAGCGCA
TCATCCTGCAGGACGGCAGTGCACTGGAGATCGCCGCACAGGCCAAGCGCGAAGGCGTGCGGTCGCTGCGTGGATCGGGT
TTGCACAAGGCCAGACAGGGACTGACCTCGCTCGAAGAAGTGCTGGCAGTCACCAATGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(57-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.206

97.241

0.547
  pilB Acinetobacter baylyi ADP1

55.712

98.103

0.547
  pilF Neisseria gonorrhoeae MS11

52.12

97.586

0.509
  pilB Legionella pneumophila strain ERS1305867

49.561

98.103

0.486
  pilB Vibrio cholerae strain A1552

47.038

96.034

0.452
  pilB Vibrio campbellii strain DS40M4

44.071

97.414

0.429
  pilB Vibrio parahaemolyticus RIMD 2210633

47.115

89.655

0.422
  pilF Thermus thermophilus HB27

39.062

99.31

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

83.793

0.362

Multiple sequence alignment