Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEQ85_RS06225 Genome accession   NZ_CP168966
Coordinates   1317976..1319688 (+) Length   570 a.a.
NCBI ID   WP_373883534.1    Uniprot ID   -
Organism   Acinetobacter pittii strain LS-AP     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1312976..1324688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEQ85_RS06190 (ACEQ85_06190) nusA 1313864..1315348 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  ACEQ85_RS06195 (ACEQ85_06195) rimP 1315386..1315910 (-) 525 WP_002114922.1 ribosome maturation factor RimP -
  ACEQ85_RS06215 (ACEQ85_06215) secG 1316548..1316877 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  ACEQ85_RS06220 (ACEQ85_06220) tpiA 1316890..1317684 (-) 795 WP_373883533.1 triose-phosphate isomerase -
  ACEQ85_RS06225 (ACEQ85_06225) pilB 1317976..1319688 (+) 1713 WP_373883534.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEQ85_RS06230 (ACEQ85_06230) pilC 1319715..1320941 (+) 1227 WP_032005095.1 type II secretion system F family protein Machinery gene
  ACEQ85_RS06235 (ACEQ85_06235) pilD 1320941..1321801 (+) 861 WP_373883535.1 A24 family peptidase Machinery gene
  ACEQ85_RS06240 (ACEQ85_06240) coaE 1321803..1322399 (+) 597 WP_311078292.1 dephospho-CoA kinase -
  ACEQ85_RS06245 (ACEQ85_06245) - 1322396..1323310 (-) 915 WP_005079347.1 DMT family transporter -
  ACEQ85_RS06250 (ACEQ85_06250) rlmB 1323346..1324095 (-) 750 WP_016142380.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACEQ85_RS06255 (ACEQ85_06255) - 1324198..1324524 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63593.00 Da        Isoelectric Point: 5.0338

>NTDB_id=1048975 ACEQ85_RS06225 WP_373883534.1 1317976..1319688(+) (pilB) [Acinetobacter pittii strain LS-AP]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVNLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDHKLVLKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILAQQFTEENSFEFSDEEF
DLDVDLEAPPSEEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKIPADIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1048975 ACEQ85_RS06225 WP_373883534.1 1317976..1319688(+) (pilB) [Acinetobacter pittii strain LS-AP]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGGTTAATCTCTCCC
CTTCTTTATTAGCTGAGACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACAAGCCAAATT
CCCAAAGATTTGGTCGATCATAAACTGGTTTTAAAACATCGGATCTTACCTCTTATACAAAGAGGACAGATTTTATATGT
CGCGACCAGCAACCCGAGCAATATTGAAGCGATTGATGCCATTCGCTTTAATAGCAAGCTCATGGTTGAACCAGTCATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGGCGCAGCAATTTACCGAAGAAAACAGCTTCGAATTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCACCCTCAGAAGAGGATGAGGAAGAAACGCCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCGATTCGTATGGGGGCTTCAGACTTACATTTTGAACCATACGAGAAATCAT
ATCGGGTTCGTTATCGAGTCGACGGCGTGTTGCGTCAGATTGCAAATCCGCCTTTGCAATTGGCTAACCGTTTAGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGAGTGCCTCAAGATGGTCGTATCAAGCTTAAGTTATCAAA
GACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCAATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACAGGTCCTACCGGTTCTGGTAAAACGGTATCTTTATATACTGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGCATTAATCAGGTTAACGTTAACCCAA
AAGTAGGTCTTACTTTTTCGGCCGCACTGAAATCATTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACCGGTCACATGGTAATGTCAACACTACATACCAATAGTGC
TCCAGAGACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACGTCTGTCAATTTGGTGATTGCTC
AACGTTTGGCGCGTCGGTTATGCCCACAGTGTAAAATACCTGCCGACATTCCTAAACAAAGCCTATTAGAAATGGGCTTT
ACCGAACAAGATTTAACCCATCCCGATTTTCAAGTTTTTCAACCTGTGGGTTGTACTGAATGTCGTGAAGGCTACAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGGGTTACTTCT
TTACAAGAAGTTAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.737

100

0.947
  pilB Acinetobacter baylyi ADP1

78.246

100

0.782
  pilB Legionella pneumophila strain ERS1305867

51.499

99.474

0.512
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.908

91.93

0.477
  pilB Vibrio campbellii strain DS40M4

50.674

91.053

0.461
  pilB Vibrio cholerae strain A1552

50.288

91.404

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.505

100

0.382
  pilF Thermus thermophilus HB27

36.833

98.596

0.363

Multiple sequence alignment